Efficacy and Material Basis of Shuangjin Sanjie Granules in Treatment on Hyperplasia of Mammary Gland

Yu-cong BI; Yuan-yuan SHI; Lin ZHANG; Chun-lan FAN; Jia-lin LI; Dong-ling REN; Yan-tong XU; Chun-guo WANG; Ting WANG

doi:10.13422/j.cnki.syfjx.20201328

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Efficacy and Material Basis of Shuangjin Sanjie Granules in Treatment on Hyperplasia of Mammary Gland

Pharmacology | 更新时间：2020-08-11

- Efficacy and Material Basis of Shuangjin Sanjie Granules in Treatment on Hyperplasia of Mammary Gland
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 13, Pages: 111-117(2020)
- 作者机构：
  
  1. 北京中医药大学，北京 100029
  2. 国家中医药管理局名医名方重点研究室，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201328
  CLC： R22;R242;R2-031;R285.5
- Published Online：20 April 2020，
  
  Published：05 July 2020
- 稿件说明：
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毕于聪,史渊源,张林等.双金散结颗粒治疗乳腺增生的药效及物质基础[J].中国实验方剂学杂志,2020,26(13):111-117.

BI Yu-cong,SHI Yuan-yuan,ZHANG Lin,et al.Efficacy and Material Basis of Shuangjin Sanjie Granules in Treatment on Hyperplasia of Mammary Gland[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):111-117.
毕于聪,史渊源,张林等.双金散结颗粒治疗乳腺增生的药效及物质基础[J].中国实验方剂学杂志,2020,26(13):111-117. DOI： 10.13422/j.cnki.syfjx.20201328.

BI Yu-cong,SHI Yuan-yuan,ZHANG Lin,et al.Efficacy and Material Basis of Shuangjin Sanjie Granules in Treatment on Hyperplasia of Mammary Gland[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(13):111-117. DOI： 10.13422/j.cnki.syfjx.20201328.

摘要

目的

利用乳腺增生SD大鼠模型及超高效液相色谱-高分辨质谱联用（UHPLC-LTQ-Orbitrap MS）技术探讨双金散结颗粒治疗乳腺增生的药效及物质基础。

方法

将乳腺增生SD大鼠模型动物分组给药，测量乳头高度及直径，检测血清中雌二醇（E

），孕酮（P）及催乳素（PRL）含量，病理学检测乳腺组织增生情况，免疫组化检测雌激素受体

（ER

），雄激素受体（AR），孕酮受体（PR），肿瘤坏死因子-

（TNF-

）等蛋白表达，最后用UHPLC-LTQ-Orbitrap MS技术检测双金散结颗粒主要化学成分，与药效结合分析药效物质基础。

结果

动物实验结果显示，与正常组比较，模型组乳头高度增加，直径增宽（

0.01）；血清E

显著升高（

0.01）；病理学检测表明乳腺组织异常增生；ER

，AR，PR，TNF-

蛋白表达增加（

0.01）；与模型组比较，双金散结颗粒组乳头高度降低，直径减小（

0.01）；血清E

显著下降（

0.01）；病理学检测表明乳腺组织异常增生减弱；ER

，AR，PR，TNF-

蛋白表达显著降低（

0.01）。物质基础研究结果显示，从双金散结颗粒中共鉴定出85个化学成分，其中16种化学成分为生物碱类化合物，7种成分为黄酮类化合物，15种为萜类化合物，9种为酚酸类化合物，3种香豆素类化合物，10种酯类及内酯类化合物，7种脂肪酸类化合物，4种氨基酸类化合物和14种其他类成分，其中生物碱类及萜类成分可能与药效作用密切相关。

结论

双金散结颗粒可有效改善乳腺增生病变情况，其药效物质基础可能是柴胡皂苷a，d；贝母素甲、乙等成分。

Abstract

Objective

The SD rat model of hyperplasia of mammary gland（HMG） and the ultrahigh-performance liquid chromatography and mass spectrometry (UHPLC-LTQ-Orbitrap MS) technology were used to explore the pharmacological material basis of Shuangjin Sanjie granules (SJSJG) for the treatment on HMG.

Method

SD rat models of HMG were administered in groups

and the nipple height and the diameter were measured; the levels of estradiol (E

)

progesterone (P) and prolactin (PRL) in serum were detected

pathological examination was conducted for the hyperplasia of breast tissue. Histochemical methods were used to detect the expressions of estrogen receptor

(ER

)

androgen receptor (AR)

progesterone receptor (PR)

tumor necrosis factor-

(TNF-

) proteins. Finally

UHPLC-LTQ-Orbitrap MS technology was used to detect the main chemical constituents of SJSJG

and the pharmacodynamic substance basis was analyzed based on the pharmacological effect.

Result

The results of animal experiments showed that compared with the normal group

nipple height and diameter of the model group increased remarkably (

0.01)

serum E

significantly increased (

0.01). Pathological examination showed abnormal hyperplasia of breast tissue， expressions of ER

PR and TNF-

increased， compared with the model group

the nipple height and diameter of the SJSJG group decreased remarkably (

0.01)

serum E

was decreased significantly (

0.01)， pathological examination showed weakened abnormal hyperplasia of breast tissue

PR and TNF-

protein expressions were significant decreased (

0.01). The results of basic material study showed that 85 chemical components were identified from SJSJG

including 16 alkaloids

7 flavonoids

15 terpenes

9 phenolic acid compounds

3 coumarin compounds

10 esters and lactone compounds

7 fatty acids compounds

4 amino acids compounds

and 14 other types of ingredients， among them

alkaloids and terpenoids chemical drug substances were closely related.

Conclusion

SJSJG can effectively improve the condition of breast hyperplasia

and its medicinal substance basis may include saikosaponin A

Saikosaponin D

verticinone

peimine.

关键词

Keywords

references

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