Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-<italic style="font-style: italic">κ</italic>B Signal Pathway

Yue ZHOU; Hang ZHONG; Cheng-peng LI; Bing LIU

doi:10.13422/j.cnki.syfjx.20200404

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Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-κB Signal Pathway

Classic Prescriptions | 更新时间：2021-02-09

- Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-κB Signal Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 39-45(2020)
- 作者机构：
  
  贵州大学　药学院，贵阳　 550025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200404
  CLC： R2-0; R22; R285.5
- Received：24 July 2019，
  
  Published Online：06 November 2019，
  
  Published：20 March 2020
- 稿件说明：
移动端阅览
Yue ZHOU, Hang ZHONG, Cheng-peng LI, et al. Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-κB Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 39-45.
DOI：

Yue ZHOU, Hang ZHONG, Cheng-peng LI, et al. Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-κB Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 39-45. DOI： 10.13422/j.cnki.syfjx.20200404.

摘要

目的：

基于核转录因子-

B(NF-

B)信号通路探究活血定痛方对心肌缺血再灌注损伤动物模型的保护作用。

方法：

SD大鼠随机分为假手术组、模型组、复方丹参滴丸组，活血定痛高、低剂量组，活血定痛高剂量＋NF-

B抑制剂组，各组动物连续给药2周，活血定痛高剂量组＋二硫代氨基甲酸吡咯烷(PDTC)组于造模后次日腹腔注射PDTC；结扎左冠状动脉前降支造模，检测左心功能及Na

＋

-K

＋

-ATP酶。各组动物腹主动脉取血，酶联免疫吸附测定(ELISA)法检测血清中肌酸激酶同工酶(CK-MB)，肌钙蛋白T(cTnT)，肿瘤坏死因子-

(TNF-

)，白细胞介素-6(IL-6)，白细胞介素-1

(IL-1

)，超氧化物歧化酶(SOD)，丙二醛(MDA)，谷胱甘肽过氧化物酶(GSH-Px)。蛋白免疫印迹法(Western blot)检测心肌NF-

B，NF-

B抑制蛋白

)，NF-

B抑制蛋白激酶(I

κκ

B)的蛋白表达。

结果：

与模型组比较，复方丹参滴丸组和活血定痛高、低剂量组可不同程度降低血清中CK-MB，cTnT，TNF-

，IL-6，IL-1

，MDA的水平，增加SOD，GSH-Px水平，上调心肌组织I

κκ

水平激酶I

和I

κκ

B的蛋白表达水平，提高心肌缺血再灌注模型动物心肌能量代谢过程中的Na

＋

-K

＋

-ATP酶活性(

0.05)；而活血定痛高剂量组＋PDTC并未降低血清中CK-MB，cTnT，TNF-

，IL-6，IL-1

，MDA的含量，增加SOD，GSH-Px水平，上调心肌组织I

κκ

水平激酶I

和I

κκ

B的蛋白表达水平，与模型组比较差异无统计学意义。

结论：

活血定痛方可通过上调心肌缺血再灌注模型心肌组织中I

和I

κκ

B的蛋白表达，抑制NF-

B信号通路的活化，降低炎症介质表达及自由基产生，提高心肌能量代谢过程中的Na

＋

-K

＋

-ATP酶活性，达到较好的抗心肌缺血再灌注损伤的作用。

Abstract

Objective:

To explore the protective effect of Huoxue Dingtong prescription on myocardial ischemia reperfusion injury in animal model based on nuclear transcription factor-

B(NF-

B) signal pathway.

Method:

In compound Danshen dropping pill group

SD rats were randomly divided into sham group

model group

compound salvia miltiorrhiza dropping pill group

high-dose Huoxue Dingtong group low-dose Huoxue Dingtong group

high-dose Huoxue Dingtong+ NF-

B inhibitor group. The rats in each group were administered continuously for 2 weeks. The rats in high-dose Huoxue Dingtong group+ pyrrolidine dithiocarbamate(PDTC) group were intraperitoneally administered the next day after modeling. Injection with PDTC and ligation of anterior descending branch of left coronary artery were performed to detect left ventricular function and Na

-K

-ATPase activity. Blood was collected from each animal abdominal aorta

and enzyme-linked immunosorbent assay (ELISA) was used to detect serum creatine kinase isoenzyme (CK-MB)

troponin T (cTnT)

tumor necrosis factor-

(TNF-

)

interleukin-6 (IL-6)

interleukin-1

(IL-1

)

superoxide gasification enzyme (SOD)

malondialdehyde (MDA)

glutathione peroxidase (GSH-Px). Western blot was used to detect the expressions of NF-

NF-

B inhibitor

) and I

B kinase(I

κκ

B) in myocardium.

Result:

Compared with model group

compound Danshen dropping pill group

high-dose Huoxue Dingtong group and low-dose Huoxue Dingtong group could reduce serum CK-MB

cTnT

TNF-

IL-6

IL-1

MDA

increase SOD and GSH-Px contents

increase the protein expressions of I

and I

B in myocardial tissue

and increase the activity of Na

-K

-ATPase in myocardial energy metabolism in myocardial ischemia-reperfusion model rats (

0.05). However

high-dose Huoxue Dingtong group+ PDTC did not decrease serum CK-MB

cTnT

TNF-

IL-6

IL-1

and MDA

increase SOD

GSH-Px

and increase the protein expression levels of I

and I

κκ

B in myocardial tissue. There was no significant difference between high-dose Huoxue Dingtong group+ PDTC and model group.

Conclusion:

Huoxue Dingtang prescription can inhibit the activation of NF-

B signaling pathway

reduce the expression of inflammatory mediators and the production of free radicals

and increase the activity of Na

-K

-ATPase in the process of myocardial energy metabolism by up-regulating the expressions of I

and I

κκB

proteins in myocardial tissue of myocardial ischemia-reperfusion model.

关键词

Keywords

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⁰

Protective Effect of Huoxue Dingtong Prescription on Myocardial Ischemia Reperfusion Injury in Animal Model Based on NF-κB Signal Pathway

DOI：10.13422/j.cnki.syfjx.20200404

摘要

Abstract

关键词

Keywords

references