Ater-soluble Protein Profiles of <italic style="font-style: italic">Astragalus membranaceus</italic> var. <italic style="font-style: italic">mongholicus</italic> and A. <italic style="font-style: italic">membranaceus</italic>

Ying-li ZHAO; Juan LI; Xing FANG; Bei-bei GAO; Ting-fei TAN; Peng GONG; Quan XIA

doi:10.13422/j.cnki.syfjx.20200512

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Ater-soluble Protein Profiles of Astragalus membranaceus var. mongholicus and A. membranaceus

Pharmacy Fundamentals | 更新时间：2021-02-09

- Ater-soluble Protein Profiles of Astragalus membranaceus var. mongholicus and A. membranaceus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 137-144(2020)
- 作者机构：
  
  1.合肥市第二人民医院，合肥　 230011
  2.安徽医科大学　第一附属医院，合肥　 230022
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200512
  CLC： R284.2; R289; R22; R2-031
- Received：12 July 2019，
  
  Published Online：27 November 2019，
  
  Published：05 March 2020
- 稿件说明：
移动端阅览
Ying-li ZHAO, Juan LI, Xing FANG, et al. Ater-soluble Protein Profiles of Astragalus membranaceus var. mongholicus and A. membranaceus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 137-144.
DOI：

Ying-li ZHAO, Juan LI, Xing FANG, et al. Ater-soluble Protein Profiles of Astragalus membranaceus var. mongholicus and A. membranaceus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 137-144. DOI： 10.13422/j.cnki.syfjx.20200512.

摘要

目的：

蒙古黄芪和膜荚黄芪都为药用黄芪，二者亲缘关系近，成分相似，药用价值尚未区分。该研究旨在建立蒙古黄芪和膜荚黄芪的水溶性蛋白表达谱，了解两种黄芪之间存在的差别。

方法：

样品采用水超声提取和丙酮沉淀的方法得到黄芪水溶性蛋白成分，质谱级胰酶酶解后的肽段经nano ESI-LC-MS/MS分析，采用Proteome Discoverer 1.4软件比对豆科蛋白数据库鉴定蛋白，再使用非标记定量软件SIEVE分析蒙古黄芪与膜荚黄芪水溶性蛋白质表达的差异。最后运用生物信息学方法分析2种黄芪共有蛋白的分类、分子功能、参与的生物过程和信号通路。

结果：

蒙古黄芪鉴定特异性蛋白有920个，膜荚黄芪鉴定的特异性蛋白有717个，2种黄芪中共同表达的蛋白有472个，其中二者的差异表达蛋白有21个，如PR-10蛋白，NDK-1蛋白，谷蛋白A

，磷脂酶D等蛋白在两种黄芪中差异表达。蒙古黄芪高表达蛋白14个，膜荚黄芪高表达蛋白7个。

结论：

蒙古黄芪和膜荚黄芪的水溶性蛋白表达谱存在显著差异，特异性蛋白、差异表达蛋白和共同表达的蛋白可为今后两种黄芪的鉴别、药理作用机制的研究和寻找作用靶点提供参考。

Abstract

Objective:

Astragalus membranaceus

var.

mongholicus

and

membranaceus

are medicinal

Astragalus

which are closely related and similar in composition

but with unclear medicinal value. Water-soluble protein profiles for

membranaceus

var.

mongholicus

and

membranaceus

were established to explore the differences between the two kinds of Astragalus Radix.

Method:

The water-soluble protein components were obtained through water ultrasonic extraction and acetone precipitation. After digested with trypsin

the obtained peptides were analyzed by nano ESI-LC-MS/MS method. Proteome Discovery 1.4 software was used to identify the proteins by comparing with the legume protein database

and the different expression water-soluble proteins were analyzed by the label-free quantitative software SIEVE. Finally

relevant information for common expression proteins

including classification

molecular function

involved biological process and signaling pathway

were analyzed by bioinformatics.

Result:

There were 920 and 717 specific proteins identified for

membranaceus

var.

mongholicus

and

membranaceus

respectively. Totally 472 proteins were found to be co-expressed

in which 21 were differentially expressed

such as PR-10 protein

NDK-1 protein

glutelin A

and phospholipase D. There were 14 highly expressed proteins in

membranaceus

var.

mongholicus

and 7 highly expressed proteins in

membranaceus

Conclusion:

There are significant differences in water-soluble protein profiles for two kinds of Astragalus Radix. Specific proteins

differentially expressed proteins and common expressed proteins can provide references for the identification of

membranaceus

var.

mongholicus

and

membranaceus

. It also can be used to define pharmacological mechanisms and search for drug action targets.

关键词

Keywords

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Ater-soluble Protein Profiles of Astragalus membranaceus var. mongholicus and A. membranaceus

DOI：10.13422/j.cnki.syfjx.20200512

摘要

Abstract

关键词

Keywords

references