Effect of Danggui Yinzi on Relieving Allergic Reaction in CU Model Mice by Autophagy Pathway

Feng XU; Dai-qian LI; Mei-heng ZHANG; Xiao-tong ZHANG; Qin WEI; Jing GUO; Jin-hao ZENG

doi:10.13422/j.cnki.syfjx.20201136

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Effect of Danggui Yinzi on Relieving Allergic Reaction in CU Model Mice by Autophagy Pathway

Classic Prescriptions | 更新时间：2020-08-11

- Effect of Danggui Yinzi on Relieving Allergic Reaction in CU Model Mice by Autophagy Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 12, Pages: 56-63(2020)
- 作者机构：
  
  成都中医药大学附属医院，成都 610072
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201136
  CLC： R2-0;R289;R392.8
- Published Online：04 March 2020，
  
  Published：20 June 2020
- 稿件说明：
移动端阅览
徐风,李代乾,张美恒等.基于自噬途径探讨当归饮子缓解CU模型小鼠过敏反应的效应机制[J].中国实验方剂学杂志,2020,26(12):56-63.

XU Feng,LI Dai-qian,ZHANG Mei-heng,et al.Effect of Danggui Yinzi on Relieving Allergic Reaction in CU Model Mice by Autophagy Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):56-63.
徐风,李代乾,张美恒等.基于自噬途径探讨当归饮子缓解CU模型小鼠过敏反应的效应机制[J].中国实验方剂学杂志,2020,26(12):56-63. DOI： 10.13422/j.cnki.syfjx.20201136.

XU Feng,LI Dai-qian,ZHANG Mei-heng,et al.Effect of Danggui Yinzi on Relieving Allergic Reaction in CU Model Mice by Autophagy Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):56-63. DOI： 10.13422/j.cnki.syfjx.20201136.

摘要

目的

探讨当归饮子对慢性荨麻疹（CU）小鼠模型过敏反应的影响及自噬干预机制。

方法

选用SPF级BALB/c小鼠，腹腔注射卵白蛋白与氢氧化铝悬液复制CU小鼠模型，设立分组并灌胃给药：空白组(生理盐水20 mL·kg

-1

·d

-1

)，模型组(生理盐水20 mL·kg

-1

·d

-1

)，开瑞坦组（0.001 3 g·kg

-1

·d

-1

），当归饮子高、中、低剂量组（39.3，19.6，9.8 g·kg

-1

·d

-1

）。苏木素-伊红（HE）染色观察CU小鼠皮肤组织病理学改变；透射电镜观察皮肤组织细胞的自噬形态学；免疫组化(IHC)检测皮肤组织微管相关蛋白1轻链3B（LC3B）和p62蛋白的表达；实时荧光定量聚合酶链式反应（Real-time PCR）检测皮肤组织LC3B，p62 mRNA转录水平。

结果

当归饮子可显著改善CU小鼠皮肤真皮水肿、胶原束分离、毛细血管扩张等病理表现；并促进自噬小体形成，改善胞核染色质浓聚、线粒体肿胀、内质网扩张等异常细胞超微结构；与空白组比较，模型组CU小鼠皮肤组织中LC3B蛋白表达显著增加（

0.01），LC3B mRNA水平有升高趋势，p62 mRNA水平及蛋白表达均显著降低（

0.01）；与模型组比较，当归饮子各剂量组小鼠皮肤组织LC3B mRNA水平及蛋白表达明显降低（

0.05，

0.01），小鼠皮肤组织的p62 mRNA水平及蛋白表达明显下降（

0.05，

0.01），以高剂量组疗效最佳。

结论

当归饮子可调节LC3B，p62 mRNA及蛋白表达，增强细胞自噬水平，进而改善CU小鼠皮肤病理状态。

Abstract

Objective

To investigate the effect of Danggui Yinzi on allergic reaction in chronic urticaria (CU) mice model and the mechanism of autophagy intervention.

Method

The SPF BALB/c mice were used to replicate the CU mice model by intraperitoneal injection of ovalbumin and aluminum hydroxide suspension. The animals were randomly allocated into six groups: a normal group (normal saline 20 mL·kg

-1

·d

-1

)

a model group (normal saline 20 mL·kg

-1

·d

-1

)

a loratadine group(0.001 3 g·kg

-1

·d

-1

)

a Danggui Yinzi high，medium and low-dose group（39.3，19.6，9.8 g·kg

-1

·d

-1

）. The pathological changes of skin tissues were observed by hematoxylin-eosin (HE) staining. Morphological changes of autophagy in skin tissues epithelial cells were observed by transmission electron microscope. The mRNA levels of microtubule-associated protein 1 light chain 3B(LC3B) and ubiquitin-binding protein p62 mRNA in skin tissues were detected by real-time quantitative polymerase chain reaction (Real-time PCR). The expressions of LC3B and p62 in skin tissues were detected by immunohistochemistry (IHC).

Result

Danggui Yinzi can significantly improve the pathological manifestations of dermal edema

collagen bundles separation

telangiectasia in CU mice， it can also improve autophagosomes formation and abnormal cell ultrastructure such as nuclear chromatin condensation

mitochondrial swelling

endoplasmic reticulum expansion

etc. Compared with the normal group

the protein expressions of LC3B in skin tissues of the model group was significantly increased (

0.01)

LC3B mRNA level was increased too

while p62 mRNA levels and its protein expressions were decreased-regulated (

0.01). Compared with the model group

levels of LC3B mRNA and protein expressions of the Danggui Yinzi groups were significantly increased (

0.05

0.01)

while p62 mRNA levels and its protein expressions were significantly decreased-regulated (

0.05

0.01).

Conclusion

Danggui Yinzi can regulate the expression of LC3B

p62 mRNA and protein expressions

enhance the level of autophagy

and improve the pathological state of CU mice.

关键词

Keywords

references

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