Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-<italic style="font-style: italic">α</italic>

Lian-hua HE; Qing-xia QIN; Han WANG; Qing-wen WANG

doi:10.13422/j.cnki.syfjx.20200536

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Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-α

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-α
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 11-17(2020)
- 作者机构：
  
  北京大学　深圳医院，广东深圳　 518036
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200536
  CLC： R2-0; R285; R289; R364.4
- Received：26 September 2019，
  
  Published Online：19 November 2019，
  
  Published：05 March 2020
- 稿件说明：
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Lian-hua HE, Qing-xia QIN, Han WANG, et al. Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-α[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 11-17.
DOI：

Lian-hua HE, Qing-xia QIN, Han WANG, et al. Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-α[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 11-17. DOI： 10.13422/j.cnki.syfjx.20200536.

摘要

目的：

二妙散(EMS)对肿瘤坏死因子(TNF)-

诱导的人类风湿关节炎(RA)成纤维样滑膜细胞(FLS)增殖、迁移、黏附、侵袭、分泌功能的作用及其可能机制。

方法：

TNF-

(20 μg·L

-1

)体外诱导RA患者FLS，加入不同质量浓度二妙散(0.2，0.4，0.8 g·L

-1

)作用后，分别采用噻唑蓝(MTT)比色法，transwell迁移、黏附及侵袭实验检测FLS细胞的增殖活性、迁移、黏附及侵袭能力，酶联免疫吸附测定(ELISA)检测细胞上清中白细胞介素(IL)-1

的含量。提取FLS中的蛋白，采用蛋白免疫印迹法(Western blot)检测磷酸化Janus激酶1(p-JAK1)，p-信号转导与转录激活因子(STAT)1，p-STAT6的蛋白表达水平。

结果：

与空白组比较，TNF-

(20 μg·L

-1

)能增加FLS细胞的增殖、迁移、黏附、侵袭能力及IL-1

分泌(

0.01)；与TNF-

组比较，二妙散(0.2，0.4，0.8 g·L

-1

)作用24 h对TNF-

诱导的FLS细胞增殖活性没有明显影响，作用24 h内还能明显降低TNF-

诱导升高的FLS细胞迁移、黏附、侵袭能力及分泌IL-1

(

0.05)；与空白组比较，TNF-

能分别诱导p-JAK1，p-STAT1，p-STAT6在FLS中的异常升高(

0.01)，0.2，0.4，0.8 mg·L

-1

的二妙散能明显降低p-JAK1，p-STAT1，p-STAT6的表达水平(

0.05，

0.01)。

结论：

二妙散可降低FLS细胞增殖、迁移、黏附、侵袭及IL-1

的分泌，而其机制可能与JAK/STAT信号通路相关。

Abstract

Objective:

To study the effects of Ermiaosan on migration

adhesion and invasion of human fibroblast-liked synovial cells(FLS) and explore its mechanism.

Method:

Using the human FLS as the research object

the nontoxic concentration of FLS.FLS was determined by methyl thiazolyl tetrazolium (MTT) colorimetric assay for the follow-up experiment. The transwell migration

adhesion and transwell invasion test were used to detect the migration and adhesion of the different concentration of Ermiaosan on FLS

respectively. The expression of interleukin (IL)-1 beta of FLS supernatant was detected by enzyme linked immunosorbent assay (ELISA). Protein in FLS was extracted and protein expression levels of phosphorylated Janus kinase 1 (p-JAK1)

p-signal transducer and activator of transcription (STAT1) and p-STAT6 were detected by Western blot.

Result:

Compared with control group

tumor necrosis factor(TNF)-

(20 μg·L

-1

) increased the proliferation

migration

adhesion

invasion and the secretion of IL-1

of FLS (

0.01). Ermiaosan(0.2

0.4

0.8 mg·L

-1

) had no significant effect on the proliferation of FLS induced by TNF-

for 24 h. Within 24 h

the migration

adhesion

invasion

and secretion of IL-1

of FLS cells induced by TNF-

were also decreased significantly(

0.05

0.01). Compared with the blank group

TNF-

could induce abnormal elevation of p-JAK1

p-STAT1 and p-STAT6 in FLS (

0.01)

while Ermiaosan of 0.2

0.4

0.8 g·L

-1

could significantly reduce the expression levels of p-JAK1

p-STAT1 and p-STAT6 (

0.05

0.01).

Conclusion:

Ermiaosan can inhibit the migration

adhesion and invasion of FLS

and its mechanism may be related to the inhibition of the secretion of IL-1

the mechanism may be related to JAK/STAT pathway.

关键词

Keywords

references

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Related Author

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Qing-xia QIN

Qing WU

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Qing-wen WANG

Yi-qun LI

Related Institution

Shenzhen Hospital， Peking University

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⁰

Effect of Ermiaosan on Function of Human Fibroblast Synovial Cells Induced by TNF-α

DOI：10.13422/j.cnki.syfjx.20200536

摘要

Abstract

关键词

Keywords

references