Mechanism of Shengmaiyin (Dangshen Prescription) in Treatment of Atherosclerotic Cardiovascular Disease Based on Network Pharmacology

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Mechanism of Shengmaiyin (Dangshen Prescription) in Treatment of Atherosclerotic Cardiovascular Disease Based on Network Pharmacology

Data Mining | 更新时间：2020-09-09

- Mechanism of Shengmaiyin (Dangshen Prescription) in Treatment of Atherosclerotic Cardiovascular Disease Based on Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 17, Pages: 151-161(2020)
- 作者机构：
  
  1.山西医科大学药学院，太原 030001
  2.中国中医科学院中药研究所，北京 100700
  3.广东药科大学中药学院，广州 510006
  4.山西医科大学第一医院，太原 030001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201706
  CLC： R2-0;R22;R285.5;R289
- Published Online：28 June 2020，
  
  Published：05 September 2020
- 稿件说明：
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杨婷,陈利娜,瞿水清等.基于网络药理学的生脉饮（党参方）治疗动脉粥样硬化性心血管疾病的潜在分子机制[J].中国实验方剂学杂志,2020,26(17):151-161.

YANG Ting,CHEN Li-na,QU Shui-qing,et al.Mechanism of Shengmaiyin (Dangshen Prescription) in Treatment of Atherosclerotic Cardiovascular Disease Based on Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):151-161.
杨婷,陈利娜,瞿水清等.基于网络药理学的生脉饮（党参方）治疗动脉粥样硬化性心血管疾病的潜在分子机制[J].中国实验方剂学杂志,2020,26(17):151-161. DOI： 10.13422/j.cnki.syfjx.20201706.

YANG Ting,CHEN Li-na,QU Shui-qing,et al.Mechanism of Shengmaiyin (Dangshen Prescription) in Treatment of Atherosclerotic Cardiovascular Disease Based on Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):151-161. DOI： 10.13422/j.cnki.syfjx.20201706.

摘要

目的

2

运用网络药理学方法，研究生脉饮（党参方）治疗动脉粥样硬化性心血管病（ASCVD）的活性成分、作用靶点和分子通路，揭示生脉饮（党参方）治疗ASCVD的分子机制，并为组方配伍的合理性阐释提供依据。

方法

2

借助中西医结合数据库（SymMap），中药系统药理学技术平台（TCMSP）以及BATMAN-TCM平台获取生脉饮（党参方）主要化学成分，SymMap和中医药百科网站（ETCM）检索化合物靶点，借助疾病相关的基因与突变位点数据库（DisGeNET）和基因组注释（GeneCards）数据库检索疾病靶点；化合物靶点与疾病靶点作交集得到生脉饮（党参方）作用于ASCVD的预测靶点。利用蛋白质相互作用（STRING）数据库构建靶蛋白相互作用（PPI）网络图，借助Cytoscape软件获取生脉饮（党参方）作用于ASCVD的关键化合物和关键靶点，最后用The Database for Annotation，Visualization and Integrated Discovery（DAVID）网站将富集到的关键靶点进行基因本体（GO）生物学过程分析和京都基因与基因组百科全书（KEGG）通路分析。

结果

2

生脉饮（党参方）作用于ASCVD的关键化合物有33个，关键靶点有25个。GO分析结果显示，生脉饮（党参方）治疗ASCVD关键靶点的生物功能主要涉及凋亡过程的调控、炎症反应、一氧化氮合成的调节、胰岛素分泌的调节等生物学过程；KEGG通路主要富集到磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）信号通路、凋亡信号通路、肿瘤坏死因子（TNF）信号通路、雌激素信号通路等20条信号通路。

结论

2

本研究通过网络药理学从分子水平探讨生脉饮（党参方）治疗ASCVD的活性成分与潜在靶点，初步验证了生脉饮（党参方）的作用机制，并为进一步深入探究其作用机制奠定了理论基础。

Abstract

Objective

2

By means of network pharmacology

the active ingredients

targets and molecular pathways of Shengmaiyin (Dangshen prescription) in the treatment of atherosclerotic cardiovascular disease (ASCVD) were studied

in order to reveal the molecular mechanism of Shengmaiyin (Dangshen prescription) in the treatment of ASCVD

and provide the rational explanation of the compatibility of the combination.

Method

2

The main chemical components of Shengmaiyin (Dangshen prescription) were obtained by means of SymMap database

traditional Chinese medicine systems pharmacology database and analysis platform（TCMSP）platform and BATMAN-TCM platform. Compound targets were retrieved by SymMap and the Encyclopedia of Traditional Chinese Medicine （ETCM）

and disease targets were retrieved by DisGeNET and GeneCards databases. The intersections of compound targets and disease targets were used to obtain the predicted targets of song-decoction on ASCVD. The Protein-Protein Interaction (PPI) network diagram was constructed through STRING database

and key compounds and targets of Shengmaiyin (Dangshen prescription) acting on ASCVD were obtained through Cytoscape. Finally

the enriched key targets were put for Gene Ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis through the Database for Annotation，Visualization and Integrated Discovery（DAVID）.

Result

2

There were 33 key compounds and 25 key targets of Shengmaiyin (Dangshen prescription) for ASCVD. The GO analysis showed that the biological functions of Shengmaiyin (Dangshen prescription) in the treatment of key ASCVD targets mainly involved biological processes

such as the regulation of apoptosis

inflammatory response

regulation of nitric oxide synthesis and regulation of insulin secretion. The KEGG pathway was mainly enriched in 20 signaling pathways

including tumor necrosis factor(TNF) signaling pathway

phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt) signaling pathway

apoptosis signaling pathway and estrogen signaling pathway.

Conclusion

2

Through network pharmacology

this study explored active ingredients and potential targets of Shengmaiyin (Dangshen prescription) in the treatment of ASCVD at the molecular level

preliminarily verified the mechanism of action of Shengmaiyin (Dangshen prescription)

and laid a theoretical foundation for further study on the mechanism of action.

关键词

Keywords

references

中华心血管病杂志编辑委员会 . 中国心血管病预防指南(2017) [J]. 中华心血管病杂志 , 2018 , 46 ( 1 ): 10 - 25 .

ZHOU M , WANG H , ZENG X , et al . Mortality,morbidity,and risk factors in China and its provinces,1990—2017: a systematic analysis for the Global Burden of Disease Study 2017 [J]. Lancet , 2019 , 394 ( 10204 ): 1145 - 1158 .

曹占鸿 , 潘建衡 , 李娜 , 等 . 生脉散现代药理作用及作用机制的研究进展 [J]. 中国实验方剂学杂志 , 2019 , 25 ( 22 ): 212 - 218 .

黄青萍 . 生脉饮中人参与党参的薄层鉴别 [J]. 广西中医药 , 2004 , 27 ( 1 ): 57 .

楚楚 , 徐绍静 , 吴琼琼 , 等 . HPLC同时测定生脉饮(党参方)中多组分的含量 [J]. 中药材 , 2013 ( 5 ): 820 - 823 .

徐丽萍 , 裴妙荣 , 杨翠玲 , 等 . 生脉散分析方法的研究进展 [J]. 山西职工医学院学报 , 2007 , 17 ( 2 ): 77 - 78 .

杜玉杰 , 陶永 . 生脉饮加味治疗心律失常4则 [J]. 中医药临床杂志 , 2012 , 24 ( 9 ): 894 .

张晨 , 王哲 , 徐强 , 等 . 生脉方及其主要药物治疗心血管病药理作用研究概况 [J]. 中西医结合心脑血管病杂志 , 2018 , 16 ( 14 ): 51 - 53 .

程艳刚 , 李国艳 , 谭金燕 , 等 . 黄酮类化合物抗心肌缺血作用机制研究进展 [J]. 辽宁中医药大学学报 , 2018 , 20 ( 6 ): 82 - 85 .

万蕾 . Caspase 3表达调控在木犀草素抗大鼠离体心脏A/R损伤中作用机制研究 [D]. 南昌 : 南昌大学 , 2018 .

BASU A , DAS A S , MAJUMDER M , et al . Antiatherogenic roles of dietary flavonoids chrysin,quercetin,and luteolin [J]. J Cardiovasc Pharmacol , 2016 , 68 ( 1 ): 89 - 96 .

LU W , KANG J , HU K , et al . The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia [J]. Sleep Breath , 2017 , 149 ( 4 ): A581 .

LIN M , WEI S , WAN G , et al . Methylophiopogonanone a protects against cerebral ischemia/reperfusion injury and attenuates blood-brain barrier disruption in vitro [J]. PLoS One , 2015 , 10 ( 4 ): e124558 .

WANG A Y , LIU B F , LIANG A Z , et al . Homoisoflavonoids and the antioxidant activity of Ophiopogon japonicus root [J]. Iran J Pharm Res , 2017 , 16 ( 1 ): 357 - 365 .

CSUPOR D , WIDOWITZ U , BLAZSÓ G , et al . Anti-inflammatory activities of eleven centaurea species occurring in the carpathian basin [J]. Phytother Res , 2013 , 27 ( 4 ): 540 - 544 .

NIA A M , KALANTARIPOUR T P , BASIRI M , et al . Nepeta dschuparensis bornm extract moderates COX-2 and IL-1 β proteins in a rat model of cerebral ischemia [J]. Iran J Med Sci , 2017 , 42 ( 2 ): 179 - 186 .

刘盈盈 , 易兴阳 , 王淳 , 等 . COX基因多态性与缺血性卒中的相关性研究 [J]. 西部医学 , 2019 , 31 ( 6 ): 883 - 888 .

卢长青 , 贾合磊 , 雷震 , 等 . 五味子乙素通过诱导线粒体自噬减轻心肌缺血再灌注损伤 [J]. 安徽医科大学学报 , 2019 , 54 ( 3 ): 86 - 90 .

PAPAVASSILIOU A G , TOUSOULIS D , SIASOS G , et al . Vascular inflammation and atherosclerosis: the role of estrogen receptors [J]. Curr Med Chem , 2015 , 22 ( 22 ): 2651 - 2665 .

陈凡 , 叶俊梅 , 张玉彬 . Akt在心血管疾病中的调控作用 [J]. 药学研究 , 2018 , 37 ( 5 ): 291 - 294,297 .

吴青华 , 李冰涛 , 朱水兰 , 等 . 蒙古族药芯芭治疗2型糖尿病的网络药理学研究 [J]. 中国中药杂志 , 2020 , 45 ( 8 ): 1764 - 1771 .

饶小娟 , 吴毓敏 , 王彦 . 麦冬皂苷介导AMPK/Akt/mTOR通路对糖尿病大鼠心肌细胞自噬的影响 [J]. 中国循证心血管医学杂志 , 2018 , 10 ( 11 ): 1362 - 1367 .

HU B , LIU C , PARK D . Alteration of MAP kinase pathways after transient forebrain ischemia [J]. J Cereb Blood Flow Metab , 2000 , 20 ( 7 ): 1089 - 1095 .

LIU X , TAN W , YANG F , et al . Shengmai injection reduces apoptosis and enhances angiogenesis after myocardial ischaemia and reperfusion injury in rats [J]. Biomed Pharmacother , 2018 , 104 : 629 - 636 .

INSERTE J , CARDONA M , PONCELAS-NOZAL M , et al . Studies on the role of apoptosis after transient myocardial ischemia:genetic deletion of the executioner caspases-3 and -7 does not limit infarct size and ventricular remodeling [J]. Basic Res Cardiol , 2016 , 111 ( 2 ): 18 .

曹斌 , 于梅 . 麦冬皂苷D对大鼠心肌细胞凋亡及caspase通路的影响 [J]. 中国药师 , 2019 , 22 ( 3 ): 451 - 456 .

CHANG R , LI Y , YANG X , et al . Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats [J]. PLoS One , 2013 , 8 ( 4 ): e61590 .

CANTLEY L C . The phosphoinositide 3-kinase pathway [J]. Science , 2002 , 296 ( 5573 ): 1655 - 1657 .

陆定艳 , 李靖 , 孙佳 , 等 . 参芎葡萄糖注射液通过激活PI3K/AKT通路拮抗H 2 O 2 诱导H9c2细胞凋亡 [J]. 中国中药杂志 , 2019 , 44 ( 17 ): 3773 - 3779 .

聂阳 , 丁立 , 黄海潮 , 等 . PI3K/Akt-eNOS信号通路在甘木通总黄酮后处理减轻大鼠心肌缺血再灌注损伤作用 [J]. 中国中药杂志 , 2018 , 43 ( 23 ): 4692 - 4697 .

罗俊华 , 姜辉 , 吴娜 , 等 . 生脉注射液在高龄感染性休克患者中的应用价值及对ET-1、NO表达的影响 [J]. 临床军医杂志 , 2014 , 42 ( 9 ): 890 - 893 .

HE F , XU B L , CHEN C , et al . Methylophiopogonanone A suppresses ischemia/reperfusion-induced myocardial apoptosis in mice via activating PI3K/Akt/eNOS signaling pathway [J]. Acta Pharmacol Sin , 2016 , 37 ( 6 ): 763 - 771 .

孙晓莉 , 郑雪冰 , 王蕊 , 等 . 生脉注射液对心肺复苏中心肌再灌注损伤的保护作用 [J]. 中国实验诊断学 , 2015 , 19 ( 8 ): 96 - 98 .

WANG L , LU W , GAO Y H , et al . Anluohuaxianwan Alleviates carbon tetrachloride-induced hepatic fibrosis in rats through upregulation of peroxisome proliferator‑activated receptor-gamma and downregulation of nuclear factor-kappa B/IκBα signaling pathway [J]. World J Tradit Chin Med , 2019 , 5 ( 2 ): 95 - 103 .

GU J , LIU G , XING J , et al . Fecal bacteria from Crohn's disease patients more potently activated NOD-like receptors and Toll-like receptors in macrophages,in an IL-4-repressible fashion [J]. Microb Pathog , 2018 , 121 : 40 - 44 .

TIAN X , LIU C , SHU Z , et al . Review:therapeutic targeting of HMGB1 in stroke [J]. Curr Drug Deliv , 2017 , 14 ( 6 ): 785 - 790 .

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