Effect of Danggui Buxuetang on PKD1/NF-κB/MnSOD Signal Pathway in Bleomycin-induced Pulmonary Fibrosis in Rats

Na LIU; Jie-peng WANG; Chen-xi LU; Wei LI; Shuo WANG; Qian FANG; Fang FANG; Chao-yi FANG

doi:10.13422/j.cnki.syfjx.20201303

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Effect of Danggui Buxuetang on PKD1/NF-κB/MnSOD Signal Pathway in Bleomycin-induced Pulmonary Fibrosis in Rats

Classic Prescriptions | 更新时间：2020-08-11

- Effect of Danggui Buxuetang on PKD1/NF-κB/MnSOD Signal Pathway in Bleomycin-induced Pulmonary Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 13, Pages: 66-72(2020)
- 作者机构：
  
  1. 河北中医学院，石家庄 050200
  2. 河北省心脑血管病中医药防治研究重点实验室，石家庄 050091
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201303
  CLC： R2-0;R22;R285.5;R289
- Published Online：02 April 2020，
  
  Published：05 July 2020
- 稿件说明：
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刘娜,王杰鹏,鲁辰希等.当归补血汤对博莱霉素致肺纤维化大鼠PKD1/NF-κB/MnSOD信号通路的影响[J].中国实验方剂学杂志,2020,26(13):66-72.

LIU Na,WANG Jie-peng,LU Chen-xi,et al.Effect of Danggui Buxuetang on PKD1/NF-κB/MnSOD Signal Pathway inBleomycin-induced Pulmonary Fibrosis in Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):66-72.
刘娜,王杰鹏,鲁辰希等.当归补血汤对博莱霉素致肺纤维化大鼠PKD1/NF-κB/MnSOD信号通路的影响[J].中国实验方剂学杂志,2020,26(13):66-72. DOI： 10.13422/j.cnki.syfjx.20201303.

LIU Na,WANG Jie-peng,LU Chen-xi,et al.Effect of Danggui Buxuetang on PKD1/NF-κB/MnSOD Signal Pathway inBleomycin-induced Pulmonary Fibrosis in Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):66-72. DOI： 10.13422/j.cnki.syfjx.20201303.

摘要

目的

观察当归补血汤对博莱霉素致肺纤维化模型大鼠肺组织病理及蛋白激酶D1（PKD1）/核转录因子-

B（NF-

B）/锰超氧化物歧化酶（MnSOD）介导的氧化应激通路的影响，探讨该方干预肺纤维化作用机制。

方法

32只雄性SPF级SD大鼠，随机分为假手术组、模型组、当归补血汤组、泼尼松组，每组8只。除假手术组外，其余各组气管内滴注博莱霉素制备肺纤维化大鼠模型。造模24 h后，当归补血汤组给予当归补血汤水溶液（0.81 g·kg

-1

）灌胃，泼尼松组给予泼尼松水溶液（0.005 g·kg

-1

）灌胃，假手术组、模型组予等体积生理盐水灌胃。于用药14 d后，股动脉采血并分离血清，剖胸取肺。苏木素-伊红（HE）染色和马松（Masson）染色观察大鼠肺组织病理改变，并行Szapiel评分和Ashcroft评分；测定血清丙二醛（MDA）含量和超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GSH-Px）活性；采用实时荧光定量聚合酶链式反应（Real-time PCR）和蛋白免疫印迹法（Western blot）分别检测PKD1，NF-

B，MnSOD mRNA及蛋白的表达。

结果

与假手术组比较，模型组Szapiel评分和Ashcroft评分明显升高（

0.05），血清MDA含量显著升高，SOD，CAT，GSH-Px活性显著降低，肺组织PKD1，NF-

B，MnSOD mRNA及蛋白表达显著升高（

0.01）。与模型组比较，当归补血汤组明显下降Szapiel评分和Ashcroft评分（

0.05）

显著降低血清MDA含量，明显升高SOD，CAT，GSH-Px活性

明显降低肺组织PKD1，NF-

B，MnSOD mRNA及蛋白表达（

0.05，

0.01）。

结论

当归补血汤可通过调节PKD1/NF-

B/MnSOD线粒体核抗氧化通路，提高机体抗氧化能力，从而减轻肺纤维化程度。

Abstract

Objective

To observe the effect of Danggui Buxuetang on lung histopathology and protein kinase D1 (PKD1)

nuclear transcription factor-

B (NF-

B) and manganese superoxide dismutase (MnSOD)-mediated oxidative stress pathway in rats with pulmonary fibrosis induced by bleomycin

so as to explore the mechanism of intervention of pulmonary fibrosis.

Method

Thirty-two male SPF SD rats were randomly divided into sham operation group

model group

Danggui Buxuetang group and prednisone group

with 8 rats in each group. Except the sham operation group

the other groups were prepared through the intratracheal instillation with bleomycin. After modeling for 24 h

the rats of Danggui Buxuetang group were administered with Danggui Buxuetang (0.81 g·kg

-1

). The rats of prednisone group were given aqueous solution of prednisone (0.005 g·kg

-1

). The rats of sham operation group and model group were given the same volume of saline. After 14

days of administration

blood was collected from the femoral artery

serum was separated

and the lungs were taken by thoracotomy. The pathological changes of rat lung tissues were observed by hematoxylin-eosin staining (HE) and Masson trichrome staining

and graded by Szapiel score and Ashcroft score at the same time. The content of serum malondialdehyde (MDA)

and the activities of superoxide dismutase (SOD)

catalase (CAT)

glutathione peroxidase (GSH-Px) were determined. Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to measure mRNA and protein expressions of PKD1

NF-

MnSOD.

Result

Compared with the rats in sham operation group

the rats in model group had higher Szapiel scores and Ashcroft scores (

0.05)

higher serum MDA content

but lower SOD

CAT and GSH-Px activities(

0.01)

moreover

the rat lung tissues in model group had higher mRNA and protein expressions of PKD1

NF-

B and MnSOD (

0.01) than those in sham operation group. Compared with the rats in model group

the Szapiel scores and Ashcroft scores of the rats in Danggui Buxuetang group were decreased significantly（

0.05）. The serum MDA content was decreased significantly

and SOD

CAT

GSH-Px activities were increased

whereas mRNA and protein expressions of PKD1

NF-

MnSOD in the rat lung tissues were decreased（

0.05，

0.01）.

Conclusion

Danggui Buxuetang can reduce the degree of pulmonary fibrosis by regulating the anti-oxidation pathway of PKD1/NF-

B/MnSOD mitochondrial nucleus and improving the body's antioxidant capacity.

关键词

Keywords

references

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