Effect of Fushengong Prescription on p38 MAPK Signal Pathway of Rats with Chronic Renal Failure

Pan TONG; Xue-kuan HUANG; Qing SHEN; Yang ZHANG; Ke XU; Hong-yu LUO

doi:10.13422/j.cnki.syfjx.20201440

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Effect of Fushengong Prescription on p38 MAPK Signal Pathway of Rats with Chronic Renal Failure

Pharmacology | 更新时间：2020-09-09

- Effect of Fushengong Prescription on p38 MAPK Signal Pathway of Rats with Chronic Renal Failure
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 14, Pages: 105-110(2020)
- 作者机构：
  
  重庆医科大学中医药学院附属第一医院，重庆 400016
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201440
  CLC： R2-0;R285.5;R692
- Published Online：11 May 2020，
  
  Published：20 July 2020
- 稿件说明：
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童潘,黄学宽,沈清等.复肾功方对慢性肾衰竭大鼠p38 MAPK信号通路的影响[J].中国实验方剂学杂志,2020,26(14):105-110.

TONG Pan,HUANG Xue-kuan,SHEN Qing,et al.Effect of Fushengong Prescription on p38 MAPK Signal Pathway of Rats with Chronic Renal Failure[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):105-110.
童潘,黄学宽,沈清等.复肾功方对慢性肾衰竭大鼠p38 MAPK信号通路的影响[J].中国实验方剂学杂志,2020,26(14):105-110. DOI： 10.13422/j.cnki.syfjx.20201440.

TONG Pan,HUANG Xue-kuan,SHEN Qing,et al.Effect of Fushengong Prescription on p38 MAPK Signal Pathway of Rats with Chronic Renal Failure[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(14):105-110. DOI： 10.13422/j.cnki.syfjx.20201440.

摘要

目的

观察复肾功方对慢性肾衰竭（CRF）大鼠p38丝裂原活化蛋白激酶（p38 MAPK）信号通路的影响，探讨其减轻肾组织炎症反应及延缓肾间质纤维化发展的作用机制。

方法

将55只雄性SD大鼠随机分为正常组，模型组，复肾功方低、中、高剂量组，每组11只。正常组常规饲养，其余4组大鼠食用含0.5%腺嘌呤饲料制造CRF模型，连续造模21 d。造模成功后，所有大鼠改用常规饲料。正常组和模型组灌胃生理盐水20 mL·kg

-1

，复肾功方低、中、高剂量组分别以4，8，16 g·kg

-1

灌胃水煎剂，每日1次，连续30 d。实验结束后，Masson染色观察肾间质纤维化程度，免疫组化检测肾脏单核细胞趋化蛋白-1（MCP-1）的表达情况，蛋白免疫印迹法（Western blot）检测肾脏磷酸化p38丝裂原活化蛋白激酶（p-p38 MAPK）及转化生长因子-

（TGF-

）蛋白的表达情况。

结果

与正常组比较，模型组大鼠肾间质胶原沉积明显增多，肾脏组织MCP-1平均光密度值和p-p38 MAPK，TGF-

蛋白表达水平均明显升高（

0.05）；与模型组比较，复肾功方各剂量组肾间质胶原沉积明显减少，肾脏MCP-1平均光密度值及p-p38 MAPK，TGF-

蛋白表达水平均明显降低（

0.05）。

结论

复肾功方能有效抑制CRF大鼠肾脏组织相关炎症因子表达，从而减轻肾组织炎症反应，延缓肾间质纤维化的发展，其机制可能与抑制p38 MAPK信号通路的激活有关。

Abstract

Objective

To observe the effects of Fushengong prescription on p38 mitogen-activated protein kinase（p38 MAPK） signal pathway of rats with chronic renal failure（CRF），and to explore its mechanism of reducing inflammatory reaction of renal tissues and delaying the progress of renal interstitial fibrosis.

Method

The 55 male Sprague-Dawley rats were randomly divided into normal group，model group， and low，medium and high dose groups of Fushengong prescription，with 11 rats in each group.The normal group was routinely reared， and the other four groups of rats were fed a diet containing 0.5% adenine to produce a model of CRF， which was continuously molded for 21 days.After successful modeling，all rats switched to conventional feed.Normal group and model group were given normal saline 20 mL·kg

-1

，and each group of Fushengong prescription was given 4，8，16 g·kg

-1

of water prescription once a day for 30 days.After the experiment，Masson staining was used to observe the degree of renal interstitial fibrosis.The expression of monocyte chemotactic protein-1（MCP-1） in renal tissues was detected by immunohistochemistry. The expression of phosphorylated p38 mitogen-activated protein kinase（p-p38 MAPK） and transformed growth factor-

（TGF-

） in renal tissues were detected by Western blot.

Result

Compared with normal group，the renal interstitial collagen deposition increased significantly，the average optical density value of MCP-1 and the expression levels of p-p38 MAPK and TGF-

also increased significantly in model group （

0.05）. Compared with model group，the renal interstitial collagen deposition reduced significantly，the average optical density value of MCP-1 and the protein expression levels of p-p38 MAPK and TGF-

also decreased significantly in each dose group of Fushengong prescription（

0.05）.

Conclusion

Fushengong prescription can effectively inhibit the expression of related inflammatory factors in the renal tissue of CRF rats，so as to reduce the inflammatory response in the renal tissue and delay the progress of renal interstitial fibrosis，the mechanism of which may be related to inhibit the activation of p38 MAPK signal transduction pathway.

关键词

Keywords

references

韩海燕，路建饶，王新华 . 肾衰方改善早中期慢性肾衰竭患者肾功能及纤维化的临床观察 [J]. 中国实验方剂学杂志， 2016 ， 22 （ 15 ）: 166 - 170 .

ZHONG Y ， MENON M C ， DENG Y Y ， et al . Recent advances in traditional Chinese medicine for kidney disease [J]. Am J Kidney Dis ， 2015 ， 66 （ 3 ）: 513 - 522 .

黄学宽 . 郭子光临床经验集 [M]. 北京 : 人民卫生出版社， 2009 : 275 .

王玲，黄学宽，万磊，等 . 复肾功方对慢性肾功能衰竭大鼠肾脏 α -平滑肌肌动蛋白表达的影响 [J]. 中国临床药理学与治疗学， 2016 ， 21 （ 1 ）: 33 - 37 .

王玲，黄学宽，万磊，等 . 复肾功方对慢性肾功能衰竭大鼠肾脏Nephrin mRNA表达的影响 [J]. 四川大学学报:医学版， 2016 ， 47 （ 3 ）: 342 - 346 .

万磊，黄学宽，王玲，等 . 复肾功方对慢性肾功能衰竭大鼠肾功能及Shh信号通路的影响 [J]. 中医杂志， 2015 ， 56 （ 20 ）: 1771 - 1774 .

YANG Y ， WEI J M ， HUANG X K ， et al . iTRAQ-based proteomics of chronic renal failure rats after FuShengong Prescription treatment reveals haptoglobin and Alpha-1-Antitrypsin as potential biomarkers [J]. eCAM ， 2017 ， doi: 10.1155/2017/1480514 http://dx.doi.org/10.1155/2017/1480514 .

LIM A K H ， TESCH G H . Inflammation in diabetic nephropathy [J]. Mediators Inflamm ， 2012 ， doi: 10.1155/2012/146154 http://dx.doi.org/10.1155/2012/146154 .

LIU Y . Cellular and molecular mechanisms of renal fibrosis [J]. Nat Rev Nephrol ， 2011 ， 7 （ 12 ）: 684 - 696 .

梁亮，王圣志，何学红 . 参芪泄浊饮对腺嘌呤致慢性肾衰大鼠模型肾组织细胞外基质表达影响 [J]. 辽宁中医药大学学报， 2015 ， 17 （ 1 ）: 27 - 29 .

魏伟 . 药理实验方法学 [M]. 4版 . 北京 : 人民卫生出版社， 2010 : 69 - 73 .

WEBSTER A C ， NAGLER E V ， MORTON R L ， et al . Chronic kidney disease [J]. Lancet ， 2017 ， 389 （ 10075 ）: 1238 - 1252 .

康阳阳 . 中国成人慢性肾脏病患病率Meta分析 [D]. 郑州 : 郑州大学， 2017 : 6 .

ZHONG J Y ， YANG H C ， FOGO A B . A perspective on chronic kidney disease progression [J]. Am J Physiol Renal Physiol ， 2017 ， 312 （ 3 ）: F375 -F384.

梅全喜 . 简明实用中药药理手册 [M]. 北京 : 人民卫生出版社， 2010 .

SATO Y K ， YANAGITA M . Resident fibroblasts in the kidney: a major driver of fibrosis and inflammation [J]. Inflamm Regen ， 2017 ， 37 : 17 .

YANG Y ， SONG M F ， LIU Y ， et al . Renoprotective approaches and strategies in acute kidney injury [J]. Pharmacol Ther ， 2016 ， 163 : 58 - 73 .

DECLÈVES A E ， SHARMA K . Novel targets of antifibrotic and anti-inflammatory treatment in CKD [J]. Nat Rev Nephrol ， 2014 ， 10 （ 5 ）: 257 - 267 .

CASSIDY H ， RADFORD R ， SLYNE J ， et al . The role of MAPK in drug-induced kidney injury [J]. J Signal Transduct ， 2012 ， doi: 10.1155/2012/463617 http://dx.doi.org/10.1155/2012/463617 .

黄燕如，万毅刚，孙伟，等 . 雷公藤多苷调节肾组织p38 MAPK信号通路改善糖尿病肾病肾小球炎症性损伤的作用和机制 [J]. 中国中药杂志， 2014 ， 39 （ 21 ）: 4102 - 4109 .

XU L H ， SHEN P Q ， BI Y L ， et al . Danshen injection ameliorates STZ-induced diabetic nephropathy in association with suppression of oxidative stress，pro-inflammatory factors and fibrosis [J]. Int Immunopharmacol ， 2016 ， 38 : 385 - 394 .

陈建，曾莉，何立群，等 . 从巨噬细胞角度研究肾纤维化发病机制进展 [J]. 中国中西医结合肾病杂志， 2015 ， 16 （ 7 ）: 639 - 641 .

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