Rapid Identification of Chemical Constituents of <italic style="font-style: italic">Pimpinella thellungiana</italic> by UHPLC-Q-Orbitrap HRMS

Rui LIU; Rui WANG; Ke PEI; Shu-rong ZHANG; Miao-rong PEI

doi:10.13422/j.cnki.syfjx.20200516

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Rapid Identification of Chemical Constituents of Pimpinella thellungiana by UHPLC-Q-Orbitrap HRMS

Pharmacy Fundamentals | 更新时间：2021-02-09

- Rapid Identification of Chemical Constituents of Pimpinella thellungiana by UHPLC-Q-Orbitrap HRMS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 145-151(2020)
- 作者机构：
  
  山西中医药大学　中药学院，山西晋中　 030619
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200516
  CLC： R284.2; R289; R22; R2-031
- Received：30 October 2019，
  
  Published Online：27 November 2019，
  
  Published：05 March 2020
- 稿件说明：
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Rui LIU, Rui WANG, Ke PEI, et al. Rapid Identification of Chemical Constituents of Pimpinella thellungiana by UHPLC-Q-Orbitrap HRMS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 145-151.
DOI：

Rui LIU, Rui WANG, Ke PEI, et al. Rapid Identification of Chemical Constituents of Pimpinella thellungiana by UHPLC-Q-Orbitrap HRMS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 145-151. DOI： 10.13422/j.cnki.syfjx.20200516.

摘要

目的：

应用超高液相色谱四级杆-静电场轨道肼高分辨质谱联用(UHPLC-Q-Orbitrap HRMS)技术，快速识别与鉴定羊红膻药材中主要化学成分。

方法：

采用Waters Acquity UPLC BEH C

色谱柱(2.1 mm×50 mm，1.7 μm)，流动相0.1%甲酸水溶液(A)-乙腈(B)进行梯度洗脱。质谱采用ESI源，负离子一级全扫描-数据依赖性二级质谱扫描(MS

)模式采集数据。通过高分辨质谱数据解析，并结合对照品数据和相关文献对羊红膻药材中的化学成分进行识别和鉴定。

结果：

通过精确相对分子质量和二级碎片离子分析，并结合对照品确认、质谱裂解规律和相关文献检索，共鉴定29个化合物，包括绿原酸类化合物19个，黄酮类7个，小分子有机酸3个，其中除木犀草素葡萄糖醛酸苷和芹菜素葡萄糖醛酸苷外，其余27个化合物为首次从该药材中发现。

结论：

建立的UHPLC-Q-Orbitrap HRMS可快速鉴定羊红膻药材中的化学成分，研究结果为其药效物质基础、质量控制以及临床应用等研究提供了科学的依据。

Abstract

Objective:

Ultra high performance liquid chromatography coupled with Q-Exactive Focus hybrid quadrupole and orbitrap high resolution mass spectrometer (UHPLC-Q-Orbitrap HRMS) was applied for identification of chemical constituents in

Pimpinella thellungiana

Method:

Chromatographic separation was performed on a WATERS BEH C

column (2.1 mm ×50 mm

1.7 μm). The mobile phase consisted of 0.1% formic acid aq (A) and acetonitrile (B) with a gradient elution. Mass spectral analysis were performed on Q-Exactive Focus hybrid quadrupole and orbitrap mass spectrometer. The mass spectrometer was connected to UHPLC instrument via an ESI interface. Samples were analyzed in negative ion mode by the full-scan-dd MS 2(data-dependent MS/MS) scanning mode. Then the constituents of

thellungiana

were identified by compared HRMS data with those of the standard compounds

MS cleavage mechanism and the related literatures.

Result:

Based on the characteristic mass data of accurate molecular weight and fragmentation ion information

29 chemical constituents were identified including 19 chlorogenic acids

7 flavonoids and 3 phenolic acid. Among them

the identified components except luteolin-7-

-glucuronopyranoside and apigenin-7-

-glucuronopyranoside were reported in

thellungiana

for the first time.

Conclusion:

The established UHPLC-Q-Orbitrap HRMS method can be used to identify the chemical constituents of

thellungiana

quickly and accurately

providing the scientific evidence for its quality evaluation and material basis research.

关键词

Keywords

references

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Rapid Identification of Chemical Constituents of Pimpinella thellungiana by UHPLC-Q-Orbitrap HRMS

DOI：10.13422/j.cnki.syfjx.20200516

摘要

Abstract

关键词

Keywords

references