Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from <italic style="font-style: italic">Dictamnus dasycarpus</italic>

Kai ZHANG; Hong ZHAO; Yu ZHANG; Li-hong WANG; Yu SHEN; Yu-liang WANG

doi:10.13422/j.cnki.syfjx.20200914

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Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from Dictamnus dasycarpus

Pharmacy Fundamentals | 更新时间：2021-02-09

- Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from Dictamnus dasycarpus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 9, Pages: 157-166(2020)
- 作者机构：
  
  1.佳木斯大学　药学院，黑龙江佳木斯　 154007
  2.黑龙江中医药大学　药学院，哈尔滨　 150040
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200914
  CLC： R22; R289; R2-031; R284.2
- Received：08 October 2019，
  
  Published Online：16 January 2020，
  
  Published：05 May 2020
- 稿件说明：
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Kai ZHANG, Hong ZHAO, Yu ZHANG, et al. Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from Dictamnus dasycarpus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 157-166.
DOI：

Kai ZHANG, Hong ZHAO, Yu ZHANG, et al. Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from Dictamnus dasycarpus[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 157-166. DOI： 10.13422/j.cnki.syfjx.20200914.

摘要

目的：

对白鲜皮粗多糖(DDP)进行分离纯化，并研究其抗银屑病作用。

方法：

DDP经膜分离技术截留相对分子质量小于10 kDa的组分(DDP-UF)，采用DEAE-52纤维素柱分离纯化得到4种组分(DDP-UF-1，DDP-UF-2，DDP-UF-3，DDP-UF-4)；并通过红外光谱法，高效凝胶渗透色谱法(HPGPC)，高效液相色谱法(HPLC)，扫描电镜(SEM)测定其理化性质及结构特征。选用咪喹莫特乳膏诱导银屑病小鼠模型，己烯雌酚诱导雌鼠阴道上皮细胞增殖，酶联免疫吸附测定法(ELISA)检测各组小鼠血清白细胞介素(IL)-17与IL-23含量，苏木精-伊红(HE)染色法观察小鼠背部皮肤组织病理变化、阴道上皮细胞有丝分裂指数变化。

结果：

DDP-UF-1～4均具有多糖特征吸收峰，DDP-UF-1～4相对分子质量分别为10 948，40 148，32 222，19 943 Da；单糖组成及摩尔比分别为甘露糖-葡萄糖-半乳糖(32.45∶11.35∶8.69)，甘露糖-鼠李糖-葡萄糖醛酸-葡萄糖-木糖(25.68∶23.44∶21.62∶18.86∶3.68)，甘露糖-鼠李糖-葡萄糖醛酸-半乳糖醛酸-木糖-半乳糖(18.68∶4.61∶3.89∶1.65∶5.36∶6.21)，葡萄糖醛酸-半乳糖醛酸-葡萄糖-木糖-半乳糖(11.63∶15.26∶5.32∶2.08∶3.46)；扫描电镜(SEM)显示DDP-UF-1～4形态结构为片状或海绵状结构。DDP-UF-1与DDP-UF-3可改善银屑病小鼠背部皮损状态、抑制雌鼠阴道上皮细胞有丝分裂、明显降低血清IL-17，IL-23含量(

0.05，

0.01)。

结论：

DDP-UF-1与DDP-UF-3均具有良好的抗银屑病作用，其作用可能与抑制IL-23/IL-17信号通路有关。

Abstract

Objective:

To extract crude polysaccharides from

Dictamnus dasycarpus

(DDP) for separation and purification

and study its anti-psoriasis effect.

Method:

After interception of DDP with a molecular weight of less than 10 kDa (DDP-UF) using membrane separation technology

four components (DDP-UF-1

DDP-UF-2

DDP-UF-3

DDP-UF-4) were isolated and purified by DEAE-52 cellulose column. Then

the physical and chemical properties and structural characteristics of DDP-UF-1-4 samples were determined by infrared spectroscopy

high performance gel permeability chromatography (HPGPC)

high performance liquid chromatography (HPLC) and scanning electron microscope (SEM). Imiquimod cream was selected to induce mouse models of psoriasis

diethylstilbestrol was used to induce vaginal epithelial cell proliferation in female mice

interleulein-17(IL-17) and IL-23 contents of serum in each mouse group were detected by enzyme-linked immunosorbent assay (ELISA)

and skin tissues of the mouse back and vaginal epithelial cells had mitotic index changes by hematoxylin-eosin staining (HE).

Result:

DDP-UF-1-4 all exhibited the characteristic absorption peaks of polysaccharide

and the molecular weights of DDP-UF-1-4 were 10 948

40 148

32 222 and 19 943 Da

respectively. The monosaccharide compositions and mole ratios of DDP-UF-1-4 were mannose-glucose-galactose(32.45∶11.35∶8.69)

mannose-rhamnose-glucuronic acid-glucose-xylose(25.68∶23.44∶21.62∶18.86∶3.68)

mannose-rhamnose-glucuronic acid-galacturonic acid-xylose-galactose(18.68∶4.61∶3.89∶1.65∶5.36∶6.21)

glucuronic acid-galacturonic acid-glucose-xylose-galactose(11.63∶15.26∶5.32∶2.08∶3.46)

respectively. SEM showed that the morphological structures of DDP-UF-1-4 were flaky or spongy. The drug groups of DDP-UF-1 and DDP-UF-3 improved the skin condition of the psoriasis mice back

inhibited mitosis of female vaginal epithelial cells and significantly reduced the contents of IL-17 and IL-23 in serum (

0.05

0.01).

Conclusion:

Both DDP-UF-1 and DDP-UF-3 have good anti-psoriasis effects

which may be related to the inhibition of IL-23/IL-17 signaling pathway.

关键词

Keywords

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⁰

Purification, Characterization and Anti-psoriasis Effects of Polysaccharides from Dictamnus dasycarpus

DOI：10.13422/j.cnki.syfjx.20200914

摘要

Abstract

关键词

Keywords

references