Grey Correlation Analysis on Rate of Embryo, Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds

Shi-peng FU; Hong-wei SHEN; Qian-bo WANG; Jun-ping LI; Cong WANG; Shuang ZHANG; Yue LIU; Sheng-lei GUO; Zhen-yue WANG

doi:10.13422/j.cnki.syfjx.20200411

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Grey Correlation Analysis on Rate of Embryo, Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds

Topic on Basic Research of Ecological Planting of Acanthopanax senticosus | 更新时间：2021-02-09

- Grey Correlation Analysis on Rate of Embryo, Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 4, Pages: 145-151(2020)
- 作者机构：
  
  1.黑龙江中医药大学，哈尔滨　 150040
  2.江阴天江药业有限公司，江苏江阴　 214400
  3.广东药科大学　附属第一医院，广州　 510000
  4.黑龙江珍宝岛药业博士后科研工作站，哈尔滨　 150040
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200411
  CLC： R284.2; R289; R22; R2-031
- Received：22 April 2019，
  
  Published Online：07 November 2019，
  
  Published：20 February 2020
- 稿件说明：
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Shi-peng FU, Hong-wei SHEN, Qian-bo WANG, et al. Grey Correlation Analysis on Rate of Embryo, Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 145-151.
DOI：

Shi-peng FU, Hong-wei SHEN, Qian-bo WANG, et al. Grey Correlation Analysis on Rate of Embryo, Endogenous Hormone and Enzyme Activity of Acanthopanax senticosus Seeds[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(4): 145-151. DOI： 10.13422/j.cnki.syfjx.20200411.

摘要

目的：

研究刺五加种子在层积过程中的内源激素含量、相关酶活力与胚率的相关性研究，为生产和科研中应用内源激素和相关生理指标来调控刺五加种子萌发提供理论依据。

方法：

采用刺五加内生真菌，不同浓度的一氧化氮和过氧化氢溶液进行浸种处理后对刺五加种子进行变温层积处理，利用高效液相色谱法测定刺五加种子中内源激素[赤霉素(GA

)，脱落酸(ABA)，吲哚乙酸(IAA)，吲哚丁酸(IBA)及水杨酸(SA)]的含量，并且检测体内酶[过氧化氢酶(CAT)，超氧化物歧化酶(SOD)，过氧化物酶(POD)，丙二醛(MDA)]

活力变化。通过灰色关联度法探讨胚率和刺五加种子中激素和其酶活力的相关性。

结果：

胚率与IAA，GA

，CAT有显著正相关联，关联系数分别为1.086，0.935，1.067，IAA，GA

，CAT，IBA，SA之间存在显著的正相关性，与ABA，MDA，POD之间具有明显的负相关性，SOD的活性与其他含量之间无显著的相关性。关联度大小比较为IAA

CAT

IBA

SOD

ABA

POD

MDA。

结论：

IAA，GA

，CAT对胚率有明显促进作用，激素含量与酶活力之间有明显的相关性，可为探讨刺五加种子萌发机制提供理论依据。

Abstract

Objective:

To study the correlation between endogenous hormone content

related enzyme activity and embryogenesis during the stratification of

Acanthopanax senticosus

seeds

and to provide theoretical basis for application of endogenous hormones and related physiological indicators in regulating germination of

senticosus

seeds in production and scientific research.

Method:

Endophytic fungi as well as different concentrations of nitric oxide and hydrogen peroxide solution were used for soaking the seeds of

senticosus

and then thermophilic stratification was conducted for the seeds. The content of endogenous hormones such as gibberellin (GA

)

abscisic acid (ABA)

indole acetic acid (IAA)

indole butyric acid (IBA) and salicylic acid (SA) in seeds of

senticosus

were tested by high performance liquid chromatography (HPLC)

and the activity change of enzymes

in vivo

such as catalase (CAT)

superoxide dismutase (SOD)

peroxidase (POD)

and malondialdehyde (MDA) were tested. The correlation between the embryo rate and the hormones and their enzyme activities in the seeds of

senticosus

was studied by the grey correlation method.

Result:

The embryo rate was significantly positively correlated with IAA

and CAT

with correlation coefficient of 1.086

0.935 and 1.067

respectively. There was a significant positive correlation between IAA

CAT

IBA

and SA

but with a significant negative correlation with ABA

MDA

and POD. The correlation degree was as follows: IAA

CAT

IBA

SOD

ABA

POD

MDA.

Conclusion:

IAA

and CAT have significant promoting effects on embryo rate

and there is a significant correlation between hormone content and enzyme activity

which provides a basis for exploring the seed germination mechanism of

senticosus

关键词

Keywords

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