Objective

2

To establish a mouse model of diminished ovarian reserve （DOR） induced by tripterygium wilfordii polyglycosides （TWP）， and to explore the therapeutic effect of Dingkundan （DKD） on DOR， so as to provide scientific basis for its clinical application.

Method

2

The 60 female Blab/c mice with regular estrous cycle were randomly divided into blank group， model group， low，medium and high-dose DKD group， DKD group and estradiol valerate group， with 10 mice in each group. Except the blank group， the other groups were given 40 mg·kg

-1

TWP suspension. Meantime，low，medium and high-dose DKD group were given 1.64，3.28，6.56 g·kg

-1

DKD suspension respectively， and estradiol valerate group was given 0.15 mg·kg

-1

estradiol valerate suspension by gastric lavage once a day for 30 days. The general condition， body weight， estrous cycle and gonad index of mice were observed， serum follicle-stimulating hormone （FSH）， luteinizing hormone （LH） and estradiol （E

2

） were determined by radioimmunoassay， ovarian morphology and follicle count were observed by hematoxylin-eosin staining （HE） staining.

Result

2

Compared with the blank group， most of the mice in model group had disordered estrous cycle， uterine and ovarian indexes decreased （

P

<

0.05）， serum FSH increased （

P

<

0.05）， LH was on an upward trajectory， E

2

was on a downward trend， and the number of growth follicles and corpus luteum decreased and the number of atresia follicles increased（

P

<

0.05）. Compared with the model group， half of the mice in DKD group resumed regular estrous cycle， however， the estrous cycles of mice in estradiol valerate group were stagnated during estrous period. In medium-dose， high-dose DKD group and estradiol valerate group， the uterine and ovarian indexes of the mice were increased， the serum FSH value decreased （

P

<

0.05） and serum LH was on a downward trend， high-dose DKD group and estradiol valerate group increased the levels of serum E

2

（

P

<

0.05）. In DKD group， the number of growth follicles and corpus luteum were increased and the number of atresia follicles were reduced （

P

<

0.05）， with the best effect at medium dosage. And in estradiol valerate group， the number of primitive follicles， sinusoidal follicles and corpus luteum were increased （

P

<

0.05）， but the number of atresia follicles had no difference to the model group.

Conclusion

2

DKD can improve serum sex hormones， promote follicular development and reduce follicular atresia， which can play a therapeutic role in the treatment of DOR.