Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction

Ru-na JIN; Li-xia HAO; Tao WANG; Hong-hua WU; Yun-tao DAI; Shou-gang SHI; Zheng-jun HUANG

doi:10.13422/j.cnki.syfjx.20201064

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Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction

Pharmacy Fundamentals | 更新时间：2020-09-09

- Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 17, Pages: 111-118(2020)
- 作者机构：
  
  1.天津中医药大学中医药研究院，天津 300193
  2.中国中医科学院中药研究所，北京 100700
  3.葵花药业集团（襄阳）隆中有限公司，湖北襄阳 441003
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201064
  CLC： R22;R28;R94;C37;O657
- Published Online：19 February 2020，
  
  Published：05 September 2020
- 稿件说明：
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靳如娜,郝丽霞,王涛等.天冬饮片与标准汤剂的质量评价方法探索[J].中国实验方剂学杂志,2020,26(17):111-118.

JIN Ru-na,HAO Li-xia,WANG Tao,et al.Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):111-118.
靳如娜,郝丽霞,王涛等.天冬饮片与标准汤剂的质量评价方法探索[J].中国实验方剂学杂志,2020,26(17):111-118. DOI： 10.13422/j.cnki.syfjx.20201064.

JIN Ru-na,HAO Li-xia,WANG Tao,et al.Analysis on Quality Evaluation Methods of Asparagi Radix Decoction Pieces and Its Standard Decoction[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):111-118. DOI： 10.13422/j.cnki.syfjx.20201064.

摘要

目的

建立天冬饮片及其标准汤剂的质量评价方法。

方法

制备10批天冬标准汤剂。采用高效液相色谱-蒸发光散射检测法（HPLC-ELSD），以乙腈-水为流动相梯度洗脱，建立天冬饮片、标准汤剂的指标成分含量测定方法以及天冬饮片的指纹图谱检测方法。采用UHPLC-LTQ-Orbitrap-MS/MS对指纹图谱中主要共有峰进行成分指认，以乙腈-0.1%甲酸水溶液为流动相，电喷雾离子源（ESI），正、负离子模式扫描，检测范围

100~1 400。

结果

天冬饮片、标准汤剂中原薯蓣皂苷与原新薯蓣皂苷的总质量分数分别为0.41%~0.72%和0.33%~0.59%，这2个成分的转移率73.6%~98.3%。天冬标准汤剂的出膏率59.0%~73.0%，pH 4.9~5.6。天冬饮片的HPLC指纹图谱中呈现了10个共有峰，且均为皂苷类成分，包括原新薯蓣皂苷，原薯蓣皂苷，天冬皂苷甲及其同分异构体，甲基原薯蓣皂苷，asparagoside F，（25

）-26-

-葡萄吡喃糖基-呋甾-5，20-二烯-3

，26-二醇-3-

-鼠李吡喃糖基（1→2）]-[

-葡萄吡喃糖基（1→4）-

-鼠李吡喃糖基（1→4）]-

-葡萄吡喃糖苷，26-

-葡萄吡喃糖基-呋甾-20（22）-烯-3

，26-二醇-3-

-鼠李吡喃糖基（1→2）]-[

-鼠李吡喃糖基（1→4）]-

-葡萄吡喃糖苷，伪原薯蓣皂苷，aspacochioside C。

结论

建立了天冬饮片及其标准汤剂的质量评价方法，且建立的方法稳定可行，可为含天冬制剂的质量评价及控制提供参考。

Abstract

Objective

To establish the quality evaluation methods of Asparagi Radix decoction pieces and its standard decoction.

Method

Ten batches of Asparagi Radix standard decoction were prepared. High performance liquid chromatography-evaporative light scattering detection method (HPLC-ELSD) was established for the determination of protodioscin and protoneodioscin in Asparagi Radix decoction pieces and its standard decoction

and the fingerprint detection of Asparagi Radix decoction pieces with acetonitrile-water as mobile phase for gradient elution. UHPLC-LTQ-Orbitrap-MS/MS was used to identify ten main common peaks in the fingerprint with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution

electrospray ionization (ESI) and positive and negative ion mode scanning were employed

the detection range was

100-1 400.

Result

The total content of protodioscin and protoneodioscin in Asparagi Radix decoction pieces was 0.41%-0.72%

and their total content in Asparagi Radix standard decoction was 0.33%-0.59%

the transfer rate of these two components was 73.6%-98.3%. The dry extract yield of the standard decoction was 59.0%-73.0%

and its pH was 4.9-5.6. There were 10 common peaks in the fingerprint

and all of them were saponins

including protoneodioscin

protodioscin

aspacochioside A and its isomer

methyl protodioscin

asparagoside F

(25

)-26-

-glucopyranosyl-furostan-5

20-diene-3

26-diol-3-

-rhamnopyranosyl (1→2)]-[

-glucopyranosyl (1→4)-

-rhamnopyranosyl (1→4)]-

-glucopyranoside

26-

-glucopyranosyl-furostan-20 (22)-ene-3

26-diol-3-

-rhamnopyranosyl (1→2)]-[

-rhamnopyranosyl (1→4)]-

-glucopyranoside

pseudodiosgenin

aspacochioside C.

Conclusion

In this paper

the quality evaluation methods of Asparagi Radix decoction pieces and its standard decoction are established

and these methods are stable and feasible

which can provide reference for the quality control of pharmaceutical preparations containing Asparagi Radix.

关键词

Keywords

references

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