Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells

Xiao-lan JIAN; Pu-hua ZENG; Yong-min LI; Xiao-ning TAN; Feng-jiao HE; Yi-lan JIANG

doi:10.13422/j.cnki.syfjx.20200422

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Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells

Pharmacology | 更新时间：2021-02-09

- Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 7, Pages: 73-78(2020)
- 作者机构：
  
  湖南省中医药研究院　附属医院，中医肿瘤学湖南省重点实验室，抗肿瘤中药创新平台，长沙　 410006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200422
  CLC： R22; R242; R2-031;R285.5
- Received：28 June 2019，
  
  Published Online：04 November 2019，
  
  Published：05 April 2020
- 稿件说明：
移动端阅览
Xiao-lan JIAN, Pu-hua ZENG, Yong-min LI, et al. Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 73-78.
DOI：

Xiao-lan JIAN, Pu-hua ZENG, Yong-min LI, et al. Effect of Jianpi Xiao' ai Prescription on Akt/mTOR Signaling Pathways in Colorectal Cells[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 73-78. DOI： 10.13422/j.cnki.syfjx.20200422.

摘要

目的：

探讨健脾消癌方含药血清对人结肠癌HCT116细胞蛋白激酶B/哺乳动物雷帕霉素靶蛋白(Akt/mTOR)信号通路相关蛋白表达的影响。

方法：

以15%健脾消癌方含药血清处理结肠癌HCT116细胞，采用Transwell侵袭实验检测HCT116细胞迁移、侵袭作用，采用蛋白质印迹法(Western blot)检测HCT116细胞中Akt，磷酸化蛋白激酶B(p-Akt)，mTOR，磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)，核糖体S6蛋白激酶(S6K1)，磷酸化核糖体S6蛋白激酶(p-S6K1)，真核细胞始动因子4E结合蛋白(4EBP1)，磷酸化真核细胞始动因子4E结合蛋白(p-4EBP1)等蛋白的表达，正常血清组设立相同浓度正常血清组(15%)，10%胎牛血清组(FBS)。

结果：

与正常血清组比较，健脾消癌方含药血清组迁移细胞数与侵袭细胞数均显著降低(

0.01)；与正常血清组比较，Akt蛋白表达无明显下调，p-Akt蛋白表达显著下调(

0.01)；与正常血清组比较，mTOR蛋白表达无明显下调，p-mTOR蛋白表达显著下调(

0.01)；与正常血清组比较，S6K1蛋白表达无明显下调，p-S6K1蛋白表达显著下调(

0.01)；与正常血清组比较，4EBP1蛋白表达无明显下调，p-4EBP1蛋白表达显著下调(

0.01)。

结论：

健脾消癌方抗结肠癌复发转移的机制可能与抑制Akt/mTOR信号通路的激活有关。

Abstract

Objective:

To investigate the effect of drug-containing serum of Jianpi Xiaoai prescription on protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathways in colorectal cells HCT116.

Method:

The HTC116 cells were treated by 15%concentration of drug-contained serum

and then the cell migration and invasion were detected by Transwell assay

the protein expression levels of Akt

phosphorylated protein kinase B (p-Akt)

mTOR

phosphorylated mammalian target of rapamycin (p-mTOR)

ribosomal protein S6 kinase

polypeptide1(S6K1)

phosphorylated ribosomal protein S6 kinase

polypeptide1 (p-S6K1)

4E-binding protein1(4EBP1)

and phosphorylated 4E-binding protein1(p-4EBP1) in HCT116 cells were detected by Western blot. The control group was treated by untreated serum (15%)

and 10%fetal bovine serum(FBS).

Result:

As compared with the control group

the number of migration and invasion cells was significantly reduced in drug-contained serum group (

0.01)

the expression of Akt had no obvious decrease

p-Akt protein expression was significantly lowered in the drug-contained serum group (

0.01)

the expression of mTOR had no obvious decrease

but p-mTOR protein expression was significantly lowered in drug-contained serum group (

0.01)

the expression of S6K1 had no obvious decrease

but p-S6K1 protein expression was significantly lowered in the drug-contained serum group (

0.01)

the protein expression of 4EBP1 had no obvious decrease

but p-4EBP1 protein expression was significantly lowered in the drug-contained serum group (

0.01).

Conclusion:

The anti-tumor mechanism and transfer of Jianpi Xiaoai prescription may be related to inhibiting the activation of Akt/mTOR signaling pathways in colorectal cancer.

关键词

Keywords

references

李道娟，李倩，贺宇彤．结直肠癌流行病学趋势 [J]．肿瘤防治研究， 2015 , 42 ( 3 ): 305 - 310 .

万德森．结直肠癌流行病学与预防 [J]．中国中西医结合外科杂志， 2011 , 17 ( 1 ): 3 - 7 .

陈万青，郑荣寿，张思维，等 . 2013年中国恶性肿瘤发病和死亡分析 [J]．中国肿瘤， 2017 , 26 ( 1 ): 1 - 7 .

崔晓茹，侯勇，于庆生．中医药辅助治疗结直肠癌研究进展 [J]．安徽中医药大学学报， 2017 , 36 ( 2 ): 94 - 96 .

卢冬雪，刘沈林．中医药防治结直肠癌术后复发转移的概况 [J]．世界中西医结合杂志， 2017 ， 12 ( 17 ): 1620-1623，1628 .

陶丽，朱莹杰，顾缨，等．中医药辨证治疗对结直肠癌肝转移根治性切除的预后影响 [J]．中华中医药学刊， 2018 ， 36 ( 5 ): 1051 - 1056 .

蒋益兰，潘敏求，蔡美．健脾消癌饮配合化疗拮抗大肠癌术后复发转移62例总结 [J]．湖南中医杂志， 2007 ， 23 ( 1 ): 1 - 3 .

蒋益兰，潘博，仇湘中．健脾消癌饮配合化疗治疗大肠癌术后40例总结 [J]．湖南中医杂志， 2001 ， 17 ( 5 ): 9 - 10 .

简小兰，杨晓，罗吉，等．健脾益气化瘀解毒方含药血清对结肠癌HCT116细胞增殖、周期、凋亡的影响 [J]．北京中医药大学学报， 2016 ， 39 ( 11 ): 909 - 914 .

A BAHRAMI , M KHAZAEI , M HASANZADEH , et al . Therapeutic potential of targeting PI3K/Akt pathway in treatment of colorectal cancer: rational and progress [J]. J Cell Biochem , 2018 ， 119 ( 3 ): 2460 - 2469 .

贺石林，王键，王净净．中医科研设计与统计学 [M]．长沙：湖南科学技术出版社， 2012 ： 48 - 49 .

C BARTHOLOMEUSZ , A M GONZALEZ-ANGULO ． Targeting the PI3K signaling pathway in cancer therapy [J]. Expert Opin Ther Targets , 2012 , 16 ( 1 ): 121 - 130 .

K INOKI , M N CORRADETTI , K L GUAN ． Dysregulation of the TSC-mTOR pathway in human disease [J]. Nat Genet , 2004 , 37 ( 1 ): 19 - 24 .

N NATHAN , K M KEPPLER-NOREUIL , L G BIESECKER , et al . Mosaic disorders of the PI3K/PTEN/Akt/TSC/mTORC1 signaling pathway [J]. Diabetologia , 2008 ， 51 ( 3 ): 512 - 521 .

A DUFNER , G THOMAS . Ribosomal S6 kinase signaling and the control of translation [J]. Exp Cell Res , 1999 , 253 ( 1 ): 100 - 109 .

J YAN , H ZHOU , L KONG , et al . Identification of two novel inhibitors of mTOR signaling pathway based on high content screening [J]. Cancer Chemother Pharmacol , 2013 , 23 ( 10 ): 123 - 127 .

G JUHASZ , J H HILL , Y YAN , et al . The class Ⅲ PI(3) K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila [J]. J Cell Biol ， 2008 ， 181 ( 4 ): 655 - 666 .

G PAT , K A BOWEN , L JIANYU , et al . mTORC1 and mTORC2 regulate EMT, motility and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways [J]. Cancer Res , 2011 , 71 ( 9 ): 3246 - 3256 .

闫克敏，肖海娟，杨林，等，人参皂苷Rh 2 对结肠癌耐药细胞HCT116/L-OHP侵袭迁移能力的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 13 ): 73 - 78 .

刘帆，侯林，张晓平，等，薏苡仁多糖抗肿瘤作用及免疫作用研究进展 [J]．辽宁中医药大学学报， 2019 ， 21 ( 3 ): 123 - 126 .

田新宇，范翠梅，渠田田，等．半枝莲总黄酮中7种成分的含量测定及抗肿瘤活性 [J]．中国实验方剂学杂志， 2017 ， 23 ( 1 ): 53 - 59 .

朱燕，谢丽，杨觅，等．重楼抗肿瘤作用机制的研究进展 [J]．癌症进展， 2015 ， 13 ( 2 ): 164 - 166 .

孙学然，杨克，吕玲玲，等．莪术二酮对乳腺癌HCC1937细胞迁移和侵袭的影响及机制 [J]．中国实验方剂学杂志， 2019 ， 25 ( 3 ): 66 - 73 .

金海峰，王楼蕾，吕宾．郁金抗肿瘤作用的研究进展 [J]．医药导报， 2009 ， 28 ( 12 ): 1565 - 1568 .

何兰，简小兰，蒋益兰，等．健脾消癌方及拆方对结肠癌原位种植瘤裸鼠模型钙黏蛋白、波形蛋白表达的影响 [J]．中华中医药学刊， 2018 ， 36 ( 6 ): 1324 - 1327 .

邢作英，王永霞，曹英杰，等．血清药理学研究概要 [J]．世界科学技术—中医药现代化， 2015 ， 17 ( 1 ): 219 - 222 .

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