Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3<italic style="font-style: italic">β</italic> in Astrocytes Under Glutamate Environment

Zhen-zhen TIAN; Li-juan ZHU; Hong-jiao WAN; Bu-gao ZHOU; Yi-yong XU

doi:10.13422/j.cnki.syfjx.20201304

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Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment

Classic Prescriptions | 更新时间：2020-08-11

- Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 13, Pages: 45-51(2020)
- 作者机构：
  
  1.江西中医药大学，南昌 330004
  2.江西省中西医结合医院，南昌 330003
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201304
  CLC： R2-0;R22;R285.5;R289
- Published Online：02 April 2020，
  
  Published：05 July 2020
- 稿件说明：
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田真真,朱丽娟,万红娇等.温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响[J].中国实验方剂学杂志,2020,26(13):45-51.

TIAN Zhen-zhen,ZHU Li-juan,WAN Hong-jiao,et al.Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):45-51.
田真真,朱丽娟,万红娇等.温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及PI3K，Akt，GSK3β表达的影响[J].中国实验方剂学杂志,2020,26(13):45-51. DOI： 10.13422/j.cnki.syfjx.20201304.

TIAN Zhen-zhen,ZHU Li-juan,WAN Hong-jiao,et al.Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):45-51. DOI： 10.13422/j.cnki.syfjx.20201304.

摘要

目的

探讨温胆汤含药血清对谷氨酸环境下星型胶质细胞的保护作用及磷脂酰肌醇-3激酶（PI3K）/蛋白激酶B（Akt）/糖原合成酶激酶3

（GSK3

）信号通路的影响。

方法

将60只大鼠随机分为5组

其中正常组20只，氯氮平组、温胆汤高、中、低剂量组，每组10只。正常组给予20 mL·kg

-1

生理盐水灌胃，氯氮平组给予氯氮平原药20 mg·kg

-1

灌胃，温胆汤组分别给予剂量为40，20，10 g·kg

-1

的温胆汤灌胃，1次/d，8 d后处死大鼠，取血，离心取血清，灭活，过滤除菌，离心管分装备用。将星型胶质细胞分为空白组、模型组、氯氮平组、温胆汤高、中、低剂量组，其中空白组、模型组给予空白血清培养，其余各组给予相应含药血清培养；除空白组外，其余各组用10 mmol·L

-1

谷氨酸处理24 h后予流式细胞仪检测星形胶质细胞凋亡率；蛋白免疫印迹法（Western blot），实时荧光定量聚合酶链式反应（Real-time PCR）分别检测星型胶质细胞PI3K，Akt，GSK3

蛋白和mRNA的表达。

结果

与空白组比较，模型组细胞凋亡率显著升高（

0.01）；与模型组比较，温胆汤各剂量组细胞凋亡率则显著下降（

0.01）。与空白组比较，模型组细胞PI3K，Akt，GSK3

蛋白、磷酸化蛋白表达显著降低（

0.01）；与模型组比较，温胆汤各剂量组细胞PI3K，Akt，GSK3

蛋白、磷酸化蛋白表达明显升高（

0.05，

0.01）。与空白组比较，模型组细胞PI3K，Akt，GSK3

mRNA的表达显著降低（

0.01）；与模型组比较，温胆汤各剂量组细胞PI3K，Akt，GSK3

mRNA表达明显升高（

0.05，

0.01）。

结论

温胆汤含药血清可有效升高PI3K，Akt，GSK3

表达，从而调控PI3K/Akt/GSK3

信号通路，保护神经细胞。

Abstract

Objective

To investigate the protective effect of Wendantang-containing serum on astrocytes in glutamate environment and its effect on phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/glycogen synthetase kinase 3

(GSK3

) signal pathway.

Method

Totally 60 rats were randomly divided into 5 groups

normal group (

=20)

clozapine group

and high

medium and low-dose Wendantang groups

with 10 rats in each group. Normal group was given 20 mL·kg

-1

normal saline

clozapine group was given 20 mg·kg

-1

clozapine

Wendantang groups were given 40

10 g·kg

-1

Wendantang

once a day. Eight days later

the rats were killed

their blood was taken

serum was centrifuged

inactivated

filtrated

sterilized and filled in separate centrifugal tubes. The astrocytes were divided into normal group

model group

clozapine group

and high

medium and low-dose Wendantang groups. The normal group and the model group were cultured with normal serum

and the rest groups were cultured with corresponding drug containing serum. The other groups were treated with 10 mmol·L

-1

glutamic acid for 24 hours

and then the apoptosis of astrocytes was detected by flow cytometry. Western blot and Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) were used to determine protein

and mRNA expressions of PI3K

Akt

GSK3

in astrocytes.

Result

Compared with the normal group

apoptosis in model group increased significantly (

0.01). Compared with the model group

apoptosis in Wendantang groups decreased significantly (

0.01). Compared with the normal group

protein and phosphorylation expressions of PI3K

Akt

GSK3

in model groups decreased significantly (

0.05

0.01). Compared with the model group

protein and phosphorylation expressions of PI3K

Akt

GSK3

in Wendantang groups increased (

0.05

0.01). Compared with the normal group

expressions of PI3K

Akt and GSK3

mRNA decreased in model group(

0.01). Compared with the model group

expressions of PI3K

Akt and GSK3

mRNA increased significantly in Wendantang groups group (

0.05，

0.01).

Conclusion

Wendantang-containing serum can effectively increase expressions of PI3K

Akt and GSK3

so as to regulate PI3K/Akt/GSK3

signal pathway and protect nerve cells.

关键词

Keywords

references

朱金华 , 徐义勇 , 万红娇 , 等 . 温胆汤对精神分裂症模型大鼠海马组织PI3K，Akt和GSK3 β 的影响 [J]. 中国实验方剂学杂志 , 2019 , 25 ( 1 ): 101 - 106 .

刘婷婷 , 刘远新 . 从温胆汤看历代医家对痰病的论治及发挥 [J]. 新疆中医药 , 2009 , 27 ( 2 ): 8 - 11 .

陈超 . 温胆汤加减治疗癫狂症30例临床观察 [J]. 中医杂志 , 1984 , 11 : 33 - 34 .

姜建芳 , 范军铭 , 董永书 , 等 . 温胆汤加减治疗神经症临床进展 [J]. 中医研究 , 2008 , 21 ( 3 ): 62 - 64 .

朱金华 , 田真真 , 戎文娟 , 等 . 温胆汤对精神分裂症大鼠海马组织NRG1、ErbB4 mRNA表达及其行为学的影响 [J]. 中药药理与临床 , 2017 , 33 ( 3 ): 4 - 7 .

徐义勇 , 刘金莲 , 朱丽娟 , 等 . 温胆汤含药血清对谷氨酸环境下星型胶质细胞凋亡及NRG1、ErbB4表达的影响 [J]. 中药药理与临床 , 2019 , 35 ( 2 ): 7 - 11 .

朱金华 , 孙昊鑫 , 熊秋迎 , 等 . 温胆汤对精神分裂症模型鼠血清TNF- α ,IL-6及海马组织Glu活性表达的影响 [J]. 中国实验方剂学杂志 , 2014 , 20 ( 14 ): 160 - 164 .

朱金华 , 孙昊鑫 , 魏妍妍 , 等 . 温胆汤对精神分裂症模型鼠海马组织Cx43、谷氨酸及神经胶质细胞超微结构的影响 [J]. 中药药理与临床 , 2014 , 30 ( 5 ): 1 - 5 .

魏妍妍 , 刘丹丹 , 戎文娟 , 等 . 温胆汤对精神分裂症模型鼠海马PKC,p38MAPK及p-Cx43的影响 [J]. 中国实验方剂学杂志 , 2015 , 21 ( 11 ): 103 - 106 .

熊怀亮 , 朱金华 , 戎文娟 , 等 . 温胆汤含药血清对谷氨酸条件下星形胶质细Cx43和GJIC功能的影响 [J]. 中药药理与临床 , 2016 , 32 ( 1 ): 1 - 5 .

刘丹丹 , 魏妍妍 , 熊怀亮 , 等 . 温胆汤含药血清对谷氨酸条件下原代星形胶质细胞P38MAPK及PKC的影响 [J]. 中药药理与临床 , 2015 , 31 ( 1 ): 5 - 8 .

李扬 , 刘国良 , 姚远 , 等 . 杜仲总黄酮对小儿血管瘤内皮细胞凋亡的影响 [J]. 中国医药导报 , 2017 , 14 ( 24 ): 21 - 24 .

薛玉刚 , 程锦 , 曾广伟 , 等 . miR-214对过氧化氢诱导的心肌细胞凋亡的影响 [J]. 临床和实验医学杂志 , 2017 , 16 ( 17 ): 1692 - 1695 .

SZYDLOWSKA K , TYMIANSKI M . Calcium,ischemia and excitotoxicity [J]. Cell Calcium , 2010 , 47 ( 2 ): 122 - 129 .

陈世伟 , 张峰 , 张黎明 , 等 . 谷氨酸对神经细胞凋亡的影响 [J]. 山西医药杂志 , 2010 , 39 ( 9 ): 801 - 803 .

MANABE Y , WANG J M , MURAKAMI T , et al . Expressions of nitrotyrosine and TUNEL immunoreactivities in cultured rat spinal cord neurons after exposure to glutamate,nitric oxide,or peroxynitrite [J]. J Neurosci Res , 2001 , 65 ( 5 ): 371 - 377 .

YASUKO K , MAYUMI K , KANAE K , et al . Roles of PI3K/Akt/GSK3/mTOR pathway in cell signaling of mental illnesses [J]. Depress Res Treat , 2012 , doi: 10.1155/2012/752563 http://dx.doi.org/10.1155/2012/752563 .

AGUIAR J R A S , CASTRO A A , MOREIRA E L , et al . Short bouts of mild-intensity physical exercise improve spatial learning and memory in aging rats:involvement of hippocampal plasticity via Akt,CREB and BDNF signaling [J]. Mech Ageing Dev , 2011 , 132 ( 11 ): 560 - 567 .

ADACHI N , NUMAKAWA T , KUMAMARU E , et al . Phencyclidine-induced decrease of synaptic connectivity via inhibition of BDNF secretion in cultured cortical neurons [J]. Cerebral Cortex , 2013 , 23 ( 4 ): 847 - 858 .

王晅 , 朱燕珍 . 电针对血管性痴呆大鼠海马PI3K p85/Akt信号的影响 [J]. 山东中医药大学学报 , 2016 , 40 ( 5 ): 471 - 473 .

王娜 , 佟凤芝 , 曾常茜 . 姜黄素对海人酸致痫大鼠PI3K/Akt/mTOR通路调节作用的实验研究 [J]. 中国中医药科技 , 2015 , 22 ( 5 ): 534 - 536,549 .

徐少群 , 徐乐 , 李向宇 , 等 . 电针对异氟醚诱导阿尔茨海默样病小鼠海马区PI3K/Akt信号通路的影响 [J]. 吉林中医药 , 2016 , 36 ( 3 ): 294 - 297 .

毛小元 , 王志斌 , 周宏灏 , 等 . 蛇床子素通过激活PI3K/Akt通路减轻谷氨酸诱导的海马神经元损伤 [J]. 中南大学学报:医学版 , 2015 , 40 ( 9 ): 955 - 959 .

HERS I , VINCENT E E , TAVARÉ J M . Akt signalling in health and disease [J]. Cell Signal , 2011 , 23 ( 10 ): 1515 - 1527 .

ZHENG W , WANG H , ZENG Z , et al . The possible role of the Akt signaling pathway in schizophrenia [J]. Brain Res , 2012 , 1470 ( 35 ): 145 - 158 .

SHEN Y C , CHEN S F , CHEN C H , et al , Effects of DRD 2 /ANKK1 gene variations and clinical factors on aripiprazole efficacy in schizophrenic patients [J]. J Psychiatr Res, 2009 , 43 ( 6 ): 600 - 606 .

PILLAI A , BUCKLEY P F . Reliable biomarkers and predictors of schizophrenia and its treatment [J]. Psychiatr Clin North Am , 2012 , 35 ( 3 ): 645 - 659 .

THISELTON D L , VLADIMIROV V I , KUO P H , et al . Akt1 is associated with schizophrenia across multiple symptom dimensions in the Irish study of high density schizophrenia families [J]. Biol Psychiatry , 2008 , 63 ( 5 ): 449 - 457 .

SCHWAB S G , HOEFGEN B , HANSES C , et al . Further evidence for association of variants in the Akt1 gene with schizophrenia in a sample of European sibpair families [J]. Biol Psychiatry , 2005 , 58 ( 6 ): 446 - 450 .

EMAMIAN E S , HALL D , BIRNBAUM M J , et al . Convergent evidence for impaired Akt1-GSK3 β signaling in schizophrenia [J]. Nat Genet , 2004 , 36 ( 2 ): 131 - 137 .

AMAR S , SHALTIEL G , MANN L , et al . Possible involvement of post-dopamine D2 receptor signalling components in the pathophysiology of schizophrenia [J], Int J Neuropsychopharmacol , 2008 , 11 ( 2 ): 197 - 205 .

TAN H Y , CHEN A G , KOLACHANA B , et al . Effective connectivity of Akt1-mediated dopaminergic working memory networks and pharmacogenetics of anti-dopaminergic treatment [J]. Brain , 2012 , 135 ( 5 ): 1436 - 1445 .

TAN H Y , CHEN A G , CHEN Q , et al . Epistatic interactions of Akt1 on human medial temporal lobe biology and pharmacogenetic implications [J]. Mol Psychiatry , 2011 , 17 ( 10 ): 1007 - 1016 .

KRIVOV A , FISCHEL T , WEIZMAN A . The cognitive deficit in schizophrenia [J]. Harefuah , 2012 151 ( 5 ): 277 - 280,319 .

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Effect of Wendantang-containing Serum on Apoptosis and Expressions of PI3K，Akt and GSK3β in Astrocytes Under Glutamate Environment

DOI：10.13422/j.cnki.syfjx.20201304

摘要

Abstract

关键词

Keywords

references