Effect of Steaming Time and Pressure on Contents of Six Ginsenosides in Ginseng Radix et Rhizoma by UPLC-QqQ-MS/MS

Shi-wen ZHANG; Fan ZHANG; Bin-ru ZANG; Hui GAO

doi:10.13422/j.cnki.syfjx.20200946

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Effect of Steaming Time and Pressure on Contents of Six Ginsenosides in Ginseng Radix et Rhizoma by UPLC-QqQ-MS/MS

Pharmacy Fundamentals | 更新时间：2021-02-09

- Effect of Steaming Time and Pressure on Contents of Six Ginsenosides in Ginseng Radix et Rhizoma by UPLC-QqQ-MS/MS
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 8, Pages: 199-205(2020)
- 作者机构：
  
  1.辽宁中医药大学　药学院，辽宁大连　 116600
  2.广西中医药大学　药学院，广西壮瑶药工程技术研究中心，南宁　 530001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20200946
  CLC： R22; R943.1; R28; C37; O657
- Received：04 November 2019，
  
  Published Online：14 January 2020，
  
  Published：20 April 2020
- 稿件说明：
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Shi-wen ZHANG, Fan ZHANG, Bin-ru ZANG, et al. Effect of Steaming Time and Pressure on Contents of Six Ginsenosides in Ginseng Radix et Rhizoma by UPLC-QqQ-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 199-205.
DOI：

Shi-wen ZHANG, Fan ZHANG, Bin-ru ZANG, et al. Effect of Steaming Time and Pressure on Contents of Six Ginsenosides in Ginseng Radix et Rhizoma by UPLC-QqQ-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(8): 199-205. DOI： 10.13422/j.cnki.syfjx.20200946.

摘要

目的：

比较不同蒸制时长及压力对6种人参皂苷类成分总量的影响，确定红参的最佳炮制方法。

方法：

采用超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)对不同炮制工艺的红参中人参皂苷Rg

，Re，Rf，Rb

，Rc，Rb

进行含量测定，选择Waters ACQUITY UPLC BEH C

色谱柱(2.1 mm×100 mm，1.7 μm)，流动相0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B)梯度洗脱(0～4 min，81%～79%A；4～6.3 min，79%～75%A；6.3～6.5 min，75%～71%A；6.5～9.5 min，71%A；9.5～16.5 min，71%～68.5%A；16.5～16.6 min，68.5%～60%A；16.6～19 min，60%～100%A)，流速0.4 mL·min

-1

，柱温35 ℃；质谱分析采用电喷雾离子源(ESI)，负离子采集模式，毛细管电离电压2.5 kV，脱溶剂气温度350 ℃，脱溶剂气流量700 L·h

-1

，锥孔气流量50 L·h

-1

，以多反应监测(MRM)模式采集，采集范围

100～1 500，其中人参皂苷Rg

，Re，Rf，Rb

，Rc，Rb

的定量离子对分别为

799.59～637.49，945.54～475.79，799.59～475.49，1 107.59～783.97，1 077.58～783.96，1 077.75～191.19。

结果：

蒸制时长为3 h时，各样品组6种人参皂苷类成分质量分数总和处于7.099 8～16.768 5 mg·g

-1

；常压蒸制时6种人参皂苷类成分总量为加压蒸制总和的2.5～12.6倍，明显优于加压蒸制。

结论：

在本实验条件下，红参的最佳炮制方式为鲜人参常压蒸制3 h。

Abstract

Objective:

The processing method of red ginseng was determined by comparing the effects of different steaming time and pressure on the total content of six ginsenosides.

Method:

The contents of ginsenoside Rg

Rc and Rb

were determined by ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS). The Waters ACQUITY UPLC BEH C

column (2.1 mm×100 mm

1.7 μm) was used. The mobile phase was 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B) for gradient elution (0-4 min

81%-79%A; 4-6.3 min

79%-75%A; 6.3-6.5 min

75%-71%A; 6.5-9.5 min

71%A; 9.5-16.5 min

71%-68.5%A; 16.5-16.6 min

68.5%-60%A; 16.6-19 min

60%-100%A). The flow rate was set at 0.4 mL·min

-1

and the column temperature was set at 35 ℃. The mass spectrographic analysis employed electrospray ionization (ESI) and negative ion collection mode with capillary ionization voltage of 2.5 kV

desolvation temperature of 350 ℃

desolvation gas flow of 700 L·h

-1

and cone gas flow of 50 L·h

-1

. Multiple reaction monitoring (MRM) mode was used to collect information

the collection range was

100-1 500

detection was performed by MRM mode at

799.59-637.49 for ginsenoside Rg

945.54-475.79 for ginsenoside Re

799.59-475.49 for ginsenoside Rf

1 107.59-783.97 for ginsenoside Rb

1 077.58-783.96 for ginsenoside Rc

1 077.75-191.19 for ginsenoside Rb

Result:

When the steaming time was 3 hours

the total mass fraction of six ginsenosides in each sample group was 7.099 8-16.768 5 mg·g

-1

and the total amount of the six ginsenosides in atmospheric steaming was 2.5-12.6 times of that in pressurized steaming

which was obviously better than that in pressurized steaming.

Conclusion:

Under the conditions of this experiment

the best processing method of red ginseng is atmospheric steaming for 3 hours with fresh ginseng.

关键词

Keywords

references

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刘志，阮长春，刘天志，等． HPLC法同时测定林下参、鲜人参、生晒参和红参中14种人参皂苷 [J]．中草药， 2012 ， 43 ( 12 )： 2431 - 2434 .

王玉方，金春爱，赵卉，等． UPLC-ESI-MS/MS法测定人参、红参和西洋参中多种皂苷含量 [J]．特产研究， 2019 ， 41 ( 3 )： 58 - 66 .

S Y KANG , V B SCHINI-KERTH , N D KIM ． Ginsenosides of the protopanaxatriol group cause endothelium-dependent relaxation in the rat aorta [J]． Life Sci ， 1995 ， 56 ( 19 )： 1577 - 1586 .

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