Effect of Three Chinese Herbs Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Pharmacokinetics of Dapsone in Rats

Zai-xing CHENG; Zhen-zhen CAI; Li-hong LIN; Bao-yu ZHENG; Hong CHEN

doi:10.13422/j.cnki.syfjx.20200651

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Effect of Three Chinese Herbs Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Pharmacokinetics of Dapsone in Rats

Drug Metabolism | 更新时间：2021-02-09

- Effect of Three Chinese Herbs Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Pharmacokinetics of Dapsone in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 148-155(2020)
- 作者机构：
  
  1.福建中医药大学　药学院，福州　 350122
  2.海沧区嵩屿街道社区卫生服务中心，福建厦门　 361026
  3.广州白云山和记黄埔中药有限公司，广州　 510515
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200651
  CLC： R22; R969.1; R28; C37; R943.1
- Received：18 July 2019，
  
  Published Online：11 December 2019，
  
  Published：20 April 2020
- 稿件说明：
移动端阅览
Zai-xing CHENG, Zhen-zhen CAI, Li-hong LIN, et al. Effect of Three Chinese Herbs Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Pharmacokinetics of Dapsone in Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 148-155.
DOI：

Zai-xing CHENG, Zhen-zhen CAI, Li-hong LIN, et al. Effect of Three Chinese Herbs Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Pharmacokinetics of Dapsone in Rats[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 148-155. DOI： 10.13422/j.cnki.syfjx.20200651.

摘要

目的：

研究巴戟天、吴茱萸和远志3味中药采用不同比例甘草炮制后对细胞色素P450酶(CYP)中CYP3A探针氨苯砜在大鼠体内药动学的影响，进而探究中药采用甘草炮制的目的。

方法：

采用HPLC检测CYP3A体内探针氨苯砜的含量，流动相乙腈(A)-水(B)梯度洗脱(0～5 min，10%～25%A；5～20 min，25%A)，检测波长292 nm。采用PK Solution 2.0软件模拟药动学参数。

结果：

氨苯砜给药后300 min内，与空白组比较，巴戟天生品可使其在大鼠体内的消除减慢、血药浓度升高，随着炮制用甘草比例的增加氨苯砜的消除加快、药峰浓度降低；吴茱萸生品可使氨苯砜在大鼠体内的消除减慢，血药浓度升高，达峰时间延后，采用不同比例甘草炮制后均可改变氨苯砜的药峰浓度和达峰时间，进而改变氨苯砜的消除过程；远志生品可使氨苯砜在大鼠体内的消除减慢，血药浓度增加，采用不同比例甘草炮制后均可使氨苯砜的消除加快，血药浓度明显降低。

结论：

巴戟天、吴茱萸和远志3味中药生品均可减慢氨苯砜在大鼠体内的消除过程，采用不同比例甘草炮制后可使氨苯砜的消除加快，其中采用生品与甘草饮片比例为100∶6的甘草炮制后对氨苯砜的药动学过程影响均不明显。

Abstract

Objective:

To investigate the processing purpose of Morindae Officinalis Radix (MO)

Euodiae Fructus (EF) and Polygalae Radix (PR) processed by Glycyrrhizae Radix et Rhizoma (Gly).

Method:

The content of dapsone in rat plasma was determined by high performance liquid chromatography (HPLC)

the mobile phase was acetonitrile (A)-water (B) for gradient elution (0-5 min

10%-25%A; 5-20 min

25%A) and detection wavelength was set at 292 nm. PK Solution 2.0 software was used to simulate pharmacokinetic parameters.

Result:

Within 300 min after dapsone was administrated

compared with the control (CTL) group

the elimination of dapsone was slowed down and its plasma concentration was increased in the unprocessed product of MO (UMO) group. The elimination of dapsone was accelerated and its peak concentration (

max

) was decreased in the processed products of MO with Gly (GMO) groups

and they had positive correlation with proportion of Gly in GMO. Compared with the CTL group

the elimination of dapsone was slowed down

and its plasma concentration was increased and its peak time (

max

) was postponed in the unprocessed product of EF (UEF) group

while their

max

and

max

were changed in the processed products of EF with Gly (GEF) groups. Compared with the CTL group

the elimination of dapsone was slowed down and its plasma concentration was increased in the unprocessed product of PR (UPR) group

while the elimination was accelerated and its plasma concentration was decreased in the processed products of PR with Gly (GPR) groups.

Conclusion:

The elimination of dapsone is slowed down in rats administered with UMO

UEF and UPR

while its elimination is accelerated in rats administered with the processed products of these three herbs with different proportions of Gly. Among the proportions

effect of processed products of these three herbs with 100∶6 (ratio of unprocessed product-Gly) on pharmacokinetics of dapsone is not significant.

关键词

Keywords

references

陈西敬．药物代谢动力学研究进展 [M]．北京：化学工业出版社， 2008 ： 107 - 110 .

王宇光，马增春，梁乾德，等．基于药物代谢酶的中药十八反研究 [J]．世界科学技术—中药现代化， 2011 ， 13 ( 1 )： 36 - 40 .

马增春，王宇光，谭洪玲，等．中药与肝脏药物代谢酶之间的相互作用 [J]．世界华人消化杂志， 2016 ， 24 ( 7 )： 994 - 1001 .

王志琪，邹龙．甘草与其他药物相互作用的研究概况 [J]．中南药学， 2011 ， 9 ( 11 )： 857 - 860 .

陈红，张清华，陈鸣，等．中药“甘草汁”制法的研究概况 [J]．海峡药学， 2017 ， 29 ( 11 )： 24 - 26 .

L J ZHU , X S YANG , J ZHOU , et al . The exposure of highly toxic aconitine does not significantly impact the activity and expression of cytochrome P450 3A in rats determined by a novel ultra performance liquid chromatography-tandem mass spectrometric method of a specific probe buspirone [J]． Food Chem Toxicol ， 2013 ， 51 ： 396 - 403 .

J J WU , Z X CHENG , L J ZHU , et al . Coadministration of pinellia ternata can significantly reduce aconitum carmichaelii to inhibit CYP3A activity in rats [J]． Evid Based Complement Alternat Med ， 2014 ， doi： 10.1155/2014/734867 http://dx.doi.org/10.1155/2014/734867 .

邓少东，卢洪梅，林励，等．体内探针药物法研究巴戟天低聚糖对大鼠CYP3A4的影响 [J]．亚太传统医药， 2015 ， 11 ( 17 )： 5 - 8 .

怡悦．吴茱萸中的不可逆性CYP3A4抑制成分 [J]．国外医学·中医中药分册， 2005 ， 27 ( 5 )： 317 .

黄凌，邝少轶，张丽．吴茱萸碱与吴茱萸次碱经PXR、CAR核受体通路影响CYP3A4表达的研究 [J]．中国药房， 2013 ， 24 ( 35 )： 3274 - 3279 .

武佰玲，刘萍，高月，等．酸枣仁、远志和桔梗水提液对大鼠肝CYP450酶活性及mRNA表达的调控作用 [J]．中国实验方剂学杂志， 2011 ， 17 ( 18 )： 235 - 239 .

李照亮，董宪喆，王东晓，等．远志寡糖酯类化合物及远志口山酮Ⅲ对CYP450酶的活性影响研究 [J]．中国中药杂志， 2014 ， 39 ( 22 )： 4459 - 4463 .

M STIBOROVÁ , M RUPERTOVÁ , E FREI ． Cytochrome P450-and peroxidase-mediated oxidation of anticancer alkaloid ellipticine dictates its anti-tumor efficiency [J]． Biochim Biophys Acta ， 2011 ， 1814 ( 1 )： 175 - 185 .

程再兴，吴锦俊，陈红，等．乌头汤加半夏不同炮制品对大鼠CYP3A的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 20 )： 90 - 95 .

陈红，程再兴，吴锦俊，等．川乌配伍半夏对大鼠CYP3A的影响 [J]．中国实验方剂学杂志， 2017 ， 23 ( 2 )： 75 - 80 .

宋菲，杨健，王春，等． Cocktail探针药物法评价羊耳菊对大鼠CYP450酶活性的影响 [J]．中国实验方剂学杂志， 2019 ， 25 ( 2 )： 60 - 67 .

张亚蕾，郑天慧，刘伟，等．扶正化瘀方对原代大鼠肝细胞中CYP450的影响 [J]．中国实验方剂学杂志， 2018 ， 24 ( 9 )： 22 - 28 .

霍小位，王士中，孟祥波，等．不同18 α 和18 β -甘草酸配比对P-gp转运功能和大鼠CYP3A酶活性的影响 [J]．现代食品科技， 2018 ， 34 ( 9 )： 26 - 31 .

王宇光，杨明会，马增春，等 . 18 β -甘草酸和18 α -甘草酸对大鼠原代肝细胞CYP3A酶表达的影响 [J]．中国中药杂志， 2009 ， 34 ( 3 )： 307 - 311 .

缪萍，裘福荣，曾金，等．甘草诱导CYP3A促进附子代谢的减毒配伍机制 [J]．中华中医药杂志， 2014 ， 29 ( 9 )： 2813 - 2817 .

武洁，钟荣玲，王大为，等． HPLC-MS-MS法测定大鼠血浆中6种CYP450探针药物及甘草对CYP450酶活性的影响 [J]．中国实验方剂学杂志， 2014 ， 20 ( 23 )： 110 - 116 .

沈红，朱玲英，姚楠，等．甘草与附子配伍对乌头碱、新乌头碱、次乌头碱大鼠药动学的影响 [J]．中药材， 2011 ， 34 ( 6 )： 937 - 942 .

张广平，朱丽君，周娟，等．附子配伍甘草对CYP3A4体内活性的影响研究 [J]．中国中药杂志， 2012 ， 37 ( 15 )： 2206 - 2209 .

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