Effect of Wumeiwan on Intestinal Microflora, Inflammatory Factor and Short Chain Fatty Acids in Type 2 Diabetic Rats

Guo-pei ZHOU; Fan WU; Jin-hua ZHU; Yuan GAO; Hong-jiao WAN

doi:10.13422/j.cnki.syfjx.20200605

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Effect of Wumeiwan on Intestinal Microflora, Inflammatory Factor and Short Chain Fatty Acids in Type 2 Diabetic Rats

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- Effect of Wumeiwan on Intestinal Microflora, Inflammatory Factor and Short Chain Fatty Acids in Type 2 Diabetic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 10, Pages: 8-15(2020)
- 作者机构：
  
  1.江西中医药大学　中医学院，南昌　 330004
  2.吉安市疾病预防控制中心，江西吉安　 343000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200605
  CLC： R2-0; R22; R285.5; R289
- Received：09 September 2019，
  
  Published Online：07 December 2019，
  
  Published：20 May 2020
- 稿件说明：
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Guo-pei ZHOU, Fan WU, Jin-hua ZHU, et al. Effect of Wumeiwan on Intestinal Microflora, Inflammatory Factor and Short Chain Fatty Acids in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(10): 8-15.
DOI：

Guo-pei ZHOU, Fan WU, Jin-hua ZHU, et al. Effect of Wumeiwan on Intestinal Microflora, Inflammatory Factor and Short Chain Fatty Acids in Type 2 Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(10): 8-15. DOI： 10.13422/j.cnki.syfjx.20200605.

摘要

目的：

研究乌梅丸对2型糖尿病(T2DM)模型大鼠血糖，肠道菌群，肿瘤坏死因子-

(TNF-

)，白细胞介素-10(IL-10)以及肠道菌群发酵膳食纤维产生短链脂肪酸(SCFAs)的影响作用。

方法：

以80只SD清洁大鼠作为实验对象，从中随机选取10只为正常组，其余70只以高糖高脂乳剂灌胃8周后联合链脲佐菌素(STZ，35 mg·kg

-1

)腹腔注射建立2型糖尿病大鼠模型，空腹血糖

11.10 mmol·L

-1

即为造模成功。将造模未成功的大鼠剔除，余造模成功大鼠随机分为模型组、二甲双胍组、乌梅丸高、中、低剂量组，每组10只。正常组与模型组灌胃(

)生理盐水20 mL·kg

-1

·d

-1

，二甲双胍组

二甲双胍200 mg·kg

-1

·d

-1

，乌梅丸高、中、低剂量组分别

乌梅丸20，10，5 g·kg

-1

·d

-1

。造模和用药前后定期监测大鼠血糖和体质量，用药四周后取血及大鼠粪便。16S-rDNA高通量测序技术对肠道菌群进行基因测序，酶联免疫吸附测定(ELISA)检测大鼠血清炎性因子TNF-

，IL-10的水平，气相色谱法检测粪便中乙酸、丙酸、正丁酸的含量。

结果：

与正常组比较，模型组体质量下降趋势明显(

0.01)，拟杆菌门(Bacteroidetes)，放线菌纲(Actinobacteria)，拟杆菌属(

Bacteroides

)，梭菌属(

Clostridium

)增加，厚壁菌门(Firmicutes)，

-变形菌纲(Deltaproteobacteria)，乳酸菌(

Lactobacillus

)降低，空腹血糖、血清TNF-

水平显著升高(

0.01)，IL-10水平下降(

0.01)，短链脂肪酸乙酸、丙酸、正丁酸含量降低(

0.05，

0.01)；与模型组比较，乌梅丸高、中、低剂量组及二甲双胍组体质量下降趋势变缓，Bacteroidetes，Actinobacteria，

Bacteroides

，

Clostridium

降低，Firmicutes，DeltaProteobacteria，

Lactobacillus

增加，空腹血糖、血清TNF-

水平下降(

0.01)，IL-10水平上升(

0.01)，乙酸、丙酸、正丁酸含量上升(

0.05，

0.01)。

结论：

乌梅丸可能通过调节肠道菌群，改善炎症反应，增加短链脂肪酸的含量，以致血糖降低，从而达到对2型糖尿病的防治作用。

Abstract

Objective:

To study the effects of Wumeiwan on blood glucose

intestinal microflora

tumor necrosis factor-

(TNF-

)

interleukin-10 (IL-10) and dietary fiber fermented by intestinal microflora in type 2 diabetic (T2DM) rats.

Method:

Totally 80 SD clean rats were selected as experimental subjects

and 10 of them were randomly selected as the normal group. The remaining 70 rats were given high-sugar and high-fat emulsion for 8 weeks and intraperitoneally injected with streptozotocin (STZ

35 mg·kg

-1

) to establish the rat model of T2DM. The fasting blood glucose higher than 11.10 mmol·L

-1

was considered as a successful model. The rats that were not successfully modeled were removed

and the remaining rats that were successfully modeled were randomly divided into model group

metformin group

high-dose Wumeiwan group

medium-dose Wumeiwan group and low-dose Wumeiwan group

with 10 rats in each group. Normal group and model group received (

) normal saline (20 mL·kg

-1

·d

-1

)

while metformin group (

metformin 200 mg·kg

-1

·d

-1

)

Wumeiwan high

medium and low dose groups (

Wumeiwan 20

and 5 g·kg

-1

·d

-1

) received corresponding drugs respectively. Blood glucose and body weight of rats were monitored regularly before and after administration of the drugs. Blood and feces were collected after four weeks of administration.16S-rDNA high-throughput sequencing technology was used for gene sequencing of intestinal flora. Enzyme linked immunoassay (ELISA) was used to detect the serum levels of inflammatory factors TNF-

and IL-10 in rats

and the contents of acetic acid

propionic acid and butyric acid in feces were detected by gas chromatography.

Result:

As compared with the normal group

the body weight decreased significantly (

0.01). Bacteroidetes

Actinobacteria

Bacteroides

Clostridium

increased

Firmicutes

Deltaproteobacteria

and

Lactobacillus

decreased

fasting blood glucose and serum TNF-

levels increased significantly (

0.01)

IL-10 level decreased (

0.01)

and the contents of acetic acid

propionic acid and

-butyric acid of short-chain fatty acids decreased(

0.05

0.01) in model group. As compared with the model group

the body weight decreased; Bacteroidetes

Actinobacteria

Bacteroides

and

Clostridium

decreased. Firmicutes

DeltaProteobacteria and

Lactobacillus

increased; fasting blood glucose and serum TNF-

decreased (

0.01)

and IL-10 increased (

0.01)

contents of acetic acid

propionic acid and

-butyric acid increased in Wumeiwan high-dose

medium-dose and low-dose groups and metformin group (

0.05

0.01).

Conclusion:

Wumeiwan may prevent and treat T2DM by regulating intestinal flora

improving inflammatory response

increasing SCFAs content and reducing blood glucose.

关键词

Keywords

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