Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS

Han-wen HU; Yong-yan ZHAO; Tian-long YANG; Zhen-xing ZHENG; Teng PENG; Fang DENG

doi:10.13422/j.cnki.syfjx.20200715

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Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS

Pharmacy Fundamentals | 更新时间：2021-02-09

- Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 7, Pages: 148-155(2020)
- 作者机构：
  
  成都中医药大学　药学院　中药材标准化教育部重点实验室，四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地，成都　 611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200715
  CLC： R284.2; R289; R22; R2-031
- Received：24 August 2019，
  
  Published Online：19 December 2019，
  
  Published：05 April 2020
- 稿件说明：
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Han-wen HU, Yong-yan ZHAO, Tian-long YANG, et al. Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 148-155.
DOI：

Han-wen HU, Yong-yan ZHAO, Tian-long YANG, et al. Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(7): 148-155. DOI： 10.13422/j.cnki.syfjx.20200715.

摘要

目的：

应用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)技术，分析鉴定中药川佛手中的化学成分。

方法：

采用Thermo Scientific Accucore™ C

色谱柱(3 mm×100 mm，2.6 μm)，流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液梯度洗脱，流速0.3 mL·min

-1

，柱温30 ℃。高分辨质谱采用电喷雾离子源(ESI)，正离子模式扫描，扫描方式为全扫描/数据依赖二级扫描(Full MS/dd-MS

)。数据运用Compound Discoverer 3.0软件分析，根据化合物的精确相对分子质量、色谱保留时间、特征离子碎片信息，结合mzCloud网络数据库、中药成分高分辨质谱数据库(OTCML)、文献信息和相关对照品进行鉴定。

结果：

从川佛手中共鉴定了54个化学成分，包括黄酮类16个，香豆素类17个，柠檬苦素类3个，核苷及其碱基类6个，有机酸类2个，芳香醛类3个，氨基酸类1个，其他类成分6个。

结论：

该法可全面、准确地分析鉴定川佛手中的主要化学成分，研究结果为阐明川佛手的药效物质基础、优选质量控制指标提供了方法学参考和理论依据，对进一步开发利用川佛手资源具有指导意义。

Abstract

Objective:

To analyze and identify the chemical constituents of Citri Sarcodactylis Fructus by an ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap high-resolution mass spectrometry (UPLC-Q-Orbirap HRMS) method.

Method:

Compounds were separated on Thermo Scientific Accucore™ C

column (3 mm×100 mm

2.6 μm). The mobile phase was 0.1% formic acid solution and 0.1% formic acetonitrile solution. The flow rate was 0.3 mL·min

-1

and the column temperature was set at 30 ℃. HRMS was performed using an electrospray ion source (ESI)

and scanned in a positive ion mode by means of full scan/data dependent secondary scan (Full MS/dd-MS

). Compound Discoverer 3.0 software that could be linked to mzCloud network database and local high-resolution mass spectrometry database of traditional Chinese medicine components was used to analyze the data

based on accurate molecular mass

retention behaviors and characteristic ion fragmentation of the compounds

as well as literature information and relevant reference materials.

Result:

Totally 54 chemical constituents in Citri Sarcodactylis Fructus were identified

including 16 flavonoids

17 coumarins

3 limonoids

6 nucleosides and nucleobases

2 organic acids

3 aromatic aldehydes

1 amino acid and 6 other components.

Conclusion:

The established UPLC-Q-Orbitrap HRMS method can be used to effectively and rapidly identify main chemical constituents of Citri Sarcodactylis Fructus. The findings provide a methodological reference and theoretical foundation for defining the pharmacodynamic material base and optimizing quality control index of Citri Sarcodactylis Fructus

which is of guiding significance for further development and utilization of the resources.

关键词

Keywords

references

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