Exploring Anti-inflammation Mechanism of Pien Tze Huang via Regulating of Microglia Polarization

Hai-yang SHU; Dan-cai FAN; Ning ZHAO; Cheng LYU; Ai-ping LYU; Xiao-juan HE

doi:10.13422/j.cnki.syfjx.20200541

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Exploring Anti-inflammation Mechanism of Pien Tze Huang via Regulating of Microglia Polarization

Pharmacology | 更新时间：2021-02-09

- Exploring Anti-inflammation Mechanism of Pien Tze Huang via Regulating of Microglia Polarization
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 5, Pages: 48-53(2020)
- 作者机构：
  
  1.中国中医科学院　中医临床基础医学研究所，北京　 100700
  2.广州中医药大学　第二临床医学院，广州　 510504
  3.香港浸会大学　中医药学院，香港特别行政区　 999077
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200541
  CLC： R2-0; R285; R289
- Received：29 August 2019，
  
  Published Online：20 November 2019，
  
  Published：05 March 2020
- 稿件说明：
移动端阅览
Hai-yang SHU, Dan-cai FAN, Ning ZHAO, et al. Exploring Anti-inflammation Mechanism of Pien Tze Huang via Regulating of Microglia Polarization[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 48-53.
DOI：

Hai-yang SHU, Dan-cai FAN, Ning ZHAO, et al. Exploring Anti-inflammation Mechanism of Pien Tze Huang via Regulating of Microglia Polarization[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(5): 48-53. DOI： 10.13422/j.cnki.syfjx.20200541.

摘要

目的：

从小鼠小胶质瘤细胞(BV2)极化角度探索片仔癀(PTH)的抗炎作用机制。

方法：

将对数生长期的BV2细胞分为空白组，M1模型组[脂多糖(LPS)100 μg·L

-1

＋干扰素-

(IFN-

) 10 μg·L

-1

]，M1片仔癀组[LPS 100 μg·L

-1

＋IFN-

10 μg·L

-1

＋PTH 0.4 g·kg

-1

]，M2模型组[白细胞介素-4(IL-4)20 μg·L

-1

]，M2片仔癀组[IL-4 20 μg·L

-1

＋PTH 0.4 g·kg

-1

]，给以相应处理。通过酶联免疫吸附测定(ELISA)检测细胞上清中肿瘤坏死因子-

(TNF-

)，一氧化氮(NO)，白细胞介素-10(IL-10)，转化生长因子-

(TGF-

)的含量，使用实时荧光定量聚合酶链式反应(Real-time PCR)检测细胞中一氧化氮诱导合酶(iNOS)，精氨酸-1(Arg-1)mRNA的水平，蛋白免疫印迹法(Western blot)检测细胞中iNOS，p-STAT1，p-STAT3，Arg-1，p-STAT6蛋白表达情况。

结果：

与空白组比较，M1模型组上清TNF-

，NO含量显著升高(

0.01)，细胞iNOS mRNA，iNOS，p-STAT1，p-STAT3蛋白水平显著升高(

0.01)，而与M1模型组比较，M1片仔癀组上清TNF-

及NO的含量显著下调(

0.01)，细胞iNOS mRNA显著下调(

0.01)，iNOS，p-STAT1，p-STAT3蛋白水平也明显下调(

0.05，

0.01)。与空白组比较，M2模型组细胞培养上清中IL-10及TGF-

含量显著升高(

0.01)，细胞Arg-1 mRNA，Arg-1，p-STAT6蛋白水平显著升高(

0.01)。与M2模型组比较，M2片仔癀组上清中IL-10及TGF-

含量明显上调(

0.05，

0.01)，细胞Arg-1 mRNA水平，Arg-1及p-STAT6蛋白水平明显上调(

0.05，

0.01)。

结论：

片仔癀可以通过调节BV2的极化发挥抗炎作用。

Abstract

Objective:

To investigate the anti-inflammation mechanism of Pien Tze Huang (PTH) via regulating microglia polarization.

Method:

The experiment was divided into five groups

Blank

M1[lipopolysaccharide (LPS) 100 μg·L

-1

+ interferon-

(IFN-

) 10 μg·L

-1

]

M1-PTH group[LPS 100 μg·L

-1

+ IFN-

10 μg·L

-1

+ PTH 0.4 g·kg

-1

]

M2 group[interleukin-4 (IL-4) 20 μg·L

-1

]

and M2-PTH group[IL-4 20 μg·L

-1

+ PTH 0.4 g·kg

-1

]. The concentration of nitric oxide (NO)

tumor necrosis factor-

(TNF-

)

interleukin-10 (IL-10)

and transforming growth factor-

(TGF-

) in the culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA)

the levels of inducible nitric oxide synthase(iNOS) and arginine-1 (Arg-1) mRNA were detected by real-time fluorescent quantitative polymerase chain reaction technique(Real-time PCR)

and the expression levels of p-STAT1

p-STAT3

iNOS

p-STAT6

and Arg-1 were detected by Western blot.

Result:

The concentration of NO and TNF-

of the culture supernatant

the level of iNOS mRNA

as well as the level of p-STAT1

p-STAT3 and iNOS in M1 group

which were significantly increased(

0.01) .Compared with blank group

but the concentration of NO and TNF-

were down-regulated(

0.01)

and iNOS mRNA(

0.05)

as well as the expression of iNOS

p-STAT1

and p-STAT3 was decreased (

0.05

0.01) after the invention of PTH in M1-PTH group compared with M1 group. The concentration of IL-10 and TGF-

in the culture supernatant

the mRNA level of Arg-1

as well as the levels of p-STAT6 and Arg-1 were significantly increased in M2 group when compared with Blank group

addition to the concentration of IL-10 and TGF-

were up-regulated(

0.05

0.01)

and the expression of Arg-1 mRNA

the level of Arg-1

p-STAT6 were enhanced(

0.05

0.01) in M2-PTH group compared with M2 group.

Conclusion:

PTH plays an anti-inflammatory role via regulating microglia polarization.

关键词

Keywords

references

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