Isolation and Identification of Chemical Constituents from <italic style="font-style: italic">Paeonia veitchii</italic>

Chao QI; Zhong-fei ZHENG; Ying LI; Xue CUI; Qing-qiang YAO

doi:10.13422/j.cnki.syfjx.20200617

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Isolation and Identification of Chemical Constituents from Paeonia veitchii

Pharmacy Fundamentals | 更新时间：2021-02-09

- Isolation and Identification of Chemical Constituents from Paeonia veitchii
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 152-157(2020)
- 作者机构：
  
  1.济南大学，山东省医学科学院　医学与生命科学学院，济南　 250200
  2.山东省医学科学院　药物研究所，国家卫生部生物技术药物重点实验室，山东省罕少见病重点实验室，济南　 250062
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200617
  CLC： R284.2; R289; R22; R2-031
- Received：20 September 2019，
  
  Published Online：16 December 2019，
  
  Published：20 March 2020
- 稿件说明：
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Chao QI, Zhong-fei ZHENG, Ying LI, et al. Isolation and Identification of Chemical Constituents from Paeonia veitchii[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 152-157.
DOI：

Chao QI, Zhong-fei ZHENG, Ying LI, et al. Isolation and Identification of Chemical Constituents from Paeonia veitchii[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 152-157. DOI： 10.13422/j.cnki.syfjx.20200617.

摘要

目的：

研究川赤芍的化学成分。

方法：

取川赤芍(30 kg)，用95%乙醇回流提取4次，提取液过滤后合并，减压浓缩得浸膏，将浸膏加水混悬后依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇进行萃取，得到相应的5个部位，石油醚部分、二氯甲烷部分、乙酸乙酯部分、正丁醇部分和水部分。各部位分别采用硅胶，Sephadex LH-20，ODS柱色谱及中压制备液相，高压制备液相等方法对其进行分离纯化，得到的化合物用红外、质谱、核磁共振谱等波谱技术进行鉴定。

结果：

从川赤芍中分离纯化得到16个化合物，分别鉴定为(1

，5

，6

)-1，8-dihydroxypin-2-en-4-one (

)，(2-hydroxyl)-phenyl-methyl-

-xylopyranoside (

)，flufuran (

)，6′-

-vanillylpaeoniflorin (

)，2，5-二羟基肉桂酸甲酯(

)，(1

，2

，5

，6

)-1，8-dihydroxypin-4-one (

)，palbinone (

)，4-

-甲基芍药苷(

)，4-

-乙基芍药苷(

)，苯甲酰氧化芍药苷(

)，苯甲酸(

)，没食子酸(

)，没食子酸甲酯(

)，没食子酸乙酯(

)，

-谷甾醇(

)，1，2，3，4，6-

-五没食子酰葡萄糖(

)。

结论：

其中，化合物

1，2

为新的天然化合物，化合物

为首次从芍药属中分离得到，化合物

4～7

为首次从该植物中分离得到。

Abstract

Objective:

To investigate the chemical constituents from

Paeonia veitchii

Method:

veitchii

samples (30 kg) were extracted with 95% ethanol for four times and then filtrated

and the combined filtrates were concentrated under vacuum to get the extracts. After suspension with water

exaction was conducted with petroleum ether

dichloromethane

ethyl acetate

-butanol and water successively to obtain five corresponding fractions. The compounds were isolated and purified by silica gel

Sephadex LH-20

ODS column chromatography and prep-HPLC

and the structures of these compounds were determined by such spectrum technologies as infrared spectroscopy (IR)

mass spectroscopy (MS)

and nuclear magnetic resonance (NMR).

Result:

Sixteen compounds were isolated and their structures were identified as follows (1

)-1

8-dihydroxypin-2-en-4-one (

)

(2-hydroxyl)-phenyl-methyl-

-xylopyranoside (

)

flufuran (

)

6′-

-vanillylpaeoniflorin (

)

methyl 2

5-dihydroxycinnamate (

)

)-1

8-dihydroxypin-4-one (

)

palbinone (

)

-methylpaeoniflorin (

)

-ethylpaeoniflorin (

)

benzoyloxypaeoniflorin (

)

benzoic acid (

)

gallic acid (

)

methyl gallate (

)

ethyl gallate (

)

-sitosterol (

)

and 1

6-penta-

-galloyl-

-glucopyranose (

Conclusion:

Compounds

were new natural compounds; compound

was isolated from genus

Paeonia

for the first time

and compounds

4-7

were isolated from this plant for the first time.

关键词

Keywords

references

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⁰

Isolation and Identification of Chemical Constituents from Paeonia veitchii

DOI：10.13422/j.cnki.syfjx.20200617

摘要

Abstract

关键词

Keywords

references