Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats

Ya-mei FAN; Li-ying WANG; Jian WANG; Ying-rui ZHANG; Yin FU; Xian-juan YANG; Xiao-qing GUO; Dao-yin GONG

doi:10.13422/j.cnki.syfjx.20200638

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Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats

Pharmacology | 更新时间：2021-02-09

- Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 6, Pages: 64-72(2020)
- 作者机构：
  
  1.成都中医药大学　药学院，成都　 611137
  2.成都中医药大学　附属医院，成都　 610075
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200638
  CLC： R2-0; R289; R285.5
- Received：05 September 2019，
  
  Published Online：07 December 2019，
  
  Published：20 March 2020
- 稿件说明：
移动端阅览
Ya-mei FAN, Li-ying WANG, Jian WANG, et al. Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 64-72.
DOI：

Ya-mei FAN, Li-ying WANG, Jian WANG, et al. Cardioprotective Effect of Three Borneol Prevention and Administration on AMI Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(6): 64-72. DOI： 10.13422/j.cnki.syfjx.20200638.

摘要

目的：

比较3种冰片不同剂量防治给药改善急性心肌梗死(AMI)模型大鼠作用的强弱以及对氧化应激因子的影响，为阐明其抗心肌梗死量效关系及机制提供参考。

方法：

将健康成年SPF级SD雄性大鼠随机分为假手术组、模型组、溶剂模型组、硝酸甘油组、天然冰片高、中、低剂量(0.6，0.3，0.15 g·kg

-1

)组，艾片及合成冰片高、中、低剂量(0.2，0.1，0.05 g·kg

-1

)组，共13组，每组20只。按20 mL·kg

-1

灌胃，1次/d，连续预防给药3 d；假手术组与模型组给予同体积蒸馏水，溶剂模型组给予同体积5%聚山梨酯80。干预给药第3天，末次给药30 min后，施予冠状动脉左前降支结扎造模，将造模成功大鼠连续治疗给药3 d。采用BL-420N生物系统分析仪记录大鼠心电ST段波幅及血流动力学变化；大鼠体质量及心脏称重，计算心脏脏器系数；2，3，5-氯化三苯基四氮唑(TTC)染色计算心肌梗死率；苏木素-伊红(HE)染色评价心肌病理损伤程度；按试剂盒要求，检测血清中心肌酶乳酸脱氢酶(LDH)，天门冬氨酸氨基转氨酶(AST)，肌酸激酶同工酶(CK-MB)及氧化应激因子超氧化物歧化酶(SOD)，丙二醛(MDA)的水平。

结果：

与假手术组比较，模型组大鼠ST段波幅于5 min后显著抬高，左心室舒张压(LVDP)值显著升高，左室心肌收缩成分实测最大缩短速度(Vpm)值显著降低，心脏脏器系数及心肌梗死率显著升高，心肌病理组织严重损伤，血清CK-MB，AST，LDH，MDA含量显著升高(

0.05，

0.01)。与溶剂模型组比较，天然冰片及艾片中、低剂量，合成冰片高剂量均能显著抑制大鼠心电不同时间点ST段的异常抬高，天然冰片与艾片高、中剂量、低剂量及合成冰片高剂量均可显著升高左心室收缩压(LVSP)值，降低LVDP值(

0.01)，天然冰片中、低剂量，艾片高、中，合成冰片高剂量组能显著升高左室内压上升最大速度(dp/dt max)及Vpm值(

0.05，

0.01)，天然冰片及艾片中、低剂量组均能显著降低大鼠心脏脏器系数，天然冰片高、中、低剂量与艾片，合成冰片中、低剂量组均能显著改善大鼠心肌梗死率(

0.05，

0.01)，天然冰片低剂量，艾片高、中剂量及合成冰片高剂量组能显著改善其病理损伤程度(

0.01)，艾片高剂量能显著降低CK-MB含量；艾片中、低剂量显著降低AST活性，艾片中、低剂量，合成冰片高、中、低剂量明显降低LDH活性(

0.05，

0.01)，艾片高、中剂量，合成冰片高剂量组大鼠血清SOD活性显著增加(

0.05，

0.01)，天然冰片高、中、低与艾片高、中剂量组的血清MDA水平明显降低(

0.01)。

结论：

3种冰片各剂量组能不同程度发挥心肌保护作用。此实验条件下改善心肌梗死药效呈艾片>天然冰片>合成冰片趋势，天然冰片量效呈负相关、合成冰片呈正相关，艾片无明显量效关系。

Abstract

Objective:

The effects of three different doses of borneol on acute myocardial infarction (AMI) model rats and the effects on oxidative stress factors were compared to provide reference for elucidation of the dose-effect relationship and mechanism of anti-myocardial infarction.

Method:

Healthy adult male SPF SD rats were randomly divided into sham operation group

model group

solvation model group

nitroglycerin group

Borneolum high

medium and low dose(0.6

0.3

0.15 g·kg

-1

) group

l-Borneolum and Borneolum syntheticum high

medium

low dose(0.2

0.1

0.05 g·kg

-1

) group

a total of 13 groups

20 in each group. Gavage was performed at 20 mL·kg

-1

once a day for 3 days of continuous preventive administration. The sham operation group and the model group were given the same volume of distilled water

and the solvation model group was given the same volume of 5% polysorbate 80.On the third day of the pre-administration

30 minutes after the last dose

the left anterior descending coronary artery was ligated to make a model

and the successful rats were treated for 3 days. BL-420N biological system analyzer was used to record the ST-segment amplitude and hemodynamic changes. Rat body weight and cardiac weight were weighed to calculate cardiac viscera coefficients

5-triphenyl tetrazolium chloride (TTC) staining was used to calculate the myocardial infarction rate. Hematoxylin-eosin (HE) staining was used to evaluate the degree of myocardial pathological damage. According to the kit requirements

serum levels of lactate dehydrogenase (LDH)

aspartate amino-transaminase (AST)

creatine kinase isoenzyme (CK-MB) and oxidative stress factors superoxide dismutase (SOD)

malondialdehyde (MDA) were detected.

Result:

Compared with the sham operation group

the ST segment amplitude of the model group significantly increased after 5 minutes

the left ventricular diastolic blood pressure (LVDP) value increased significantly

and the measured maximum shortening velocity (Vpm) value of the left ventricular myocardial contraction component significantly decreased. The organ coefficient and myocardial infarction rate were extremely significantly increased

and the myocardial pathological tissue was severely damaged. The serum CK-MB

AST

LDH

and MDA contents were significantly increased (

0.05

0.01). Compared with the solvation model group

the Borneolum and l-Borneolum in the middle and low

and the Borneolum syntheticum high dose groups could significantly inhibited the abnormal elevation of ST segments at different time points. The Borneolum and l-Borneolum high

medium

low

and Borneolum syntheticum high dose groups significantly increased the left ventricular systolic blood pressure (LVSP) value and decrease the LVDP value (

0.01). The Borneolum medium

low

and l-Borneolum high

medium

Borneolum syntheticum high dose groups significantly increased the maximum rate of left ventricular pressure rise (dp/dt max) and Vpm value (

0.05

0.01). The Borneolum and l-Borneolum medium

low dose groups significantly reduced rat cardiac organ coefficients. The Borneolum high

medium

low and l-Borneolum

Borneolum syntheticum medium

low dose groups significantly improved myocardial infarction in rats (

0.05

0.01). The Borneolum low

l-Borneolum high

medium

and Borneolum syntheticum high groups also significantly improved the degree of pathological damage (

0.01). High dose of l-Borneolum significantly reduced CK-MB content

medium and low dose of l-Borneolum significantly reduced AST activity

medium and low dose of l-Borneolum

high

medium and low dose of Borneolum syntheticum significantly reduced LDH activity (

0.05

0.01). Serum SOD activity of rats in l-Borneolum high

medium

and Borneolum syntheticum high dose groups increased significantly (

0.05

0.01). Serum MDA levels in Borneolum high

medium

low

and l-Borneolum high

middle dose groups significantly decreased (

0.01).

Conclusion:

Three kinds of borneol in different dose groups can play different degrees of myocardial protection. Under the experimental conditions

there was a trend of l-Borneolum>Borneolum>Borneolum syntheticum in improving the efficacy of myocardial infarction

the dose-effect of Borneolum was negatively correlated

Borneolum syntheticum was positively correlated

and no significant dose-effect relationship between l-Borneolum.

关键词

Keywords

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