Mechanism of Bushen Huayu Shengxin Decoction in Delaying Senescence of Bone Marrow Mesenchymal Stem Cells by Improving Ischemic-hypoxic Microenvironment

ZHANG Bao-xia; ZHANG Jin-sheng; HUI Xiao-shan; ZHANG Li-na

doi:10.13422/j.cnki.syfjx.20201604

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Mechanism of Bushen Huayu Shengxin Decoction in Delaying Senescence of Bone Marrow Mesenchymal Stem Cells by Improving Ischemic-hypoxic Microenvironment

更新时间：2023-07-12

- Mechanism of Bushen Huayu Shengxin Decoction in Delaying Senescence of Bone Marrow Mesenchymal Stem Cells by Improving Ischemic-hypoxic Microenvironment
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 16, Pages: 87-92(2020)
- 作者机构：
  
  河南中医药大学第一附属医院，郑州 450000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201604
  CLC： R2-0;R22;R285.5
- Published Online：09 June 2020，
  
  Published：20 August 2020
- 稿件说明：
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张宝霞,张金生,惠小珊等.补肾化瘀生新方改善缺血缺氧性微环境延缓骨髓间充质干细胞衰老的作用[J].中国实验方剂学杂志,2020,26(16):87-92.

ZHANG Bao-xia,ZHANG Jin-sheng,HUI Xiao-shan,et al.Mechanism of Bushen Huayu Shengxin Decoction in Delaying Senescence of Bone Marrow Mesenchymal Stem Cells by Improving Ischemic-hypoxic Microenvironment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(16):87-92.
张宝霞,张金生,惠小珊等.补肾化瘀生新方改善缺血缺氧性微环境延缓骨髓间充质干细胞衰老的作用[J].中国实验方剂学杂志,2020,26(16):87-92. DOI： 10.13422/j.cnki.syfjx.20201604.

ZHANG Bao-xia,ZHANG Jin-sheng,HUI Xiao-shan,et al.Mechanism of Bushen Huayu Shengxin Decoction in Delaying Senescence of Bone Marrow Mesenchymal Stem Cells by Improving Ischemic-hypoxic Microenvironment[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(16):87-92. DOI： 10.13422/j.cnki.syfjx.20201604.

摘要

目的

观察补肾化瘀生新方改善细胞生存的不同微环境对p16/pRb和p53/p21信号通路调控作用，探讨其对骨髓间充质干细胞（BMSCs)衰老的影响。

方法

采用无血清的1640培养基和低氧细胞工作站培养24 h建立缺血缺氧微环境细胞体外模型。随机分为空白组，模型组，补肾化瘀生新方组（24.58 g·kg

-1

），复合培养基组；复合培养基组加入复合培养基，模型组完全培养基培养；补肾化瘀生新方组加入含有补肾化瘀生新方的血清培养基，均在低氧细胞工作站培养24 h；空白组加入完全培养基正常培养；采用流式细胞仪检测BMSCs细胞周期、实时荧光定量聚合酶链式反应（Real-time PCR）检测p16

INK4a

，p53，p21 mRNA表达、及存活素（Survivn），半胱氨酸天冬氨酸蛋白酶-3（Caspase-3），聚腺苷二磷酸核糖聚合酶（PARP） mRNA表达；蛋白免疫印迹法（Western blot）检测

-catenin和糖原合成酶激酶-3

（GSK-3

）蛋白的表达。

结果

与空白组比较，模型组S期细胞数增多，G

期明显降低，p16

INK4a

，p53，p21，Survivn，Caspase-3，PARP mRNA表达明显升高，

-catenin和GSK-3

蛋白表达明显升高（

0.05）；与模型组比较，补肾化瘀生新方组S期细胞数减少，G

期比值明显升高（

0.05），p16

INK4a

，p53，p21，Survivn，Caspase-3，PARP mRNA表达明显降低，

-catenin和GSK-3

蛋白表达明显降低（

0.05）。

结论

补肾化瘀生新方通过改善缺血缺氧性微环境调控p16/pRb和p53/p21信号通路延缓BMSCs衰老。

Abstract

Objective

To explore the mechanism of Bushen Huayu Shengxin decoction in delaying senescence of bone mesenchymal stem cells(BMSCs) by improving cellular microenvironment and regulating p16/pRb and p53/p21 signaling pathways.

Method

The cells were cultured in serum-free 1640 medium and hypoxic cell workstation for 24 hours to establish the cell model of ischemic-hypoxic microenvironment in vitro

then randomized into control group (with complex medium)

model group (with complete medium)

and treatment group (with serum medium-containing Bushen Huayu Shengxin decoction)

and all were cultured in hypoxic cell workstation for 24 hours. The normal group was added with control culture for complete medium

The cell cycle of BMSCs was detected by flow cytometry

the expressions of p16

INK4a

p53

p21 and Survivn

cysteine aspartic acid protease-3 (Caspase-3)

polyadenosine diphosphate ribose polymerase (PARP) mRNA were analyzed by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR)

and the levels of

-catenin protein and glycogen synthase kinase-3

（GSK-3

） protein were detected by Western blot.

Result

Compared with the normal group

the proportion of S phase cells increased

while that at the G

phase decreased significantly in the model group (P

0.05). Compared with the model group

the proportion of S phase decreased

whereas that at the G

phase gradually increased in the treatment group (

0.05). Compared with the normal group

mRNA expressions of p16

INK4a

p53

p21 and Survivn

Caspase-3

PARP in the model group increased significantly (

0.05). Compared with the model group

mRNA expressions of p16

INK4a

p53

p21 and Survivn

Caspase-3

PARP in the treatment group decreased significantly (

0.05). Compared with the normal group

protein expressions of

-catenin and GSK-3

in the model group increased significantly (

0.05). Compared with the model group

protein expressions of

-catenin and GSK-3

in the treatment group decreased significantly (

0.05).

Conclusion

Bushen Huayu Shengxin decoction could delay the senescence of BMSCs by improving ischemic-hypoxic microenvironment and regulating p16/pRb and p53/p21 signaling pathways.

关键词

Keywords

references

HAJINEJAD M , PASBAKHSH P , OMIDI A , et al . esveratrol pretreatment enhanced homing of SDF-1 α -preconditioned bone marrow-derived mesenchymal stem cells in a rat model of liver cirrhosis [J]. J Cell Biochem , 2017 , 11 : 1000 - 1002 .

汪文请，陈旭听，韩志海 . 干细胞衰老机制及应对策略的研究进展 [J]. 转化医学杂志 , 2016 ， 5 ( 4 ): 253 - 256 .

ZHANG B X , ZHANG J S , DU M M , et al . Chinese preparation uesaitong promotes the mobilization of bone narow masenchoimal stem cells in rats with cerebral [J]. Neural Regener Res ， 2016 , 11 ( 2 ): 292 - 297 .

张宝霞 , 张金生 , 张阳阳 , 等 . 三七总皂苷、红景天疳、黄芪有效组分对心肌梗死大鼠模型骨髓间充质干细胞动员作用研究 [J]. 中国实验方剂学杂志 , 2016 , 22 ( 8 ): 137 - 142 .

苗明三 . 实验动物和动物实验技术 [M]. 北京 : 中国中医药出版社 , 2003 : 61 - 63 .

邢衢 , 马珊珊 , 王欣欣 , 等 . 人脐带间充质干细胞神经分化微环境的优化 [J]. 郑州大学学报:医学版 , 2016 , 51 ( 4 ): 469 - 474 .

张妮 , 陈兰英 , 李雪亮 , 等 . 炎症诱导缺血缺氧环境下骨髓间充质干细胞的活性 [J]. 中国组织工程研究 . 2018 , 22 ( 33 ): 5259 - 5267 .

GONZALEZ M E , MARTIN E E , ANWAR T , et al . Mesenchymal stem cell-induced ddr2 mediates stromal-breast cancer interactions and metastasis growth [J]. Cell Rep , 2017 , 18 ( 5 ): 1215 - 1228 .

KHONGD , LI M , SINGLETON A , et al . Orthogonal potency analysis of mesenchymalstromal cell function during ex vivo expansion [J]. Exp Cell Res , 2017 , 4827 ( 17 ): 30610 - 30619 .

WANG L , CHEN K , WAN X , et al . NLRP3 inflammasome activation in mesenchymal stem cells inhibits osteogenic differentiation and enhances adipogenic differentiation [J]. Biochem Biophys Res Commun , 2017 , 484 ( 4 ): 871 - 877 .

SON H E , KIM E J , JANG W G . Curcumin induces osteoblast differentiation through mild-endoplasmic reticulum stress-mediated such as BMP2 on osteoblast cells [J]. Life Sci , 2018 , 193 : 34 - 39 .

赖小华 , 雷燕 , 杨静 , 等 . 基于microRNA-34a/SIRT1/p53通路探讨三七皂苷R 1 对血管内皮细胞衰老的影响 [J]. 中国中药杂志 , 2018 , 43 ( 3 ): 577 - 584 .

曹东维 , 韩文贝 , 何劲松 , 等 . 茶多酚调节STAT3/miR-126/端粒信号通路活性延缓高糖诱导的人肾小球系膜细胞衰老 [J]. 中国中药杂志 , 2018 , 43 ( 23 ): 4678 - 4684 .

张宝霞 , 张金生 , 许二平 , 等 . 补肾化瘀生新法协同静脉注射骨髓间充质干细胞对延缓大鼠衰老作用 [J]. 中国实验方剂学杂志 , 2018 , 24 ( 24 ): 116 - 126 .

张金生，张宝霞 . 机体衰老、干细胞衰老与补肾化瘀生新 [J]. 中医研究 , 2015 , 28 ( 12 ): 1 - 3 .

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