Protective Effect of Different Medicinal Parts of <italic style="font-style: italic">Nandina domestica</italic> on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity

Han SUN; Jing LIU; Zhi-gui WU; Xiao-mei FU; Cai-ying PENG; Fang WANG; Chao YANG; Shi-xin LIU

doi:10.13422/j.cnki.syfjx.20200622

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Protective Effect of Different Medicinal Parts of Nandina domestica on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity

Topic on Compatibility of Components in Nandina domestica | 更新时间：2021-02-09

- Protective Effect of Different Medicinal Parts of Nandina domestica on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 9, Pages: 136-142(2020)
- 作者机构：
  
  1.江西中医药大学　药学院，南昌　 330004
  2.范崔生全国名中医传承工作室，南昌　 330006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200622
  CLC： R22; R242; R2-031;R285.5
- Received：26 October 2019，
  
  Published Online：06 December 2019，
  
  Published：05 May 2020
- 稿件说明：
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Han SUN, Jing LIU, Zhi-gui WU, et al. Protective Effect of Different Medicinal Parts of Nandina domestica on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 136-142.
DOI：

Han SUN, Jing LIU, Zhi-gui WU, et al. Protective Effect of Different Medicinal Parts of Nandina domestica on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 136-142. DOI： 10.13422/j.cnki.syfjx.20200622.

摘要

目的：

比较南天竹的不同药用部位对抗肿瘤药三氧化二砷的减毒作用，为南天竹的深度开发应用提供科学依据。

方法：

采用大鼠慢性三氧化二砷中毒模型，将56只SD大鼠随机分为空白组；模型组(信石40 mg·kg

-1

)；2，3-二巯基丙磺酸钠组(2，3-二巯基丙磺酸钠25 mg·kg

-1

＋信石40 mg·kg

-1

)；南天竹根组(南天竹根20 g·kg

-1

＋信石40 mg·kg

-1

)；南天竹茎组(南天竹茎20 g·kg

-1

＋信石40 mg·kg

-1

)；南天竹叶组(南天竹叶20 g·kg

-1

＋信石40 mg·kg

-1

)；南天竹果实组(南天竹果实20 g·kg

-1

＋信石40 mg·kg

-1

)。连续灌胃给药10 d。末次给药后，收集24 h内尿液，手术后收集血清、肾组织及肝组织样本，测定血肌酐(serum creatinine

SCr)及尿肌酐(urine creatinine

UCr)水平，计算内生肌酐清除率(creatinine clearance

CCr)，同时检测各组大鼠肝脏、肾脏中丙二醛(malondialdehyde

MDA)，总超氧化物歧化酶(superoxide dismutase

SOD)，过氧化氢酶(catalase

CAT)水平，肾脏及肝脏苏木精-伊红染色后显微镜下观察组织病理形态学变化。

结果：

与模型组比较，南天竹根、茎、果实联合给药组大鼠体质量显著增加(

0.01)，肾脏系数显著降低(

0.01)，大鼠UCr和CCr水平显著升高(

0.01)，肾组织中MDA含量显著下降(

0.01)，肝组织中MDA水平明显降低(

0.05)，肝、肾组织中SOD和CAT活性显著升高(

0.01)，大鼠肝、肾损害病理损伤减轻；南天竹叶联合给药组除肝脏中SOD活性与模型组无明显差异外，其余指标与以上3组变化一致。

结论：

南天竹根、茎、果实对三氧化二砷氧化应激所致肝、肾毒性有显著的保护作用，南天竹叶效果稍差。

Abstract

Objective:

To compare the effect of different medicinal parts of

Nandina domestica

in reducing toxicity of anti-tumor drug arsenic trioxide

so as to provide the scientific basis for its further development and application.

Method:

Chronic arsenic trioxide poisoning model was used in this paper. Totally 56 SD rats were randomly divided into normal group

model group (arsenolite 40 mg·kg

-1

)

sodium 2

3-dimercapto-1-propanesulfonate group (sodium 2

3-dimercapto-1-propanesulfonate 25 mg·kg

-1

+ arsenolite 40 mg·kg

-1

)

Nandinae Radix group (Nandinae Radix 20 g·kg

-1

+ arsenolite 40 mg·kg

-1

)

Nandinae Caulis group (Nandinae Caulis 20 mg·kg

-1

+ arsenolite 40 mg·kg

-1

)

Nandinae Folium group (Nandinae Folium 20 g·kg

-1

+ arsenolite 40 mg·kg

-1

)

and Nandinae Fructus group (Nandinae Fructus 20 g·kg

-1

+ arsenolite 40 mg·kg

-1

). The intragastric administration lasted for 10 days. After the last administration

urine was collected within 24 hours

serum

kidney and liver tissue samples were collected after operation

and serum creatinine (SCr) and urine creatinine (UCr) levels were measured

in order to calculate endogenous creatinine clearance rate (CCr). At the same time

the levels of malondialdehyde (MDA)

superoxide dismutase (SOD) and catalase (CAT) in liver and kidney of rats in each group were detected. Some kidneys and livers were fixed with formaldehyde

and the histopathological changes were observed under microscope after hematoxylin-eosin staining.

Result:

Compared with the model group

the rats in combination group of Nandinae Radix

Nandinae Caulis

Nandinae Fructus have a heavier body mass (

0.01)

the kidney coefficient was lower (

0.01)

the levels of UCr and CCr were significantly increased (

0.01)

the content of MDA in renal tissue was decreased significantly (

0.01)

the level of MDA in liver tissue was decreased (

0.05)

whereas the activities of SOD and CAT were significantly increased (

0.01)

the pathological damage of liver and kidney was alleviated. There was no significant difference in the activity of SOD in the liver between the Nandinae Folium combination group and the model group

but the changes of the other indexes were consistent with those of the above three groups.

Conclusion:

Nandinae Radix

Nandinae Caulis

Nandinae Fructus have significant protective effects on liver and kidney toxicity induced by arsenic trioxide oxidative stress

and Nandinae Folium was the least effective among them.

关键词

Keywords

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⁰

Protective Effect of Different Medicinal Parts of Nandina domestica on Arsenic Trioxide Hepatotoxicity and Hephrotoxicity

DOI：10.13422/j.cnki.syfjx.20200622

摘要

Abstract

关键词

Keywords

references