Effect of Ultrafine Powder of Gastrodiae Rhizoma in Improving Learning and Memory Ability of Vascular Dementia Rats by Regulating Cholinergic System

Ting-ting CHEN; Xue ZHOU; Yi-ni XU; Fei YANG; Xiao-ying WU; Rui RAN; Quan XIANG; Bin LIANG; Ling TAO; Xiang-chun SHEN

doi:10.13422/j.cnki.syfjx.20201503

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Effect of Ultrafine Powder of Gastrodiae Rhizoma in Improving Learning and Memory Ability of Vascular Dementia Rats by Regulating Cholinergic System

Pharmacology | 更新时间：2020-08-11

- Effect of Ultrafine Powder of Gastrodiae Rhizoma in Improving Learning and Memory Ability of Vascular Dementia Rats by Regulating Cholinergic System
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 15, Pages: 26-32(2020)
- 作者机构：
  
  1.贵州医科大学天然药物优效利用重点实验室，贵州省高等学校天然药物药理与成药性评价特色重点实验室，贵阳 550025
  2.贵州威门制药有限公司，贵阳 550025
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201503
  CLC： R2-0;R22;R285.5
- Revised：22 November 2019，
  
  Published Online：08 May 2020，
  
  Published：05 August 2020
- 稿件说明：
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陈婷婷,周雪,徐旖旎等.天麻超微粉调控胆碱能系统改善血管性痴呆大鼠学习记忆能力[J].中国实验方剂学杂志,2020,26(15):26-32.

CHEN Ting-ting,ZHOU Xue,XU Yi-ni,et al.Effect of Ultrafine Powder of Gastrodiae Rhizoma in Improving Learning and Memory Ability of Vascular Dementia Rats by Regulating Cholinergic System[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(15):26-32.
陈婷婷,周雪,徐旖旎等.天麻超微粉调控胆碱能系统改善血管性痴呆大鼠学习记忆能力[J].中国实验方剂学杂志,2020,26(15):26-32. DOI： 10.13422/j.cnki.syfjx.20201503.

CHEN Ting-ting,ZHOU Xue,XU Yi-ni,et al.Effect of Ultrafine Powder of Gastrodiae Rhizoma in Improving Learning and Memory Ability of Vascular Dementia Rats by Regulating Cholinergic System[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(15):26-32. DOI： 10.13422/j.cnki.syfjx.20201503.

摘要

目的

天麻超微粉（UPG）是否可以改善血管性痴呆（VD）大鼠的学习记忆功能障碍并且延缓VD的进程，该作用是否与乙酰胆碱酯酶（AChE）和胆碱乙酰转移酶（ChAT）调节乙酰胆碱（Ach）的释放从而调控胆碱能系统有关。

方法

将SD大鼠随机分出假手术组，其余大鼠通过永久性大脑中动脉闭塞法（pMCAO）复制VD大鼠模型，并随机分为模型组，天麻超微粉低剂量组（0.45 g·kg

-1

），天麻超微粉高剂量组（1.8 g·kg

-1

），石杉碱甲组（80 μg·kg

-1

），每组各12只。给药组每天灌胃1次，持续8周，假手术组和模型组给予等量蒸馏水灌胃后，通过Morris水迷宫实验对各组大鼠的学习和记忆能力进行评估；苏木素-伊红（HE）染色对大鼠海马CA1区进行病理形态学观察；酶联免疫吸附测定（ELISA）检测大鼠海马组织中Ach的水平；蛋白免疫印迹法检测大鼠海马组织中AChE，ChAT的蛋白表达；免疫组化实验观察海马组织CA1区ChAT的表达情况。

结果

与假手术组比较，模型组的逃避潜伏期增加，穿过平台的次数和在目标象限停留的时间显著减少（

0.01），模型组海马CA1区神经细胞排列无序，细胞核固缩且胞浆颜色较深，坏死细胞数量增多，海马组织中Ach的水平显著下降（

0.01），AChE蛋白表达水平显著上调（

0.01），ChAT蛋白表达水平明显下调（

0.05）；与模型组比较，各给药组能够显著降低模型大鼠的逃避潜伏期，显著增加穿过平台的次数和在目标象限停留的时间（

0.01），神经细胞排列趋于整齐，细胞核呈圆形且胞浆染色浅，坏死细胞减少，海马组织中Ach水平明显增加（

0.05），AChE蛋白表达显著下调，ChAT蛋白表达显著上调（

0.01）。

结论

天麻超微粉能够改善血管性痴呆大鼠的学习记忆障碍，其作用机制与上调ChAT蛋白，抑制AChE蛋白，提高脑内乙酰胆碱的作用密切相关。

Abstract

Objective

To investigate whether ultrafine powder of Gastrodiae Rhizoma （UPG） can alleviate the learning and memory impairment of vascular dementia rats and delay the process of VD， and whether this effect is related to the release of acetylcholine （Ach） through the regulation with acetylcholinesterase （AChE） and choline acetyltransferase （ChAT） and control of cholinergic system.

Method

SD rats were randomly divided into sham operation group， UPG low dose group （0.45 g·kg

-1

）， UPG high dose group （1.8 g·kg

-1

） and Huperzine A group （80 μg·kg

-1

）， with 12 rats in each group. The drug administration groups were given orally drugs once a day for 8 weeks， and sham group and model group were given orally the same amount of distilled water. The learning and memory ability of the rats with VD were evaluated by the Morris water maze. Htoxylin eosin（HE） staining was used for pathomorphological observation of hippocampus CA1 area of the rats. The content of Ach was determined by enzyme-linked immunosorbent assay（ELISA）， AChE and ChAT protein expressions were detected by Western blot， and expression of ChAT in hippocampus CA1 area was observed by immunohistochemistry.

Result

Compared with the sham operation group， the escape latency of the model group was significantly increased （

0.01）， and the frequency of crossings platform and the time of staying in the target quadrant were reduced significantly （

0.01）. HE staining of hippocampal tissues from VD rat showed neuron disorders， loss and degeneration and necrosis， pyknosis of the nucleus and light coloration of the cytoplasm. The level of acetylcholine in the hippocampus was significantly decreased by ELISA （

0.05）， the expression level of AChE protein was significantly up-regulated， and the expression level of ChAT protein was significantly down-regulated （

0.01）. Compared with model group， each administration group could significantly reduce the escape latency of the model rats， and significantly increase the frequency of crossing platform and the time of staying in the target quadrant （

0.01）， the content of Ach was significantly increased （

0.05）， the expression of AChE protein was significantly down-regulated （

0.01）， while the expression of ChAT protein was significantly up-regulated （

0.01）.

Conclusion

UPG improves the learning and memory ability of vascular dementia rats， and its mechanism may be related to the increase of Ach， ChAT level and the decrease of AChE level.

关键词

Keywords

references

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