Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS

Min ZHANG; Qing-qing WU; Chen-hui LI; Ling-ling XU; Yuan-yuan ZHOU; Peng HUANG; He-ping HUANG; Juan YU; Dian-lei WANG

doi:10.13422/j.cnki.syfjx.20200648

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Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS

Compatibility | 更新时间：2020-08-11

- Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 13, Pages: 79-86(2020)
- 作者机构：
  
  安徽中医药大学药学院，中药复方安徽省重点实验室，合肥 230012
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200648
  CLC： R22;R914;R28;R965;O657
- Published Online：03 January 2020，
  
  Published：05 July 2020
- 稿件说明：
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张敏,吴青青,李晨辉等.基于UPLC-Q-TOF-MS/MS技术分析不同配伍比例雄黄对青黛入血成分的影响[J].中国实验方剂学杂志,2020,26(13):79-86.

ZHANG Min,WU Qing-qing,LI Chen-hui,et al.Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):79-86.
张敏,吴青青,李晨辉等.基于UPLC-Q-TOF-MS/MS技术分析不同配伍比例雄黄对青黛入血成分的影响[J].中国实验方剂学杂志,2020,26(13):79-86. DOI： 10.13422/j.cnki.syfjx.20200648.

ZHANG Min,WU Qing-qing,LI Chen-hui,et al.Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):79-86. DOI： 10.13422/j.cnki.syfjx.20200648.

摘要

目的

基于青黄散的配伍组方，探讨不同比例雄黄与青黛配伍后青黛在大鼠血清中移行成分的差异。

方法

采用UPLC-Q-TOF-MS/MS技术对青黛供试品溶液、空白血清以及3种不同配比（青黛10 g分别配伍雄黄52.5，105，210 mg，依次记为A组，B组，C组）青黄散含药血清进行检测，结合青黛供试品溶液中鉴定的化学成分，分析3组复方中青黛在大鼠体内的移行成分差异；以3组青黄散含药血清作用于人白血病细胞HL-60细胞，采用细胞计数试剂盒-8（CCK-8）法检测各含药血清对HL-60细胞活性的影响。

结果

从青黄散A组，B组，C组含药血清中分别检测到了19，22，25个移行成分，且3组含药血清均含有来自青黛供试品溶液中的5个原型成分，分别为色胺酮，靛蓝，靛玉红，2-氨基苯甲酸和

-苯基-2-萘胺；CCK-8法检测结果表明青黄散C组含药血清对HL-60细胞的抑制作用最强。

结论

固定青黛剂量后，随着雄黄剂量的增加，含药血清中移行成分增多，且对HL-60细胞的抑制作用也逐渐增强，提示雄黄可能会促进青黛中有效成分在体内的吸收，从而增强药效，进一步阐释了不同比例雄黄和青黛的配伍规律及药效物质基础。

Abstract

Objective

To investigate the effect of different dose of Realgar compatible with Indigo Naturalis on the transitional constituents of Indigo Naturalis in rat serum based on the compatibility of Qinghuangsan.

Method

Indigo Naturalis test solution

the drug-containing serum of three different proportions of Qinghuangsan (10 g of Indigo Naturalis compatible with 52.5

105

210 mg of Realgar for group A

B and C

respectively) and blank serum were detected by UPLC-Q-TOF-MS/MS

in combination with the chemical components identified in Indigo Naturalis test solution

the differences of transitional constituents of Indigo Naturalis in rat serum from the group A

B and C were analyzed. HL-60 cells (human leukemia cells) were treated with the three groups of Qinghuangsan drug-containing serum and the effect of drug-containing serum on the activity of HL-60 cells was detected by cell counting kit-8 (CCK-8) assay.

Result

A total of 19

25 of transitional constituents were detected in Qinghuangsan drug-containing serum from group A

B and C

respectively. The three groups of drug-containing serum all contained 5 prototype components from Indigo Naturalis test solution

including tryptanthrin

indigo

indirubin

2-aminobenzoic acid and

-phenyl-2-naphthylamine

respectively. The results of CCK-8 assay showed that Qinghuangsan drug-containing serum of group C had the strongest inhibitory effect on HL-60 cells.

Conclusion

After fixed Indigo Naturalis dose

with the increase of Realgar dose

the transitional constituents in rat serum increase and the inhibitory effect on HL-60 cells also gradually enhances

which indicates that Realgar may promote the absorption of active components in Indigo Naturali

in vivo

thus enhance the efficacy

further explains the compatibility law and pharmacodynamic material basis of different proportions of Realgar and Indigo Naturalis.

关键词

Keywords

references

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