Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics

Wang WEN; Li LI; De-kun LI; Lei ZHANG; Da-zheng ZHOU; Yue-wu YANG; Jian-Hui YANG; Bo-yang YU; Ai-chun JU

doi:10.13422/j.cnki.syfjx.20201073

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Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics

Pharmacy Fundamentals | 更新时间：2020-09-09

- Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 17, Pages: 104-110(2020)
- 作者机构：
  
  1.中国药科大学中药学院，南京 211198
  2.天津天士力之骄药业有限公司，天津 300410
  3.天士力控股集团有限公司，天津 300410
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201073
  CLC： R22;R28;R9;O657.6
- Published Online：25 March 2020，
  
  Published：05 September 2020
- 稿件说明：
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文旺,李莉,李德坤等.基于液质联用技术和植物代谢组学的甘草炮制品化学成分差异性分析[J].中国实验方剂学杂志,2020,26(17):104-110.

WEN Wang,LI Li,LI De-kun,et al.Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):104-110.
文旺,李莉,李德坤等.基于液质联用技术和植物代谢组学的甘草炮制品化学成分差异性分析[J].中国实验方剂学杂志,2020,26(17):104-110. DOI： 10.13422/j.cnki.syfjx.20201073.

WEN Wang,LI Li,LI De-kun,et al.Analysis of Chemical Variability on Different Processed Products of Glycyrrhiza uralensis Dry Roots and Rhizomes Based on UPLC-Q-TOF-MSE and Plant Metabolomics[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):104-110. DOI： 10.13422/j.cnki.syfjx.20201073.

摘要

目的

识别并鉴定不同甘草炮制品的质量差异标志物。

方法

运用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS

）技术，通过负离子检测模式，采集各甘草炮制品成分高精度质荷比及离子响应强度信息；利用主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）对预处理后的数据集进行分析，快速搜寻出不同甘草炮制品间的差异性成分，依据相对分子质量、碎片离子、质谱数据库和相关文献对差异性成分进行鉴定，研究甘草炮制前后成分迁移情况。

结果

从甘草片、清炒甘草、蜜炙甘草3组炮制品中共识别10个质量差异标志物，主要为甘草苷和甘草酸的衍生物，其中6''-

-acetylliquiritin apioside，6''-

-acetylliquiritin apioside异构体，6''-

-acetylliquiritin，芒柄花黄素和11-脱氧-18

-甘草亭酸在甘草片中含量最高；6''-

-acetylisoliquiritin apioside，6''-

-acetylisoliquiritin，异甘草苷和单葡萄糖醛酸甘草次酸在清炒甘草中含量最高；甘草苷在蜜炙甘草中含量最低。

结论

甘草片、清炒甘草和蜜炙甘草存在一定的化学成分差异，可为甘草炮制品的质量控制和药效研究奠定物质基础。

Abstract

Objective

To identify the quality differential markers of different processed products of

Glycyrrhiza uralensis

dry roots and rhizomes

Method

Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS

) was used to collect high-precision mass-charge ratio and ion response strength information of the components in

uralensis

dry roots and rhizomes before and after processing by negative ion mode. The data set collected after pretreatment was analyzed with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) to quickly search the differential components in different processed products of

uralensis

dry roots and rhizomes. Differential components were identified according to the relative molecular weight

fragment ion

mass spectrum database and related literature information

then the migration of components before and after processing was studied.

Result

A total of 10 quality differential markers were searched from raw products

roasted products and honey-roasted products of

uralensis

dry roots and rhizomes

mainly derivatives of liquiritin and glycyrrhizic acid. Among them

the contents of 6''-

-acetylliquiritin apioside

6''-

-acetylliquiritin apioside isomer

6''-

-acetylliquiritin

formononetin and 11-deoxo-18

-glycyrrhetic acid were the highest in the raw products

the contents of 6''-

-acetylisoliquiritin apioside

6''-

-acetylisoliquiritin

isoliquiritin and glycyrrhetic acid 3-

-glucuronide were the highest in the roasted products

the content of liquiritin was the lowest in the honey-roasted products.

Conclusion

There are some chemical differences among the three products. This study can provide material basis for the quality control and pharmacodynamic research of processed products of

uralensis

dry roots and rhizomes.

关键词

Keywords

references

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