Identification of Chemical Constituents in Caulis of <italic style="font-style: italic">Erycibe schmidtii </italic>by UPLC-Q-Exactive Focus-MS/MS

Jing HU; Yuan-yuan YANG; Hui REN; Xiao-min CUI; Xiao-mei LIU; Jia-yi LUO; Zhi-yong CHEN

doi:10.13422/j.cnki.syfjx.20201162

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Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS

Pharmacy Fundamentals | 更新时间：2020-09-09

- Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 18, Pages: 124-132(2020)
- 作者机构：
  
  1.陕西省中医药研究院，西安 710061
  2.西安市食品药品检验所，西安 710054
  3.西安培华学院医学院，西安 710125
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201162
  CLC： R22;R28;R931;O657
- Received：16 January 2020，
  
  Published Online：26 March 2020，
  
  Published：20 September 2020
- 稿件说明：
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胡静,杨媛媛,任慧等.光叶丁公藤中化学成分的UPLC-Q-Exactive Focus-MS/MS鉴定[J].中国实验方剂学杂志,2020,26(18):124-132.

HU Jing,YANG Yuan-yuan,REN Hui,et al.Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):124-132.
胡静,杨媛媛,任慧等.光叶丁公藤中化学成分的UPLC-Q-Exactive Focus-MS/MS鉴定[J].中国实验方剂学杂志,2020,26(18):124-132. DOI： 10.13422/j.cnki.syfjx.20201162.

HU Jing,YANG Yuan-yuan,REN Hui,et al.Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):124-132. DOI： 10.13422/j.cnki.syfjx.20201162.

摘要

目的

利用UPLC-Q-Exactive Focus-MS/MS技术对光叶丁公藤中的化学成分进行快速识别和鉴定。

方法

选用80%甲醇提取光叶丁公藤作为供试品溶液，对光叶丁公藤药材的化学成分进行分析和鉴定。采用Thermo Accucore aQ C

色谱柱（2.1 mm×150 mm，2.6 μm）进行色谱分离，以甲醇（A）-0.1%甲酸水溶液（B）为流动相进行梯度洗脱（0~12 min，5%~25%A；12~20 min，25%~30%A；20~28 min，30%~38%A；28~40 min，38%~42%A）；质谱分析采用正、负离子监测模式和加热电喷雾离子源（HESI），扫描范围

80~1 200。

结果

共鉴定出了42个化学成分，包括香豆素类12个、绿原酸类14个、生物碱类1个、酰胺类1个，其余14个为酯化糖苷类。

结论

UPLC-Q-Exactive Focus-MS/MS技术可以快速、全面地鉴定光叶丁公藤药材中的化学成分，其中11个成分经与对照品比对后准确鉴定，31个成分在该植物中属首次发现，2个成分在丁公藤属植物中属首次发现，可为光叶丁公藤的物质基础研究和替代品开发提供重要依据。

Abstract

Objective

To rapidly recognize and identify the chemical constituents in caulis of

Erycibe schmidtii

by ultra-high performance liquid chromatography coupled with Q-Exactive Focus mass spectrometry (UPLC-Q-Exactive Focus-MS/MS).

Method

Taking 80% methanol extract of

E. schmidtii

caulis as the test solution

the chemical constituents in caulis of

E. schmidtii

were analyzed and identified. Thermo Accucure aQ C

column (2.1 mm×150 mm

2.6 μm) was used for chromatographic separation with the mobile phase of methanol (A)-0.1% formic acid solution (B) for gradient elution (0-12 min

5%-25%A; 12-20 min

25%-30%A; 20-28 min

30%-38%A; 28-40 min

38%-42%A). Positive and negative ion monitoring modes and heated electrospray ion source (HESI) were used for mass spectrographic analysis. The scanning range was

80-1 200.

Result

A total of 42 chemical constituents from caulis of

E. schmidtii

were identified

including 12 coumarins

14 chlorogenic acids

1 tropane alkaloid

1 amide and 14 esterified glycosides.

Conclusion

Chemical constituents in caulis of

E. schmidtii

can be quickly and fully identified by UPLC-Q-Exactive Focus-MS/MS. Among them

11 compounds are unambiguously identified by comparing with reference standards

31 compounds are reported for the first time in this herb

2 compounds are reported for the first time in

Erycibe

plants. This paper can provide the important basis for study on pharmacodynamic material base and substitute development of

E. schmidtii

caulis.

关键词

Keywords

references

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Identification of Chemical Constituents in Caulis of Erycibe schmidtii by UPLC-Q-Exactive Focus-MS/MS

DOI：10.13422/j.cnki.syfjx.20201162

摘要

Abstract

关键词

Keywords

references