Detection of Rutin in Sophorae Immaturus Flos with Electrochemical Sensor Assembled with Gold Nanoparticles/Nafion/Multi-wall Carbon Nanotube

Xiao-rong LI; Hao YAN; Hui GUO; Xu LONG; Jiang-tao LIU; Zhi LI; Dan-dan JI

doi:10.13422/j.cnki.syfjx.20201015

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Detection of Rutin in Sophorae Immaturus Flos with Electrochemical Sensor Assembled with Gold Nanoparticles/Nafion/Multi-wall Carbon Nanotube

Pharmacy Fundamentals | 更新时间：2021-02-09

- Detection of Rutin in Sophorae Immaturus Flos with Electrochemical Sensor Assembled with Gold Nanoparticles/Nafion/Multi-wall Carbon Nanotube
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 10, Pages: 156-162(2020)
- 作者机构：
  
  陕西中医药大学　药学院，咸阳　 712046
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201015
  CLC： R284.2; R289; R22; R2-031
- Received：20 September 2019，
  
  Published Online：10 February 2020，
  
  Published：20 May 2020
- 稿件说明：
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Xiao-rong LI, Hao YAN, Hui GUO, et al. Detection of Rutin in Sophorae Immaturus Flos with Electrochemical Sensor Assembled with Gold Nanoparticles/Nafion/Multi-wall Carbon Nanotube[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(10): 156-162.
DOI：

Xiao-rong LI, Hao YAN, Hui GUO, et al. Detection of Rutin in Sophorae Immaturus Flos with Electrochemical Sensor Assembled with Gold Nanoparticles/Nafion/Multi-wall Carbon Nanotube[J]. Chinese journal of experimental traditional medical formulae, 2020, 26(10): 156-162. DOI： 10.13422/j.cnki.syfjx.20201015.

摘要

目的：

采用Nafion-多壁碳纳米管(MWNTs)修饰玻碳电极，通过恒电位法在其表面电沉积纳米Au，研制一种新型的、灵敏的芦丁电化学传感器，并用于实际样品中芦丁含量的测定。

方法：

运用电化学阻抗谱(EIS)和循环伏安法(CV)研究传感器在铁氰化钾-亚铁氰化钾体系中的电化学行为，以探究传感器的电化学性能。采用CV法研究芦丁在传感器表面的电化学行为，并优化影响传感器性能的多个因素。采用差分脉冲伏安法(DPV)对芦丁的含量进行测定。

结果：

当纳米Au的沉积电位为-0.25 V，沉积时间为20 s，缓冲溶液的pH为3.0，碳纳米管的用量为6 μL时，芦丁检测的灵敏度最高。该条件下，芦丁浓度在5.0×10

-9

～7.0×10

-7

mol ·L

-1

与还原峰电流呈良好的线性关系，检出限为3.6×10

-9

mol L

-1

。

结论：

该传感器制作简单，导电性能优良，稳定性好，对芦丁的检测具有较高的灵敏高和较低的检出限。将其用于槐米样品中芦丁含量的测定，回收率介于97.6%～104.4%，为芦丁含量的测定提供了一种新的方法，为中药材的质量控制提供了一种新的思路。

Abstract

Objective:

A new-type electrochemical sensor was developed by electrodepositing gold nanoparticle on the surface of glassy carbon electrode (GCE) modified by Nafion-multi-wall carbon nanotube (MWNTs) by the potentiostatic method

and used for the detection of rutin in practical samples.

Method:

The electrochemical properties of the sensor in potassium ferricyanide-potassium ferrocyanide system were studied by electrochemical impedance spectroscopy and cyclic voltammetric method

in order to explore the electrochemical performance of the sensor. The electrochemical behavior of rutin on the surface of the sensor was studied by the cyclic voltammetric method

and the factors affecting the performance of the sensor were optimized. The content of rutin was determined by differential pulse voltammetric methods.

Result:

The optimized conditions were -0.25 V of the deposition potential of gold nanoparticle

20 s of the deposition time

pH 3.0

and 6 μL of 1 g·L

-1

carbon nanotube. Under the conditions

a good linear relationship between reduction peak current and rutin concentration was obtained from 5.0 ×10

-9

to 7.0 ×10

-7

mol·L

-1

and the detection limit was 3.6×10

-9

mol·L

-1

Conclusion:

The developed sensor has a good electrical conductivity and stability

and is highly sensitive and simple for the detection of rutin

with a low detection limit. It has been successfully applied to the detection of rutin in Sophorae Immaturus Flos with the recoveries between 97.6%and 104.4%

which provides a new method for the determination of rutin and a new idea for the quality control of Chinese medicinal materials.

关键词

Keywords

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Related Institution

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