Antihypertensive Effect of Tianmu Jiangya Powder on SHR Rats and Its Mechanism

Ting-ting ZHAO; Ming GONG; Zhi DAI; Xue-li LI; Juan-hui DUAN; Ruo-cong YANG; Yao-lan DAI; Shao-jing LI; Zhi-yong YAN

doi:10.13422/j.cnki.syfjx.20200936

您当前的位置：

首页 >

文章列表页 >

Antihypertensive Effect of Tianmu Jiangya Powder on SHR Rats and Its Mechanism

Pharmacology | 更新时间：2021-02-09

- Antihypertensive Effect of Tianmu Jiangya Powder on SHR Rats and Its Mechanism
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 9, Pages: 43-52(2020)
- 作者机构：
  
  1.西南交通大学　生命科学与工程学院，成都　 610031
  2.北京中医药大学，北京　 100029
  3.华润三九医药股份有限公司，广东深圳　 518110
  4.成都华西海圻医药科技有限公司，成都　 610031
  5.中国中医科学院　中药研究所，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200936
  CLC： R2-0; R289; R285.5
- Received：10 December 2019，
  
  Published Online：16 January 2020，
  
  Published：05 May 2020
- 稿件说明：
移动端阅览
Ting-ting ZHAO, Ming GONG, Zhi DAI, et al. Antihypertensive Effect of Tianmu Jiangya Powder on SHR Rats and Its Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 43-52.
DOI：

Ting-ting ZHAO, Ming GONG, Zhi DAI, et al. Antihypertensive Effect of Tianmu Jiangya Powder on SHR Rats and Its Mechanism[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(9): 43-52. DOI： 10.13422/j.cnki.syfjx.20200936.

摘要

目的：

以SHR大鼠为模型，探讨天母降压散的降压作用及其相关降压机制，为临床应用天母降压散治疗高血压病提供实验依据。

方法：

SHR雄性大鼠60只，适应性喂养1周后，按体质量随机分为6组，分别为模型组，缬沙坦组(12 mg·kg

-1

)，卡托普利组(9 mg·kg

-1

)，氢氯噻嗪组(6.25 mg·kg

-1

)，天母降压散低剂量组(0.36 g·kg

-1

)，天母降压散高剂量组(1.44 mg·kg

-1

)，以WKY大鼠作为正常组，连续灌胃给药16周。采用Softron BP-2010A智能无创血压计测量大鼠尾动脉收缩压(SBP)和心率(HR)；采用Adobe Photoshop CS5软件分别对左耳廓、后足固定选取区域对图像进行分析，以评估其对血瘀证的影响；采用Vevo 2100小动物超声影像系统检测大鼠心脏左心室射血分数(LVEF)，短轴缩短(FS)，左心室收缩末期容积(LVESV)，左心室舒张末期容积(LVEDV)，左心室收缩末期内径(LVIDs)，左心室舒张末期内径(LVIDd)，收缩末期室间隔厚度(IVSs)，舒张末期室间隔厚度(IVSd)；然后处死大鼠并取材(血液，心脏，主动脉，肝脏，肾脏，胫骨)，记录心、肝、肾的质量和胫骨长度；苏木素-伊红(HE)染色观察心脏及胸主动脉病理变化；分离血清血浆，采用硝酸还原酶法测定血清一氧化氮(NO)的含量；采用放射免疫法检测血浆3甲氧基肾上腺素(MN)，尿素(UREA)及尿酸(UA)的含量；采用免疫组化法检测分析各组胸主动脉一氧化氮合酶(iNOS)和血管内皮生长因子(VEGF)蛋白表达情况。

结果：

与正常组比较，模型组大鼠SBP及HR升高(

0.05)，8周和16周后足r值降低，g值升高(

0.05)，LVEF，FS降低，LVESV，LVIDs及IVSd升高(

0.05)，心脏质量，心脏质量/胫骨长，肝脏质量，肝脏质量/胫骨长，血浆MN，UREA及UA含量均显著升高，并促进主动脉iNOS，VEGF蛋白的表达(

0.05)。与模型组比较，天母降压散给药组可持续降低SHR大鼠SBP，维持HR稳定(

0.05)，升高后足r值，降低后足g值(

0.05)，LVEF，FS升高，LVEDV，LVESV，LVIDd，LVIDs降低(

0.05)，升高血清NO含量，降低肝脏质量，肝脏质量/胫骨长，血浆MN，UREA，UA含量(

0.05)，并下调主动脉iNOS，VEGF蛋白的表达(

0.05)。

结论：

天母降压散具有一定的降压作用，其作用机制可能主要与保护心功能，改善血管内皮功能，降低儿茶酚胺及镇静镇痛有关。

Abstract

Objective:

To investigate the antihypertensive effect of Tianmu Jiangya powder and its related antihypertensive mechanism by using SHR rats as a model

and protein expressions provide an experimental basis for the clinical application of Tianmu Jiangya powder in the treatment of hypertension.

Method:

Sixty male SHR rats were randomly divided into six groups according to body weight after one week of adaptive feeding: model group

valsartan group (12 mg·kg

-1

)

captopril group (9 mg·kg

-1

)

hydrochlorothiazide group (6 mg·kg

-1

)

Tianmu Jiangya powder low and high-dose group (0.36

1.44 g·kg

-1

)

WKY rats were used as the normal group

and the intragastric administration lasted for 16 weeks. Softron BP-2010A intelligent non-invasive blood pressure meter was used to measure the systolic blood pressure (SBP)and heart rate (HR) of rat tail arteries. Adobe Photoshop CS5 software was used to analyze the left auricle and claw fixed selected areas to evaluate the effect on blood stasis syndrome. Vevo 2100 small animal ultrasound imaging system detects left ventricular ejection fraction (LVEF)

left ventricular shortening (FS)

left ventricular end-systolic volume (LVESV)

left ventricular end-diastolic volume (LVEDV)

left ventricular end-systole dimension (LVIDs)

left ventricular end-diastole dimension (LVIDd)

interventricular septum end-systolic depth (IVSs)

and interventricular septum end-diastolic depth (IVSd). Then the rats were sacrificed and the materials were taken (blood

heart

aorta

liver

kidney

tibia)

and the weight of heart

liver

kidney and tibia length were measured and recorded. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of the heart and thoracic aorta. Separation of serum and plasma

and determination of nitric oxide (NO) in serum by nitrate reductase method. Radioimmunoassay was used to detect plasma adrenaline/3 methoxyadrenaline (MN)

urea (UREA)

and uric acid (UA) contents. The expression of nitric oxide synthase(iNOS) and vascular endothelial growth factor(VEGF) protein in thoracic aorta of each group was detected and analyzed by immunohistochemical method.

Result:

Compared with normal group

the SBP and HR of the rats in model group were significantly increased (

0.05). The r value of the claw was significantly reduced and the g value was significantly increased at 8 and 16 weeks (

0.05). LVEF and FS significantly decreased

LVESV

LVIDs

IVSd increased significantly (

0.05). Heart weight

heart weight /tibia length

liver weight and liver weight /tibia length

plasma of MN

UREA

and UA contents significantly increased

and promoted the expression of iNOS and VEGF proteins in the aortic (

0.05). Compared with the model group

the Tianmu Jiangya powder administration group could continuously reduce SBP in SHR rats

maintain HR stability (

0.05)

significantly increase the claw of r value

lower the claw of g value(

0.05). LVEF

FS significantly increased

LVEDV

LVESV

LVIDd and LVIDs significantly decreased (

0.05)

significantly increased serum NO content

decreased liver weight

liver weight/tibia length

plasma MN

UREA

UA content (

0.05)

and down-regulated the expression of iNOS and VEGF protein in the aorta(

0.05).

Conclusion:

Tianmu Jiangya powder has a certain antihypertensive effect

and its mechanism may be mainly related to protecting heart function

improving vascular endothelial function

reducing catecholamines and sedative analgesia.

关键词

Keywords

references

A V CHOBANIAN , G L BAKRIS , H R BLACK , et al . Seventh report of the joint national committee on prevention，detection, evaluation, and treatment of high blood pressure [J]． Hypertension ， 2003 ， 42 ( 11 )： 1206 - 1252 .

卢健棋，庞延，黄舒培，等．中医药治疗高血压病异常血压昼夜节律的研究近况 [J]．中华中医药学刊， 2018 ， 36 ( 2 )： 371 - 373 .

王东剑．活血利水中药复方联合西药对于高血压早期肾损害的临床疗效观察 [J]．中医临床研究， 2015 ， 7 ( 26 )： 95 - 96 .

陈厚斌，郑宇明，喻佛定．防己黄芪汤加味治疗高血压早期肾损害气虚湿阻证的临床疗效 [J]．中国实验方剂学杂志， 2019 ， 25 ( 19 )： 68 - 73 .

陈帅，韦姗姗，贾勇，等．代谢组学在高血压研究中的应用 [J]．中国实验方剂学杂志， 2020 ， 26 ( 2 )： 210 - 217 .

徐男，王亮，时海燕，等．基于整合药理学平台探究半夏白术天麻汤治疗高血压的分子机制 [J]．中国实验方剂学杂志， 2019 ， 25 ( 2 )： 109 - 117 .

陈凯，刘馨，管怡晴，等．基于网络药理学构建新型中药复方降压抗纤方抗高血压心肌纤维化的作用 [J]．中国实验方剂学杂志， 2018 ， 24 ( 20 )： 115 - 122 .

温改艳，袁洪，舒婧娴，等．抗高血压药物的研究现状 [J]．中国临床药理学杂志， 2017 ， 33 ( 24 )： 2629 - 2632 .

S CHEN . Essential hypertension: perspectives and future directions [J]． J Hypertens ， 2011 ， 30 ( 1 )： 42 - 45 .

罗琳璇，汪涛，孙兰军．天麻治疗高血压病的研究进展 [J]．中西医结合心脑血管病杂志， 2014 ， 12 ( 11 )： 1392 - 1393 .

单雅蒙，王洋，徐贵成．天麻治疗高血压病研究进展 [J]．世界中医药， 2017 ， 12 ( 12 )： 3182 - 3185 .

韩大荣．天麻研究新进展 [J]．中国处方药， 2018 ， 16 ( 4 )： 19 - 21 .

马洪田，郭雪申．珍珠降压包合物治疗高血压病的实验研究 [J]．光明中医， 2012 ， 27 ( 11 )： 2191 - 2193 .

王智先，张少强，杜武勋，等．天麻钩藤饮治疗肝阳上亢型高血压病机制探讨 [J]．中医杂志， 2013 ， 54 ( 22 )： 1911 - 1913 .

张留记，张海波，屠万倩，等．怀菊花总黄酮对自发性高血压大鼠的降压作用及机制研究 [J]．天然产物研究与开发， 2015 ， 27 ( 4 )： 592 - 597 .

陈偶英，谭元生，何诗雯，等．复方钩藤降压片对自发性高血压大鼠心脏重构的保护作用及机制探讨 [J]．中国中医急症， 2017 ， 26 ( 3 )： 377-380，424 .

陈兰玉．基于PPAR信号通路探讨桑杞清眩颗粒对高血压心肌纤维化作用机制的研究 [D]．北京：北京中医药大学， 2013 .

罗玮敏．通络干预对高血压病靶器官损伤的保护作用及机制研究 [D]．济南：山东大学， 2016 .

王勇，赵婷婷，代耀兰，等．天母降压片在小鼠模型中的镇痛、镇静作用研究 [J]．中西医结合心脑血管病杂志， 2019 ， 17 ( 3 )： 364 - 367 .

傅丽云，阮琴韵，陈雅丽，等．自发性高血压大鼠左心室心肌力学特征与心肌胶原的关系 [J]．中华高血压杂志， 2015 ， 23 ( 8 )： 736 - 742 .

H YOSHITAKA , K TAKUYA , S KOJI , et al . Imbalance of central nitric oxide and reactive oxygen species in the regulation of sympathetic activity and neural mechanisms of hypertension [J]. Am J Physiol Regul Integr Comp Physiol ， 2011 ， 300 ( 4 )： 818 - 826 .

杨晓红，李爱民，李文志．交感神经系统与高血压的研究进展 [J]．心血管病学进展， 2013 ， 34 ( 5 )： 641 - 643 .

郑易，史习宝，胡海雷．尿液儿茶酚胺水平与高血压控制水平的相关性 [J]．心脑血管病防治， 2017 ， 17 ( 6 )： 480 - 482 .

畅君毅，王素珍，闫吉霞．丹参酮ⅡA对急性心肌梗死患者心功能及hs-CRP、TNF- α 、IL-6水平的影响 [J]．中国医药科学， 2016 ， 6 ( 17 )： 142 - 145 .

刘家军，李小燕，杜成芬，等．丹参酮ⅡA磺酸钠对急性心肌梗死大鼠心肌组织间液钙离子与心功能的影响 [J]．安徽医药， 2016 ， 20 ( 4 )： 643 - 646 .

张玲，许薇，孙媛．葛根素对糖尿病大鼠心功能及心肌细胞内钙离子的影响 [J]．中国动脉硬化杂志， 2012 ， 20 ( 6 )： 532 - 535 .

周玲，邓琳，赵湜．山楂总黄酮抗心肌缺血作用及机制 [J]．中国生化药物杂志， 2011 ， 32 ( 6 )： 475 - 477 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Intervention Effect of Suanzaoren Tang on Depression Model Rats Based on JNK/c-Myc/p53 Pathway

Mechanism of Ethyl Acetate Extract of Gentianopsis paludosa in Treatment of Ulcerative Colitis Based on Bcl-2/Bax Signaling Pathway

Effect of Huatan Tongluo Prescription on Vulnerability of Carotid Atherosclerotic Plaque in Hypertension

Effect of Jianpi Yichang Powder on NLRP3/ASC/Caspase-l Signaling Pathway in Rats with Ulcerative Colitis

Effect of Guizhi Gegentang on Hemodynamics and Vascular Endothelial Function in Cervical Vertigo

Related Author

DU Shuailin

HAO Zhicheng

ZHANG Ce

GUO Jiyuan

TIAN Xusheng

LIU Na

WANG Jiaming

XU Jie

Related Institution

Heilongjiang University of Chinese Medicine

Graduate School of Heilongjiang University of Chinese Medicine

College of Pharmacy，Gansu University of Chinese Medicine

Jinan Vocational College of Nursing

Guang'anmen Hospital Jinan Hospital （Jinan Municipal Hospital of Traditional Chinese Medicine）

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰