Study on Inhibitory Effect of Volatile Oils in 16 Plants on <italic style="font-style: italic">Aspergillus flavus</italic> of Platycladi Semen

Ting LI; Dan JIANG; Xiao-xi CHANG; Guo-dong HUA; Chun-sheng LIU

doi:10.13422/j.cnki.syfjx.20200447

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Study on Inhibitory Effect of Volatile Oils in 16 Plants on Aspergillus flavus of Platycladi Semen

Pharmacy Fundamentals | 更新时间：2021-02-09

- Study on Inhibitory Effect of Volatile Oils in 16 Plants on Aspergillus flavus of Platycladi Semen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 132-136(2020)
- 作者机构：
  
  1.北京中医药大学　中药学院，北京　 102488
  2.北京中医药大学　东直门医院，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200447
  CLC： R22; R931; R282; S895.1+ 36
- Received：16 September 2019，
  
  Published Online：06 November 2019，
  
  Published：05 March 2020
- 稿件说明：
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Ting LI, Dan JIANG, Xiao-xi CHANG, et al. Study on Inhibitory Effect of Volatile Oils in 16 Plants on Aspergillus flavus of Platycladi Semen[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 132-136.
DOI：

Ting LI, Dan JIANG, Xiao-xi CHANG, et al. Study on Inhibitory Effect of Volatile Oils in 16 Plants on Aspergillus flavus of Platycladi Semen[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 132-136. DOI： 10.13422/j.cnki.syfjx.20200447.

摘要

目的：

从16种植物(香茅、香叶、松节、山苍子、薄荷、干姜、丁香、姜黄、红花椒、肉桂、罗勒、迷迭香、青花椒、竹叶花椒、八角茴香、肉豆蔻)挥发油中筛选出对黄曲霉菌生长有较好抑制效果的植物挥发油。

方法：

采用平板培养法从柏子仁药材表面分离得到黄曲霉菌，利用水蒸气蒸馏法提取16种植物挥发油，采用滤纸片熏蒸后测定黄曲霉菌的菌落直径，对16种植物挥发油抑制黄曲霉菌生长的效果进行研究。

结果：

采用性状、显微及DNA条形码鉴定的方法从柏子仁药材上成功分离了黄曲霉菌，上述16种植物挥发油对黄曲霉菌的抑菌率分别为2.93%，0.05%，0.37%，76.07%，0.34%，0.15%，50.05%，8.51%，1.43%，58.20%，0.07%，2.60%，8.73%，100.00%，52.62%，0.07%。

结论：

16种植物挥发油对黄曲霉菌均有着不同程度的抑菌活性，其中竹叶花椒挥发油、山苍子挥发油、肉桂挥发油的抑菌效果较好，可为柏子仁生长储藏过程中防治黄曲霉菌的污染提供参考，并为植物挥发油作为中药仓储过程中的抑菌剂提供应用依据。

Abstract

Objective:

To select volatile oils from 16 species of plants (

Cymbopogon citratus

Pelargonium graveolens

Pinus tabulieformis

Litsea cubeba

Mentha haplocalyx

Zingiber officinale

Syzygium aromaticum

Curcuma longa

Zanthoxylum bungeanum

Cinnamomum cassia

Ocimum basilicum

Rosmarinus officinalis

Zanthoxylum schinifolium

Zanthoxylum armatum

Illicium verum

Myristica fragrans

) that have good inhibitory effect on the growth of

Aspergillus flavus

Method:

Aspergillus flavus

was isolated from the surface of Platycladi Semen medicinal materials by plate culture method. The volatile oils of 16 plants were extracted by steam distillation. The colony diameter of

Aspergillus flavus

was determined by fumigation of filter paper

and the effect of volatile oils on the growth of

Aspergillus flavus

was systematically studied.

Result:

Aspergillus flavus

was successfully isolated from Platycladi Semen by means of morphological

microscopic and DNA barcoding identification methods

the bacteriostatic rates of the above 16 kinds of volatile oils against

Aspergillus flavus

were 2.93%

0.05%

0.37%

76.07%

0.34%

0.15%

50.05%

8.51%

1.43%

58.20%

0.07%

2.60%

8.73%

100.00%

52.62%

0.07%

respectively.

Conclusion:

The volatile oils of 16 plants all have different degrees of antibacterial activities for

Aspergillus flavus

and volatile oils of

Zanthoxylum armatum

Litsea cubeba

and

Cinnamomum cassia

have good inhibitory effect. This study can provide a theoretical basis for the prevention and control of

Aspergillus flavus

in the growth and storage of Platycladi Semen

and provide a basis for further research on plant volatile oil as bacteriostatic agents in the storage process of traditional Chinese medicine.

关键词

Keywords

references

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Related Institution

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Study on Inhibitory Effect of Volatile Oils in 16 Plants on Aspergillus flavus of Platycladi Semen

DOI：10.13422/j.cnki.syfjx.20200447

摘要

Abstract

关键词

Keywords

references