Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of <italic style="font-style: italic">Lilium davidii</italic> var. <italic style="font-style: italic">unicolor</italic>

He-ping HUI; Hui JIN; Xiao-yan YANG; Xiu-zhuang LI; Ai-yi XIN; Bo QIN

doi:10.13422/j.cnki.syfjx.20200614

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Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var. unicolor

Pharmacy Fundamentals | 更新时间：2021-02-09

- Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var. unicolor
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 170-175(2020)
- 作者机构：
  
  1.中国科学院　西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室，兰州化学物理研究所，兰州　 730000
  2.中国科学院大学，北京　 100049
  3.甘肃农业职业技术学院，兰州　 730020
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200614
  CLC： R284.2; R289; R22; R2-031
- Received：28 July 2019，
  
  Published Online：04 December 2019，
  
  Published：20 April 2020
- 稿件说明：
移动端阅览
He-ping HUI, Hui JIN, Xiao-yan YANG, et al. Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var. unicolor[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 170-175.
DOI：

He-ping HUI, Hui JIN, Xiao-yan YANG, et al. Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var. unicolor[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 170-175. DOI： 10.13422/j.cnki.syfjx.20200614.

摘要

目的：

以兰州百合鳞茎中分离和纯化得到的多糖BHP-1为主要研究对象，综合运用仪器分析和化学反应等手段研究其初步的形貌特征和化学结构。

方法：

采用热重分析(TG)及扫描电镜(SEC)技术分别分析多糖BHP-1的热稳定性和形貌结构特征；通过气相色谱-质谱联用(GC-MS)技术，结合部分酸水解、高碘酸钠氧化-Smith降解以及核磁共振波谱(NMR)分析等方法对多糖BHP-1的化学结构进一步的进行解析。

结果：

BHP-1是以1，4连接的

-吡喃葡萄糖和1，4连接的

-吡喃甘露糖为基本骨架的甘露葡聚糖，在葡萄糖和甘露糖的2位和/或3位形成主要的分支，主链或支链的末端残基主要为T-

-吡喃葡萄糖，同时在其结构片段中含有少量的

-乙酰基。热重分析显示BHP-1在220 ℃开始发生降解，520 ℃基本结束，说明BHP-1热稳定性良好。形貌分析显示BHP-1表面光滑有大量凹陷，凹陷处多为片层结构错落紧密堆积而成，并交织下陷呈不规则的孔洞。

结论：

多糖BHP-1是一种热稳定性良好，表面光滑有大量的凹陷和孔洞，并含少量

-乙酰基的甘露葡聚糖，其化学结构为首次报道。

Abstract

Objective:

To study the chemical structure and morphological features of a polysaccharide BHP-1 isolated and purified from the bulbs of

Lilium davidii

var.

unicolor

Method:

Thermogravimetry (TG) and scanning electron microscopy (SEC) was used to determine the thermal characteristics and the surface morphology changes of the polysaccharide BHP-1

respectively. The chemical structure of BHP-1 was analyzed by gas chromatography-mass spectrometer (GC-MS)

partial acid hydrolysis

sodium periodate oxidation-Smith degradation and nuclear magnetic resonance (NMR).

Result:

The results showed that the backbone of the polysaccharide BHP-1 mainly contained

-(1→4)-linked

-glucopyranosyl and

-(1→4)-linked

-mannopyanosyl

and the branches were probably linked at the

-2 and/or

-3 of the mannosyl and glucosyl residues

with

-glucopyranosyl as a terminal structure. It was a natural mannoglucose containing a small amount of

-acetyl group. BHP-1 began to degrade at 220 °C

and the degradation ended at 520 ℃

indicating a good thermal stability

smooth surface and a large number of depressions. The depressions were formed by closely interlaced and sunken sheet layers with irregular holes.

Conclusion:

BHP-1 was a natural mannoglucose with a good thermal stability

smooth surface

a large number of depressions and irregular holes

and contained a small amount of

-acetyl group. Its chemical structure was reported for the first time.

关键词

Keywords

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Related Institution

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Chemical Structure and Morphological Features of A Polysaccharide BHP-1 Purified from Bulbs of Lilium davidii var. unicolor

DOI：10.13422/j.cnki.syfjx.20200614

摘要

Abstract

关键词

Keywords

references