Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-<italic style="font-style: italic">κ</italic>B Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1

Yu-zhuo ZHANG; Qi SHANG; Geng-yang SHEN; Hui REN; Hai-qing AO; Xiao-bing JIANG; Ben-gen ZHOU

doi:10.13422/j.cnki.syfjx.20200838

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Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1

Pharmacology | 更新时间：2021-02-09

- Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 89-94(2020)
- 作者机构：
  
  1.广州中医药大学，广州　 510006
  2.广州中医药大学　第一附属医院，广州　 510405
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200838
  CLC： R2-0; R289; R285.5
- Received：14 September 2019，
  
  Published Online：07 January 2020，
  
  Published：20 April 2020
- 稿件说明：
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Yu-zhuo ZHANG, Qi SHANG, Geng-yang SHEN, et al. Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 89-94.
DOI：

Yu-zhuo ZHANG, Qi SHANG, Geng-yang SHEN, et al. Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 89-94. DOI： 10.13422/j.cnki.syfjx.20200838.

摘要

目的：

探讨龟甲水提液调控核转录因子-

B(NF-

B)炎症微环境促小鼠前成骨细胞系(MC3T3-E1)成骨分化的作用及机制。

方法：

体外培养MC3T3-E1细胞，并进行成骨诱导，龟甲水提液干预。实验分为空白组、成骨诱导组、龟甲水提液(20 mg·L

-1

)联合成骨诱导组。细胞增殖毒性检测试剂盒(CCK-8)法检测MC3T3-E1增殖能力，并确定最佳干预浓度；碱性磷酸酶(ALP)染色和茜素红(ARS)染色检测MC3T3-E1成骨分化能力；实时荧光定量PCR(Real-time PCR)检测NF-

B p65，NF-

B p105，白细胞介素-6(IL-6)，ALP和Ⅰ型胶原(COL-Ⅰ) mRNA的表达。

结果：

CCK-8结果显示，随着时间的增长，MC3T3-E1增殖虽无统计学差异，但呈上升趋势，而在20 mg·L

-1

质量浓度时增殖较其他组明显；ALP和ARS染色结果显示，成骨诱导组和龟甲水提液联合成骨诱导组染色阳性率较空白组明显升高；Real-time PCR结果显示，龟甲干预第7天，与空白组比较，龟甲水提液联合成骨诱导组NF-

B p105和IL-6 mRNA的表达明显降低(

0.01)，ALP和COL-Ⅰ mRNA的表达明显升高(

0.05)；龟甲干预第14天，与空白组比较，龟甲水提液联合成骨诱导组NF-

B p65，NF-

B p105和IL-6 mRNA的表达明显降低(

0.01)，ALP和COL-Ⅰ mRNA的表达明显升高(

0.05，

0.01)。

结论：

龟甲水提液能够促进MC3T3-E1成骨分化，其机制可能与抑制NF-

B炎性微环境有关。

Abstract

Objective:

To investigate the role and mechanism of Testudinis Carapax et Plastrum aqueous extract in promoting osteogenic differentiation of mouse preosteoblast cell line(MC3T3-E1) by regulating nuclear transcription factor-

B(NF-

B) inflammation microenvironment.

Method:

MC3T3-E1 cells were cultured

in vitro

and osteogenic induction (OI) was performed. Testudinis Carapax et Plastrum was prepared and treated the cells. Cells were devided into control group

osteogenic induction group and Testudinis Carapax et Plastrum (20 mg·L

-1

)with osteogenic induction group. The proliferation of MC3T3-E1 was detected by cell counting kit-8 (CCK-8)

and the optimum concentration of intervention was determined. MC3T3-E1 differentiation and osteogenic mineralization were assayed using alkaline phosphatase (ALP) and Alizarin red staining (ARS)

respectively. The expressions of NF-

B p65

NF-

B p105

interleukin-6(IL-6)

ALP and Collagen-Ⅰ(COL-Ⅰ) mRNA were detected by Real-time PCR.

Result:

The results of CCK-8 showed that the proliferation of MC3T3-E1 did not change statistically with time

but it showed an upward trend

while the proliferation at 20 mg·L

-1

was more obvious than other groups. The ALP and ARS showed that the positive staining rate of osteogenic induction group and Testudinis Carapax et Plastrum with osteogenic induction group were higher than control group.Real-time PCR results showed that on the 7

day in culture

the expression of NF-

B p105 and IL-6 mRNA in Testudinis Carapax et Plastrum with osteogenic induction group was significantly lower than that in control group (

0.01)

and the expression of ALP and COL-Ⅰ mRNA was significantly upregulated(

0.05)

on the 14

day

the expression of NF-

B p65

NF-

B p105 and IL-6 mRNA in Testudinis Carapax et Plastrum with osteogenic induction group was significantly lower than that in control group (

0.01). The expression of ALP and COL-Ⅰ mRNA was significantly increased (

0.05

0.01).

Conclusion:

Testudinis Carapax et Plastrum aqueous extract can promote osteogenic differentiation of MC3T3-E1 via a mechanism associated with the regulation of inhibition of NF-

B inflammatory microenvironment.

关键词

Keywords

references

M ZAIDI . Skeletal remodeling in health and disease [J]. Nat Med ， 2007 ， 13 ( 7 )： 791 - 801 .

J CHANG , Z WANG , E TANG , et al . Inhibition of osteoblastic bone formation by nuclear factor-kappab [J]. Nat Med ， 2009 ， 15 ( 6 )： 682 - 689 .

T LAWRENCE , M BEBIEN , G Y LIU , et al . Ikkalpha limits macrophage nf-kappab activation and contributes to the resolution of inflammation [J]. Nature ， 2005 ， 434 ( 7037 )： 1138 - 1143 .

K REDLICH , J S SMOLEN . Inflammatory bone loss: pathogenesis and therapeutic intervention [J]. Nat Rev Drug Dis ， 2012 ， 11 ( 3 )： 234 - 250 .

王建华，张吉祥．补肾壮骨汤治疗老年性骨质疏松症的疗效观察 [J]．实用中西医结合临床， 2019 ， 19 ( 9 )： 23 - 25 .

郑陶，许治国，许志宇，等．补肾活血方治疗膝骨关节炎临床疗效 [J]．中华中医药学刊， 2019 ， 37 ( 6 )： 1506 - 1509 .

任辉，张志达，梁德，等．龟板改善激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢的机制探讨 [J]．中华中医药杂志， 2016 ， 31 ( 5 )： 1858 - 1862 .

董重阳，师建平，陈欣，等．淫羊藿提取物对绝经后骨质疏松症大鼠Pge2及Β2肾上腺素受体的影响 [J]．中华中医药杂志， 2019 ， 34 ( 5 )： 2076 - 2079 .

李晓曦，陈宇恒，唐秀凤，等．淫羊藿、女贞子单用及配伍对绝经后骨质疏松症大鼠骨量及内分泌器官的影响 [J]．中国医药导报， 2018 ， 15 ( 35 )： 12 - 16 .

龚伟，郑洪新，杨鸫祥，等．鹿茸不同组分对去卵巢骨质疏松症大鼠骨组织的作用及其机制 [J]．中国实验方剂学杂志， 2019 ， 25 ( 20 )： 36 - 42 .

张玉卓，任辉，江晓兵，等．左归丸与右归丸对骨髓间充质干细胞成骨分化的影响 [J]．中华中医药杂志， 2018 ， 33 ( 5 )： 1997 - 2001 .

张玉卓，任辉，余翔，等．金匮肾气丸调控Fndc5、Bmp2对Bmscs成骨分化的作用 [J]．中国骨质疏松杂志， 2017 ， 23 ( 10 )： 1346 - 1352 .

余翔，任辉，尚奇，等．龟板诱导干细胞归巢预防绝经后骨质疏松症的机制探讨 [J]．中华中医药杂志， 2019 ， 34 ( 5 )： 2071 - 2075 .

Q SHANG , X YU , H REN , et al . Effect of Plastrum Testudinis on the proliferation and osteogenic differentiation of rbmscs by regulating p38 mapk-related genes [J]. Evid Based Complement Alternat Med ， 2019 ， doi： 10.1155/2019/6815620 http://dx.doi.org/10.1155/2019/6815620 .

刘辉，姚咏明．细胞内炎症信号通路交汇作用研究进展 [J]．中国病理生理杂志， 2005 ， 21 ( 8 )： 1607 - 1613 .

B Q CHEN , R K KANKALA , A Z CHEN , et al . Investigation of silk fibroin nanoparticle-decorated poly(l-lactic acid) composite scaffolds for osteoblast growth and differentiation [J]. Int J Nanomedicine ， 2017 ， 12 ： 1877 - 1890 .

K HU , W JIANG , H SUN , et al . Long Noncoding rna zbed3-as1 induces the differentiation of mesenchymal stem cells and enhances bone regeneration by repressing il-1Beta via Wnt/Beta-catenin signaling pathway [J]. J Cell Physiol ， 2019 ， 234 ( 10 )： 17863 - 17875 .

B FOUGERE , E BOULANGER , F NOURHASHEMI , et al . Chronic inflammation: accelerator of biological aging [J]. J Gerontol A Biol Sci Med Sci ， 2017 ， 72 ( 9 )： 1218 - 1225 .

T ALI , D LAM , M S BRONZE , et al . Osteoporosis in inflammatory bowel disease [J]. Am J Med ， 2009 ， 122 ( 7 )： 599 - 604 .

E ROMAS . Bone loss in inflammatory arthritis: mechanisms and therapeutic approaches with bisphosphonates [J]. Best Pract Res Clin Rheumatol ， 2005 ， 19 ( 6 )： 1065 - 1079 .

李翔． Il-6在糖皮质激素性骨质疏松小鼠椎体骨髓基质细胞成骨分化障碍中的作用机制研究 [D]．苏州：苏州大学， 2016 .

S ZHU , H HE , C GAO , et al . Ovariectomy-induced bone loss in tnfalpha and IL6 gene knockout mice is regulated by different mechanisms [J]. J Mol Endocrinol ， 2018 ， 60 ( 3 )： 185 - 198 .

M S HAYDEN , S GHOSH . Shared principles in Nf-kappaB signaling [J]. Cell ， 2008 ， 132 ( 3 )： 344 - 362 .

T WANG , S LI , D YI , et al . Chip regulates bone mass by targeting multiple traf family members in bone marrow stromal cells [J]. Bone Res ， 2018 ， 6 ： 10 .

E JIMI , K AOKI , H SAITO , et al . Selective inhibition of Nf-kappa B blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo [J]. Nat Med ， 2004 ， 10 ( 6 )： 617 - 624 .

S VAIRA , M ALHAWAGRI , I ANWISYE , et al . Rela/P65 promotes osteoclast differentiation by blocking a rankl-induced apoptotic jnk pathway in mice [J]. J Clin Invest ， 2008 ， 118 ( 6 )： 2088 - 2097 .

D V NOVACK . Role of Nf-kappab in the skeleton [J]. Cell Res ， 2011 ， 21 ( 1 )： 169 - 182 .

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Effect of Testudinis Carapax et Plastrum Aqueous Extract Regulating NF-κB Inflammation Microenvironment on Osteogenic Differentiation of MC3T3-E1

DOI：10.13422/j.cnki.syfjx.20200838

摘要

Abstract

关键词

Keywords

references