Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines

Yu WANG; Xue YANG; Peng-fei XIA; Rui-jing YANG; Yu-xia WANG; Fei-xia YANG; Qin FAN; Xiao-yu WU; Xue-jing PENG; Lei ZHAO

doi:10.13422/j.cnki.syfjx.20200513

您当前的位置：

首页 >

文章列表页 >

Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines

Academic Discussion | 更新时间：2021-02-09

- Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 241-250(2020)
- 作者机构：
  
  1.甘肃中医药大学　药学院，兰州　 730000
  2.北京中医药大学　药学院，北京　 102488
  3.甘肃中医药大学　甘肃省高校中藏药化学与质量研究省级重点实验室，兰州　 730000
  4.甘肃省道地药材质量标准化技术研究与推广工程实验室，兰州　 730000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200513
  CLC： R284.2; R289; R22; R2-031
- Received：14 June 2019，
  
  Published Online：27 November 2019，
  
  Published：20 April 2020
- 稿件说明：
移动端阅览
Yu WANG, Xue YANG, Peng-fei XIA, et al. Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 241-250.
DOI：

Yu WANG, Xue YANG, Peng-fei XIA, et al. Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 241-250. DOI： 10.13422/j.cnki.syfjx.20200513.

摘要

由于中药作用的整体性、成分和作用机制的复杂性，化学成分(群)作为中药及其复方制剂发挥治疗作用的物质基础，因此，采用多种方法分离分析中药及其复方中的化学成分一直是中药治病防病研究中一个有待解决的关键重要问题。传统方法存在操作繁琐、耗时耗事、浪费溶剂和样品的不可逆吸附等缺陷，并且分离过程中无法给出药理参数的基本信息，因而无法对活性成分进行高效准确定位。近年来从细胞层次研究药物成分与受体的结合为中药活性成分的筛选研究提供了一种新的途径。目前研究二者相互作用的方法之一—细胞膜色谱法(CMC)，将现有色谱及质谱技术、细胞生物学与受体药理学结合起来，能够正确反映活性部位、活性成分与细胞膜及膜受体的相互作用，对复杂中药体系中有效部位筛选、活性成分的分离和高通量筛选具有独特优势。该文综述了CMC的原理、特点、中药活性成分筛选领域中的细胞膜模型及其与气相色谱-质谱(GC-MS)及液相色谱-质谱(LC-MS)联用的研究现状，并对其今后研究方法和未来的开发前景进行了探讨，为CMC在中医药领域的深入研究和开发利用提供理论依据和实践指导。

Abstract

The chemical components (groups) contained in traditional Chinese medicine(TCM) and its compound preparations are the material basis for its curative effect

because of the integrity of the action of TCM and the complexity of its compositions and mechanism. The separation and analysis of chemical constituents in TCM and its compound prescriptions by various methods is always a key problem to be solved in the research of disease prevention and treatment of TCM. The binding of drug molecules to receptors at the cellular level was explored to provide a new idea for the screening of active components of TCM or its compound preparations. Traditional methods have some drawbacks

such as cumbersome operation

time-consuming

waste of solvents and irreversible adsorption of samples. The basic information of pharmacological parameters cannot be given in the separation process

and the active ingredients cannot be efficiently and accurately located. At present

cell membrane chromatography (CMC)

one of the methods

is used to study the interaction between drug molecules and receptors

and can combine the existing chromatography and mass spectrometry technology

cell biology and receptor pharmacology

and correctly reflect the interaction between active parts

active components and cell membrane and membrane receptors

so it has unique advantages in screening effective parts

separation of active components and high-throughput screening from complex TCM system. The principles and characteristics of CMC

the cell membrane model in the field of active ingredient selection of TCM and its research status in combination with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were reviewed

and its development prospects and future research methods were discussed

which provides theoretical basis and practical guidance for the research and utilization of CMC in the field of TCM.

关键词

Keywords

references

D WANG , X X DU . Interpretation of adverse drug reaction reporting and monitoring and significance of traditional Chinese medicine adverse drug reaction monitoring [J]. Chin J Chin Mater Med ， 2012 ， 37 ( 18 )： 2686 - 2688 .

王新，薛晖，岳媛，等．高表达 β 1 肾上腺素受体细胞膜色谱法靶向筛选夏天无中活性成分 [J]．中国实验方剂学杂志， 2015 ， 21 ( 18 )： 65 - 68 .

陈芳有，罗永明，吴样明，等．亲和色谱技术在天然药物研究中的应用 [J]．中国实验方剂学杂志， 2014 ， 20 ( 11 )： 230 - 234 .

S L HAN , P ZHANG , F WEI , et al . Screening active components acting on α 1A adrenergic receptors from agrimony using a Sprague-Dawley rat prostate cell membrane chromatography online coupled HPLC/MS method [J]． Anal Methods ， 2012 ， 4 ( 10 )： 3351 - 3357 .

X X YANG , Y L ZHANG , X X ZHANG , et al . Cell membrane chromatography and its application in the analysis of bioactive ingredients of TCMs [J]． J Pharm Sci ， 2011 ， 20 ( 1 )： 20 - 25 .

王丽莉，张铁军．细胞膜色谱法及其在中药活性成分研究中的应用 [J]．药物评价研究， 2011 ， 34 ( 2 )： 110 - 114 .

付锦楠，张文萌，孙立新，等．中药地黄中抗化学性肝损伤的活性成分研究 [J]．江西科学， 2014 ， 32 ( 3 )： 295 - 300 .

洪敏，马宏宇，朱荃．肝细胞萃取-HPLC分析法的建立及其在栀子保肝效应物质初步分析中的应用 [J]．中国中药杂志， 2009 ， 34 ( 4 )： 450 - 453 .

徐文博，陈艺丹，周律，等．肝细胞亲和-HPLC法体外筛选三七总皂苷(PNS)抗肝细胞脂肪变性的活性成分 [J]．广东药科大学学报， 2017 ， 33 ( 2 )： 226 - 230 .

华永庆，洪敏，陈文星，等．茵陈蒿汤肝细胞结合成分对肿瘤细胞的影响 [J]．中药药理与临床， 2010 ， 26 ( 6 )： 1 - 2 .

X ZHANG , L W QI , L YI , et al . Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography [J]． Biomed Chromatogr ， 2008 ， 22 ( 2 )： 157 - 163 .

M J ZHANG , Y ZHOU , L CHEN , et al . An overview of potential molecular mechanisms involved in VSMC phenotypic modulation [J]． Histochem Cell Biol ， 2016 ， 145 ( 2 )： 119 - 130 .

X X YANG , Y W WANG , X X ZHANG , et al . Screening vasoconstriction inhibitors from traditional Chinese medicines using a vascular smooth muscle/cell membrane chromatography-offline-liquid chromatography-mass spectrometry [J]． J Sep Sci ， 2011 ， 34 ( 19 ): 2586 - 2593 .

代冬冬，侯晓芳，周明哲，等．血管平滑肌CMC-GC/MS联用方法的建立 [J]．西北大学学报：自然科学版， 2010 ， 40 ： 162 - 166 .

王艳微，畅瑞苗，王新，等．细胞膜色谱法与液相色谱-质谱联用筛选佛手的活性成分 [J]．中国中医药信息杂志， 2013 ， 20 ( 8 )： 66 - 68 .

吕艳妮，付佳，贺浪冲，等．细胞膜色谱在线HPLC-IT-TOF MS联用系统筛选分析射干中次野鸢尾黄素的抗EGFR活性作用 [J]．质谱学报， 2017 ， 38 ( 4 )： 425 - 432 .

王嗣岑，孙萌，张彦民，等．用高表达EGFR细胞膜色谱-HPLC/MS联用快速筛选独活中抗EGFR活性成分 [J]．中国科学：化学， 2010 ， 40 ( 6 )： 750 - 756 .

Y ZHAN , J LI , W MA , et al . Characterization of interactions between taspine derivate TPD7 and EGF receptor by cell membrane chromatography with zonal elution and frontal analysis [J]． Chromatographia ， 2016 ， 79 ( 23/24 )： 1585 - 1592 .

L YANG , M ZHU , Y KAN , et al . Investigation of the binding characteristics between ligands and epidermal growth factor receptor by cell membrane chromatography [J]． J Mol Recognit ， 2018 ， 31 ( 6 )： e2701 .

S L HAN , J HUANG , S C WANG , et al . Screening epidermal growth factor receptor antagonists from Radix et Rhizoma Asari by two-dimensional liquid chromatography [J]． J Sep Sci ， 2014 ， 37 ( 13 )： 1525 - 1532 .

X X YANG , R M CHANG , Y YUE , et al . Cardiac muscle/cell membrane chromatography-offline-liquid chromatography/mass spectrometry method to identify bioactive components from traditional Chinese medicines [J]． Anal Lett ， 2013 ， 46 ( 9 )： 1317 - 1330 .

岳宣峰，张延妮，张志琪，等．心肌细胞膜固定相色谱研究中药川芎提取液与受体的作用 [J]．中国中药杂志， 2005 ， 30 ( 2 )： 129 - 133 .

李明华．心肌细胞膜与中药朱砂七水溶性成分作用的色谱法研究 [D]．西安：陕西师范大学， 2008 .

C Q LI , L C HE . Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR4 receptor component from Atractylodes macrocephala Koidz [J]． Sci Chin Ser C: Life Sci ， 2006 ， 49 ( 2 )： 182 - 189 .

C Q LI , L C HE , H Y DONG , et al . Screening for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz [J]． J Ethnopharmacol ， 2007 ， 114 ( 2 )： 212 - 217 .

Y YUE , H XUE , X LI , et al . High-expression β 1 adrenergic receptor/cell membrane chromatography method based on a target receptor to screen active ingredients from traditional Chinese medicines [J]． J Sep Sci ， 2013 ， 37 ( 3 )： 244 - 249 .

H XUE , Y CHENG , X LI , et al . Rutaecarpine and evodiamine selected as β 1 -AR inhibitor candidates using β 1 -AR/CMC-offline-UPLC/MS prevent cardiac ischemia-reperfusion injury via energy modulation [J]． J Pharm Biomed Anal ， 2015 ， 115 ： 307 - 314 .

Y YUE , L DOU , X LI , et al . Screening β 1 AR inhibitors by cell membrane chromatography and offline UPLC/MS method for protecting myocardial ischemia [J]． J Pharm Biomed Anal ， 2015 ， 115 : 339 - 344 .

樊宏伟，余黎，洪敏，等．血小板细胞膜固相色谱法的建立及其对丹参效应物质的初步分析 [J]．中国药学杂志， 2004 ， 39 ( 5 )： 375 - 378 .

樊宏伟，朱荃，洪敏，等 . 血小板细胞膜固相色谱法在脉络宁注射液效应物质分析中的应用 [J]．中国药学杂志， 2006 ， 41 ( 1 )： 63 - 66 .

闫玉梅 . 血小板细胞膜色谱筛选模型的构建及P2Y-(12)受体的表达纯化研究 [D]．西安：西北大学， 2016 .

J WANG , Z HUANG , S LI , et al . Screening of anti-platelet aggregation agents from Panax notoginsengusing human platelet extraction and HPLC-DAD-ESI-MS/MS [J]． J Sep Sci ， 2008 ， 31 ( 6/7 )： 1173 - 1180 .

X CHEN , Y CAO , D LYU , et al . Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines [J]． J Chromatogr A ， 2012 ， 1242 ： 67 - 74 .

D JIA , X CHEN , Z ZHU , et al . On-line comprehensive two-dimensional HepG2 cell membrane chromatographic analysis system for charactering anti-hepatoma components from rat serum after oral administration of Radix Scutellariae：a strategy for rapid screening active compounds in vivo [J]． J Pharm Biomed Anal ， 2016 ， 118 ： 27 - 33 .

X DING , Y CAO , Y CHAI , et al . Development of APTES-decorated HepG2 cancer stem cell membrane chromatography for screening active components from Salvia miltiorrhiza [J]． Anal Chem ， 2016 ， 88 ( 24 )： 12081 - 12089 .

X HOU , S WANG , T ZHANG , et al . Recent advances in cell membrane chromatography for traditional Chinese medicines analysis [J]． J Pharmaceuti Biomed ， 2014 ， 101 : 141 - 150 .

F WEI , S WANG , X XIE , et al . Characterization the affinity of α 1A adrenoreceptor by cell membrane chromatography with frontal analysis and stoichiometric displacement model [J]． J Chromatogr B ， 2017 ， 1040 : 273 - 281 .

M SUN , W MA , L HE , et al . Simultaneous screening of four epidermal growth factor receptor antagonists from Curcuma longavia cell membrane chromatography online coupled with HPLC-MS [J]． J Sep Sci ， 2013 ， 36 ( 13 )： 2096 - 2103 .

Y GUO , S HAN , J J CAO , et al . Histamine H1 receptor cell membrane chromatography online high-performance liquid chromatography with mass spectrometry method reveals houttuyfonate as an activator of the histamine H1 receptor [J]． J Sep Sci ， 2014 ， 37 ( 21 )： 3188 - 3194 .

X WU , X CHEN , D JIA , et al . Characterization of anti-leukemia components from Indigo naturalis using comprehensive two-dimensional K562 /cell membrane chromatography and in silico target identification [J]． Sci Rep ， 2016 ， 6 ： 25491 .

F WEI , Q HU , J HUANG , et al . Screening active compounds from Corydalis yanhusuo by combining high expression VEGF receptor HEK293 cell membrane chromatography with HPLC-ESI-IT-TOF/MS n method [J]． J Pharm Biomed Anal ， 2017 ， 136 ： 134 - 139 .

S HAN , C LI , J HUANG , et al . Cell membrane chromatography coupled with UHPLC-ESI-MS/MS method to screen target components from Peucedanum praeruptorum Dunn acting on α 1A adrenergic receptor [J]． J Chromatogr B ， 2016 ， 1011 ： 158 - 162 .

J LIU , J YANG , S WANG , et al . Combining human periodontal ligament cell membrane chromatography with online HPLC/MS for screening osteoplastic active compounds from Coptidis Rhizoma [J]． J Chromatogr B ， 2012 ， 904 ： 115 - 120 .

D R Stoll , X P LI , X L WANG , et al . Fast comprehensive two-dimensional liquid chromatography [J]． J Chromatogr A ， 2007 ， 1168 ( 1/2 )： 3 - 43 .

W LI , X NIU , P ZHOU , et al . A combined peritoneal macrophage/cell membrane chromatography and offline GC-MS method for screening anti-inflammatory components from Chinese traditional medicine Houttuynia cordata Thunb [J]． Chromatographia ， 2011 ， 73 ( 7/8 )： 673 - 680 .

M LI , S WANG , L HE ． Development of an analytical method coupling cell membrane chromatography with gas chromatography-mass spectrometry via microextraction by packed sorbent and its application in the screening of volatile active compounds in natural products [J]． J Chromatogr B ， 2015 ， 974 ： 9 - 16 .

W F LI , X F NIU , L C HE , et al . A combined peritoneal macrophage/cell membrane chromatography and offline GC-MS method for screening anti-inflammatory components from Chinese traditional medicine Houttuynia cordata Thunb [J]． Chromatographia ， 2011 ， 73 ( 7/8 )： 673 - 680 .

Y ZHANG , F LIU , Q WU , et al . Recognition and identification of active components from Radix Bupleuri using human neuroblastoma SH-SY5Y cells [J]． Biomed Chromatogr ， 2016 ， 30 ( 3 )： 440 - 446 .

M J LIANG , L C HE , G D YANG , et al . Screening，analysis and in vitro vasodilatation of effective components from Ligusticum chuanxiong [J]． Life Sci ， 2005 ， 78 ( 2 )： 128 - 133 .

张宇，李婷，杨健，等．白芷中有效成分的筛选、分析及对大鼠血管活性的影响 [J]．西北药学杂志， 2015 ， 30 ( 1 )： 37 - 42 .

Y GUO , S HAN , J J CAO , et al . Histamine H 1 receptor cell membrane chromatography online high-performance liquid chromatography with mass spectrometry method reveals houttuyfonate as an activator of the histamine H 1 receptor [J]． J Sep Sci ， 2014 ， 37 ( 21 )： 3188 - 3194 .

郑华珠，王利胜，吴银爱，等． UPLC-MS/MS联合细胞膜固相色谱法分析补阳还五汤作用于神经元样PC12细胞的效应成分 [J]．中国实验方剂学杂志， 2018 ， 24 ( 4 ): 14 - 20 .

M LI , S C WANG , Y ZHANG , et al . An online coupled cell membrane chromatography with LC/MS method forscreening compounds from Aconitum carmichaeli Debx. acting on VEGFR-2 [J]． J Pharm Biomed Anal ， 2010 ， 53 ： 1063 - 1069 .

J H XIAO , H ZHANG ． An Escherichia coli cell membrane chromatography-offline LC-TOF-MS method for screening and identifying antimicrobial peptides from Jatropha curcas Meal protein isolate hydrolysates [J]． J Biomol Screen ， 2012 ， 17 ( 6 )： 752 - 760 .

J REN , D DAI , H DU , et al . Prostate cell membrane chromatography-LC/MS method for screening α 1A -adrenoceptor antagonists from Lotus Plumule [J]． Chromatographia ， 2011 ， 74 ( 5/6 )： 375 - 381 .

J HE , S HAN , F YANG , et al . Prostate cell membrane chromatography-liquid chromatography-mass spectrometry for screening of active constituents from Uncaria rhynchophylla [J]． J Chromatogr Sci ， 2013 ， 51 ( 10 )： 905 - 909 .

M SUN , J REN , L HE , et al . A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR [J]． J Chromatogr B ， 2010 ， 878 ( 28 )： 2712 - 2718 .

L WANG , J REN , S WANG , et al . A combined cell membrane chromatography and online HPLC/MS method for screening compounds from Radix Caulophylli acting on the human α 1A-adrenoceptor [J]． J Pharm Biomed Anal ， 2010 ， 51 ( 5 )： 1032 - 1036 .

WF LI , W XING , S WANG , et al . An online coupled peritoneal macrophage/cell membrane chromatography and highperformance liquid chromatography/mass spectrometry method to screen for anti-inflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei [J]． Biomed Chromatogr ， 2013 ， 27 ( 11 )： 1580 - 1586 .

S L HAN , T ZHANG , J HUANG , et al . New method of screening allergenic components from Shuanghuanglian injection：With RBL-2H3/CMC model online HPLC/MS system [J]． J Pharm Biomed Anal ， 2014 ， 88 ： 602 - 608 .

J LIU , J YANG , S C WANG , et al . Combining human periodontal ligament cell membrane chromatography with online HPLC/MS for screening osteoplastic active compounds from Coptidis Rhizoma [J]． J Chromatogr B ， 2012 ， 904 ： 115 - 120 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Screening of Active Compounds from Blume for Vascular Smooth Muscle Relaxation

Effective Components and Antiarrhythmic Mechanisms of Wenxin Granules Based on CMC/UPLC-Q-TOF/MS

Correlation of "Parts-components-properties" of Traditional Chinese Medicines from Latex-containing Plants

Screening Effective Sites of Momordicae Semen-Epimedii Folium and Anti-lung Cancer Mechanism of Its Prescription

Related Author

LI Xiu-fang

YANG Lian

ZHANG Wei-ming

LIN Qing

LI Guo-hua

WEI Wen-bin

DU Wuxun

LI Xiaofeng

Related Institution

The Second Affiliated Hospital of Tianjin University of TCM

Tianjin University of Traditional Chinese Medicine（TCM）

Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-yao

Henan Key Laboratory of Resource and Chemistry for Chinese Traditional Medicine

Henan Provincial Herbal Ecological Planting Engineering Technology Research Center

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰