Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by<italic style="font-style: italic"> β</italic><sub>2</sub>AR

Ming-yue ZHANG; Feng TAN; Su-min YE; Yi CHAI; Fei-xiang LIU; Cong-ying MAI; Xing LI; Dao-ming XU; Qiao-ling FAN

doi:10.13422/j.cnki.syfjx.20201224

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Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β₂AR

Classic Prescriptions | 更新时间：2020-08-11

- Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β₂AR
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 12, Pages: 49-55(2020)
- 作者机构：
  
  1.南京中医药大学基础医学院，南京 210023
  2.南京中医药大学第一附属医院，南京 210029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201224
  CLC： R22;R242;R2-031;R285.5
- Published Online：03 April 2020，
  
  Published：20 June 2020
- 稿件说明：
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张明玥,谭峰,叶素敏等.左归丸拆方通过调节β₂-AR介导的OPG/RANKL信号通路提高去卵巢大鼠骨密度[J].中国实验方剂学杂志,2020,26(12):49-55.

ZHANG Ming-yue,TAN Feng,YE Su-min,et al.Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β₂AR[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):49-55.
张明玥,谭峰,叶素敏等.左归丸拆方通过调节β₂-AR介导的OPG/RANKL信号通路提高去卵巢大鼠骨密度[J].中国实验方剂学杂志,2020,26(12):49-55. DOI： 10.13422/j.cnki.syfjx.20201224.

ZHANG Ming-yue,TAN Feng,YE Su-min,et al.Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β₂AR[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(12):49-55. DOI： 10.13422/j.cnki.syfjx.20201224.

摘要

目的

探讨左归丸及其拆方治疗绝经后骨质疏松症（PMOP）的作用机制。

方法

去卵巢法建立骨质疏松症模型，将40只SD大鼠平均随机分为假手术组，模型组（0.1 mL·kg

-1

·d

-1

），17

-雌二醇组(50 μg·kg

-1

·d

-1

）和左归丸补阳药低、高剂量（0.55，1.1 g·kg

-1

·d

-1

）组。灌胃给药12周后，显微CT（

-CT）检测右侧远端股骨骨密度（BMD）及显微结构；苏木精-伊红（HE）染色观察大鼠股骨形态学变化；酶联免疫吸附法（ELISA）检测大鼠血清中Ⅰ型胶原交联羧基端肽（

-CTX）和骨特异性碱性磷酸酶（BALP）浓度；实时荧光定量聚合酶链式反应（Real-time PCR）和蛋白免疫印迹法（Western blot）检测大鼠下丘脑

-肾上腺素能受体（

AR），胫骨平台骨保护素（OPG）和核转录因子

-κ

B受体激活因子配体（RANKL）蛋白及mRNA表达水平。

结果

与假手术组相比，模型组大鼠BMD显著下降（

0.01），骨组织形态破坏；血清BALP含量降低，

β-

CTX含量升高（

0.01）；下丘脑

AR，胫骨RANKL mRNA及蛋白表达量升高（

0.05，

0.01），胫骨OPG mRNA及蛋白表达量降低（

0.05，

0.01）。治疗后，与模型组比较，各用药组大鼠BMD升高（

0.01），骨组织形态改善；血清BALP含量升高，

-CTX含量降低（

0.01）；下丘脑

AR，胫骨RANKL mRNA及蛋白表达降低（

0.05，

0.01），胫骨平台OPG mRNA和蛋白表达升高（

0.05，

0.01）。

结论

左归丸拆方中补阳药能通过调节

AR介导的OPG/RANKL信号通路提高去卵巢大鼠骨密度，“阳中求阴”配伍特点是左归丸治疗PMOP的关键之一。

Abstract

Objective

To investigate the mechanism of decomposed Zuoguiwan（ZGW） recipes in treating ovariectomized osteoporosis rats.

Method

Forty Sprague-Dawley female rats were equally and randomly divided into Sham-operated group

ovariectomized model group

positive group

and low and high-dose ZGW groups. After 12 weeks of administration by gavage

the bone mineral density (BMD) of rats' distal femur was measured by micro-CT

the morphology of bone tissue were observed by hematoxylin-eosin staining (HE)

-cross-linked c-telopeptide of type Ι collagen (

-CTX) and bone-specific alkaline phosphatase (BALP) in serum were detected by enzyme-linked immunosorbent assay (ELISA)

and the mRNA and protein expressions of

OPG and RANKL were evaluated by Western blot analysis and real-time quantitative polymerase chain reaction （PCR）.

Result

Compared with Sham-operated group

BMD of rats in ovariectomized model group was decreased (

0.01)

morphology of bone tissue was destroyed

serum BALP was reduced

while

-CTX was boosted (

0.01)，mRNA and protein expressions of OPG in tibia were reduced

while RANKL were increased

and mRNA and protein expressions of

AR in the hypothalamus were decreased (

0.05

0.01). Compared with ovariectomized model group

BMDs of rats in low and high-dose ZGW groups were increased (

0.01)

morphology of bone tissue was repaired

serum BALP and mRNA and protein expressions of OPG in tibia were up-regulated (

0.05

0.01)

whereas serum

-CTX and mRNA and protein expressions of

AR in the hypothalamus and RANKL in tibia were down-regulated (

0.05

0.01).

Conclusion

Yang-nourishing components in decomposed Zuoguiwan recipes can improve BMD of ovariectomized rats by regulating OPG/RANKL pathway mediated by

AR. "Seeking Yin in Yang" is a crucial mechanism of Zuoguiwan in treating ovariectomized osteoporosis in rats.

关键词

Keywords

references

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Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β2AR

DOI：10.13422/j.cnki.syfjx.20201224

摘要

Abstract

关键词

Keywords

references

Effect of Decomposed Zuoguiwan Recipes in Improving BMD of Ovariectomized Rats by Regulating OPG/RANKL Pathway Mediated by β₂AR