Anti-inflammatory Effect of Gentiopicroside in Adjuvant-induced Arthritis Rats

Xiao-qian XIE; He LI; Ya-le WANG; Jing-jing LIU; Zhi-jie WAN; Sha-sha LUO; Ze-yue ZHAO; Xiao-tian LI

doi:10.13422/j.cnki.syfjx.20200403

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Anti-inflammatory Effect of Gentiopicroside in Adjuvant-induced Arthritis Rats

Pharmacology | 更新时间：2021-02-09

- Anti-inflammatory Effect of Gentiopicroside in Adjuvant-induced Arthritis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 58-63(2020)
- 作者机构：
  
  1.郑州大学　药学院，郑州　 450001
  2.郑州大学　第一附属医院，郑州　 450052
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200403
  CLC： R2-0; R22; R285.5
- Received：25 July 2019，
  
  Published Online：06 November 2019，
  
  Published：20 March 2020
- 稿件说明：
移动端阅览
Xiao-qian XIE, He LI, Ya-le WANG, et al. Anti-inflammatory Effect of Gentiopicroside in Adjuvant-induced Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 58-63.
DOI：

Xiao-qian XIE, He LI, Ya-le WANG, et al. Anti-inflammatory Effect of Gentiopicroside in Adjuvant-induced Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 58-63. DOI： 10.13422/j.cnki.syfjx.20200403.

摘要

目的：

通过研究龙胆苦苷(GPS)对佐剂性关节炎大鼠(AA)的治疗作用，探讨其对于大鼠炎症反应的影响。

方法：

36只雄性SD大鼠随机分为6组，每组6只，分别为正常组，模型组，GPS低、中、高剂量组、甲氨蝶呤(MTX)组。除正常组，其余大鼠建立AA模型，在大鼠右后趾足垫内皮内注射0.1 mL完全弗氏佐剂(CFA)。造模成功后，各组连续给药7 d，1次/d，GPS低、中、高剂量组灌胃给药剂量为30，60，120 mg·kg

-1

，MTX组腹腔注射给药剂量为0.2 mg·kg

-1

，正常组与模型组灌胃给予等量的生理盐水。开始给药后每天使用数字游标卡尺及足趾容积测量仪记录各组大鼠的足厚度、足体积，第7天使用小动物活体成像以及苏木素-伊红染色的方法对大鼠的踝关节进行X射线检查和病理组织学检查。实验结束后大鼠腹主动脉取血，采用酶联免疫吸附测定(ELISA)试剂盒测定血清中白细胞介素-6(IL-6)，肿瘤坏死因子-

(TNF-

)的水平，采用实时荧光定量聚合酶链式反应(Real-time PCR)测定滑膜组织中IL-6，TNF-

mRNA水平。

结果：

与正常组比较，模型组大鼠大鼠足体积及足厚度，血清IL-6，TNF-

水平和滑膜组织中IL-6，TNF-

mRNA水平显著升高(

0.01)。与模型组比较，各给药组大鼠足体积及足厚度结果显著下降(

0.01)；血清TNF-

水平显著下降(

0.01)；滑膜组织中IL-6，TNF-

mRNA水平明显下降(

0.05，

0.01)。此外，X射线检查及病理组织学检查结果发现GPS对于AA大鼠的踝关节也有保护作用。

结论：

龙胆苦苷通过抑制血清IL-6，TNF-

水平及滑膜组织中IL-6，TNF-

mRNA水平发挥对AA大鼠的保护作用。

Abstract

Objective:

To study the therapeutic and inflammatory effects of gentiopicroside(GPS) on adjuvant-induced arthritis (AA) rats.

Method:

The 36 male SD rats were randomly divided into six groups

namely the model group

GPS groups (low

medium and high dose)

and the methotrexate (MTX) group

with six rats in each group. AA rats were induced through intradermal injection with 0.1 mL complete Freund's adjuvant (CFA) into the right hind paw

except the normal group. After modeling

rats in each group were treated with drugs for 7 days

once a day. The doses were 30

120 mg·kg

-1

in the GPS groups

and 0.2 mg·kg

-1

in the MTX group. The normal group and the model group were intragastrically treated with the same volume of normal saline. During the experiment

the paw thickness and paw volume of rats were recorded everyday by the digital vernier calipers and the toe volume measuring instrument. On the seventh day

X-ray and histopathological examination of the ankle joints were performed by the small animal living imaging instrument and hematoxylin eosin stain. Blood samples were collected from the abdominal aorta at the end of the experiment to determine the levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-

in serum by enzyme-linked immunosorbent assay(ELISA) kits. The mRNA levels of IL-6 and TNF-

in synovial tissues were determined by Real-time fluorescent quantitative polymerase chain reaction(Real-time PCR).

Result:

Compared with the normal group

the results of each index in the model group were significantly different (

0.01). Compared with the model group

the results of paw volume and paw thickness decreased significantly (

0.01)

TNF-

level decreased significantly (

0.01)

and IL-6 and TNF-

mRNA levels decreased significantly (

0.05

0.01) in drug treated groups. The results of X-ray and histopathological examinations indicated that GPS had a protective effect on the ankle joints of AA rats.

Conclusion:

GPS has the therapeutic effect on AA rats by inhibiting levels of proinflammatory cytokines in serum and relevant mRNA levels in synovial tissues.

关键词

Keywords

references

E M FIKRY , A M GAD , A H EID , et al . Caffeic acid and ellagic acid ameliorate adjuvant-induced arthritis in rats via targeting inflammatory signals, chitinase-3-like protein-1 and angiogenesis [J]. Biomed Pharmacother , 2019 , 110 : 878 - 886 .

J LIN , Y HE , B WANG , et al . Blocking of YY1 reduce neutrophil infiltration by inhibiting IL-8 production via the PI3K-Akt-mTOR signaling pathway in rheumatoid arthritis [J]. Clin Exp Immunol , 2019 , 195 ( 2 ): 226 - 236 .

K LIU , Y ZHANG , L LIU , et al . miR-125 regulates PI3K/Akt/mTOR signaling pathway in rheumatoid arthritis rats via PARP2 [J]. Biosci Rep , 2019 , doi: 10.1042/BSR20180890 http://dx.doi.org/10.1042/BSR20180890 .

M M N WENDT , M C DE OLIVEIRA , G B FRANCO-SALLA , et al . Fatty acids uptake and oxidation are increased in the liver of rats with adjuvant-induced arthritis [J]. Biochim Biophys Acta Mol Basis Dis , 2019 , 1865 ( 3 ): 696 - 707 .

C YU , X LI , Y HOU , et al . Hyaluronic acid coated acid-sensitive nanoparticles for targeted therapy of adjuvant-induced arthritis in rats [J]. Molecules , 2019 , 24 ( 1 ): E146 .

马占宾，张连生，王永东 . 浅析类风湿性关节炎的药物治疗 [J]. 医学综述， 2014 , 20 ( 12 ): 2221 - 2223 .

冯芳军，王香玲 . 类风湿性关节炎的研究进展 [J]. 中医杂志， 2011 ( S1 ): 201 - 203 .

H HAN , L XU , K XIONG , et al . Exploration of hepatoprotective effect of gentiopicroside on alpha-naphthylisothiocyanate-induced cholestatic liver injury in rats by comprehensive proteomic and metabolomic signatures [J]. Cell Physiol Biochem , 2018 , 49 ( 4 ): 1304 - 1319 .

C CHEN , Y Y WANG , Y X WANG , et al . Gentiopicroside ameliorates bleomycin-induced pulmonary fibrosis in mice via inhibiting inflammatory and fibrotic process [J]. Biochem Biophys Res Commun , 2018 , 495 ( 4 ): 2396 - 2403 .

X TANG , Q YANG , F YANG , et al . Target profiling analyses of bile acids in the evaluation of hepatoprotective effect of gentiopicroside on ANIT-induced cholestatic liver injury in mice [J]. J Ethnopharmacol , 2016 , 194 : 63 - 71 .

J LYU , W L GU , C X CHEN . Effect of gentiopicroside on experimental acute pancreatitis induced by retrograde injection of sodium taurocholate into the biliopancreatic duct in rats [J]. Fitoterapia , 2015 , 102 : 127 - 133 .

刘占文，陈长勋，金若敏，等 . 龙胆苦苷的保肝作用研究 [J]. 中草药， 2002 , 33 ( 1 ): 47 - 50 .

陈长勋，刘占文，孙峥嵘，等 . 龙胆苦苷抗炎药理作用研究 [J]. 中草药， 2003 , 34 ( 9 ): 814 - 816 .

郭海凤，朴惠顺 . 龙胆苦苷的提取工艺及药理作用研究进展 [J]. 延边大学医学学报， 2010 , 33 ( 1 ): 70 - 73 .

赵文娜，张爱军，梁杨静，等．龙胆苦苷对结肠炎小鼠的治疗作用 [J]. 西北药学杂志， 2017 ， 32 ( 5 ): 632 - 636 .

F CHEN , L XIE , R KANG , et al . Gentiopicroside inhibits RANKL-induced osteoclastogenesis by regulating NF- κ B and JNK signaling pathways [J]. Biomed Pharmacother , 2018 , 100 : 142 - 146 .

魏伟，吴希美，李元建 . 药理实验方法学 [M]．北京：人民卫生出版社， 2010 ： 749 - 750 .

M POHANKA , B RUTTKAY-NEDECKY , J FUSEK , et al . Melatonin regulates oxidative stress initiated by freund's complete adjuvant [J]. Acta Medica (Hradec Králové) , 2015 , 58 ( 1 ): 21 - 24 .

陈雷，王海波，孙晓丽，等 . 龙胆苦苷镇痛抗炎药理作用研究 [J]. 天然产物研究与开发， 2008 , 20 ( 5 ): 903 - 906 .

V G M NAIDU , B K R DINESH , M M THWIN , et al . RANKL targeted peptides inhibit osteoclastogenesis and attenuate adjuvant induced arthritis by inhibiting NF- κ B activation and down regulating inflammatory cytokines [J]. Chemico Biol Interact , 2013 , 203 ( 2 ): 467 - 479 .

何莲花，李逸群，王靖霞，等．风湿祛痛胶囊对TNF- α 诱导的人类风湿关节炎成纤维样滑膜细胞功能的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 7 ): 116 - 121 .

张靖，周彬，王彦丽，等 . 抗类风湿性关节炎中药的研究进展 [J]. 中草药， 2013 , 44 ( 15 ): 2189 - 2194 .

王志强，宫彩霞，李振彬．基于信号通路的中药有效成分治疗类风湿关节炎机制研究进展 [J]．中国实验方剂学杂志， 2019 ， 25 ( 8 ): 226 - 234 .

苏杉，王蓉，赵敏，等．秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子- κ B的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 19 ): 159 - 165 .

杜成成，谭余庆，沈建英，等．双氢青蒿素对两种类风湿关节炎模型的影响及机制 [J]．中国实验方剂学杂志， 2019 ， 25 ( 10 ): 48 - 56 .

S MATEEN , A ZAFAR , S MOIN , et al . Understanding the role of cytokines in the pathogenesis of rheumatoid arthritis [J]. Clin Chimi Acta , 2016 ， 455 : 161 - 171 .

S DADOUN , N ZEBOULON-KTORZA , C COMBESCURE , et al . Mortality in rheumatoid arthritis over the last fifty years: systematic review and meta-analysis [J]. Joint Bone Spine , 2013 ， 80 ( 1 ): 29 - 33 .

G S FIRESTEIN , I B MCINNES . Immunopathogenesis of rheumatoid arthritis [J]. Immunity , 2017 , 46 : 183 - 196 .

K P LIAO . Cardiovascular disease in patients with rheumatoid arthritis [J]. Trends Cardiovasc Med , 2017 ， 27 : 136 - 140 .

L A RIDGLEY , A E ANDERSON , A G PRATT . What are the dominant cytokines in early rheumatoid arthritis？ [J]． Curr Opin Rheumatol , 2018 ， 30 : 207 - 214 .

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