Effect of Euphorbiae Ebarcteolatae Radix Bacteriostasis Ointment on Chronic Eczema in Mice

Sai LIU; Na LANG; Jin-ning ZHAO; Xiao-di FAN; Xiao-xia DONG; Jian-hua FU

doi:10.13422/j.cnki.syfjx.20201708

您当前的位置：

首页 >

文章列表页 >

Effect of Euphorbiae Ebarcteolatae Radix Bacteriostasis Ointment on Chronic Eczema in Mice

Pharmacology | 更新时间：2020-09-09

- Effect of Euphorbiae Ebarcteolatae Radix Bacteriostasis Ointment on Chronic Eczema in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 17, Pages: 36-41(2020)
- 作者机构：
  
  中国中医科学院西苑医院，北京 100091
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201708
  CLC： R2-0;R22;R285.5;R289
- Published Online：28 June 2020，
  
  Published：05 September 2020
- 稿件说明：
移动端阅览
刘赛,郎娜,赵晋宁等.狼毒抑菌膏对小鼠慢性湿疹的干预作用[J].中国实验方剂学杂志,2020,26(17):36-41.

LIU Sai,LANG Na,ZHAO Jin-ning,et al.Effect of Euphorbiae Ebarcteolatae Radix Bacteriostasis Ointment on Chronic Eczema in Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):36-41.
刘赛,郎娜,赵晋宁等.狼毒抑菌膏对小鼠慢性湿疹的干预作用[J].中国实验方剂学杂志,2020,26(17):36-41. DOI： 10.13422/j.cnki.syfjx.20201708.

LIU Sai,LANG Na,ZHAO Jin-ning,et al.Effect of Euphorbiae Ebarcteolatae Radix Bacteriostasis Ointment on Chronic Eczema in Mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):36-41. DOI： 10.13422/j.cnki.syfjx.20201708.

摘要

目的

观察狼毒抑菌膏对2

4-二硝基氯苯(DNCB)诱导小鼠湿疹的影响。

方法

采用ICR小鼠40只，随机分为4组，每组10只，分别为正常组，模型组，尤卓尔组(0.09 g·kg

-1

)，狼毒抑菌膏组(0.09 g·kg

-1

)，除正常组外，其他各组小鼠耳廓及背部涂抹DNCB诱导建立慢性湿疹模型，24 h后经耳廓及背部给予相应药物，每日2次，连续10 d。实验结束后检测小鼠耳肿胀厚度与质量，进行耳变应评分，计算小鼠的脾脏指数，观察小鼠耳组织病理学变化，采用多因子检测技术测定白细胞介素-1

（IL-1

），人肿瘤坏死因子-

（TNF-

），白细胞介素-2（IL-2），白细胞介素-4（IL-4）等血清炎症因子的变化。

结果

与正常组比较，模型组小鼠右耳厚度及重量、耳肿胀度、耳廓变应评分、脾脏指数和血清中IL-2与IL-4水平明显升高（

0.05，

0.01），并出现明显的湿疹样病理改变。与模型组比较，狼毒抑菌膏可有效减轻模型组小鼠的耳廓肿胀程度、降低模型组耳廓变应评分和脾脏指数、同时明显降低血清IL-2与IL-4水平（

0.05，

0.01），改善耳部组织病理形态。

结论

狼毒抑菌膏通过一定程度的抗炎作用达到慢性湿疹的治疗效果。

Abstract

Objective

To observe the anti-inflammatory effect and its mechanism of Euphorbiae Ebarcteolatae Radix bacteriostasis ointment in eczema mice induced by 2

4-dinitrochlorobenzene (DNCB).

Method

A total of 40 ICR adult mice were randomly divided into control group，model group，hydrocortisone Butyrate cream group (0.09g·kg

-1

) and Euphorbiae Ebarcteolatae Radix bacteriostasis ointment group (0.09 g·kg

-1

)

with 10 mice in each group. Except for the normal group

other groups were given DNCB to induce the chronic eczema model. Twenty-four hours after DNCB stimulation

they were given the corresponding drugs through auricle and back

twice a day for 10 days. After drug intervention

efforts were made to measure the change of thickness and weight of the middle ear

assess the allergic effect

and calculate the spleen index of the mice. Optical microscope was used to observe the pathological changes in ear tissues of mice. And the levels of serum interleukin-1

(IL-1

)

tumor necrosis factor-

(TNF-

)

interleukin-2 (IL-2)

interleukin-4 (IL-4) in mice were determined by multiplex immunoassay.

Result

Compared with control group

the thickness and weight of right ears

score of allergic effect

spleen index and the levels of IL-2 and IL-4 in serum showed significant increases in model group (

0.05，

0.01). The histopathology injuries of ear were aggravated. Compared with model control group

Euphorbiae Ebarcteolatae Radix bacteriostasis ointment could reduce ear thickness and score of allergic effect

regulate the spleen index

decrease the inflammation factor in serum such as IL-2 and IL-4 (

0.05，

0.01)

and improve ear histopathology injuries.

Conclusion

Euphorbiae Ebarcteolatae Radix bacteriostasis ointment may have a good effect on eczema.

关键词

Keywords

references

王小勇 , 陶承军 , 应航宇 , 等 . 自制止痒湿疹合剂治疗慢性湿疹疗效及对血清IL-1、IL-2、IL-6 水平的影响 [J]. 中国中西医结合皮肤性病学杂志 , 2016 , 15 ( 4 ): 221 - 224 .

蒋祖玲 , 汤倩倩 , 黄安 , 等 . 慢性湿疹的药物外治治疗研究进展 [J]. 医学综述 , 2018 , 4 ( 20 ): 4103 - 4107 .

BROWN S J . Molecular mechanisms in atopic eczema:insights gained from genetic studies [J]. J Pathol , 2017 , 241 ( 2 ): 140 - 145 .

李晓瑞 , 朱丽红 , 李萍 . 婴儿湿疹相关因素的研究现状及进展 [J]. 新疆医学 , 2016 , 46 ( 12 ): 1565 - 1568 .

PARK S J , LEE Y H , LEE K H , et al . Effect of eriodictyol on the development of atopic dermatitis-like lesions in ICR mice [J]. Biol Pharm Bull , 2013 , 36 ( 8 ): 1375 - 1379 .

田硕 , 白明 , 苗明三 . 基于临床病症特点的湿疹动物模型分析 [J]. 中国中医杂志 , 2018 , 43 ( 4 ): 853 - 856 .

HON K L , LEONG K F , LEUNG T N H , et al . Dismissing the fallacies of childhood eczema management: case scenarios and an overview of best practices [J]. Drugs Context , 2018 , doi: 10.7573/dic.212547 http://dx.doi.org/10.7573/dic.212547 .

朱聪聪 , 潘会君 , 朱全刚 . Th2相关炎症因子在湿疹发病中的作用机制 [J]. 药学实践杂志 , 2019 , 37 ( 1 ): 9 - 13 .

万姜维 , 刘锋 . 中医药治疗糖皮质激素副作用研究进展 [J]. 河南中医 , 2016 , 36 ( 5 ): 914 - 917 .

夏青 , 徐柯心 , 张文婷 , 等 . 中药狼毒化学成分与药理作用概述 [J]. 环球中医药 , 2017 , 10 ( 8 ): 1027 - 1032 .

裴小平 , 杨斌 , 陈文静 , 等 . 白鲜皮提取液对湿疹模型小鼠血清IL-10、IL-17和IL-22含量的影响 [J]. 皮肤性病诊疗学杂志 , 2017 , 24 ( 3 ): 168 - 172 .

PENG M L , DAI S P , WANG S F , et al . Study on the anti-inflammatory effects of Dihu paste [J]. Chin J Hosp Pharm , 2012 , 2 ( 15 ): 1217 - 1219 .

范慧婕 , 王英燚 , 陈嘉蓥 , 等 . 海洋深层水对慢性湿疹小鼠的作用及其机制 [J]. 暨南大学学报 , 2017 , 38 ( 2 ): 114 - 120 .

FAN X , WANG J , HOU J , et al . Berberine alleviates ox-LDL induced inflammatory factors by up-regulation of autophagy via AMPK/mTOR signaling pathway [J]. J Transl Med , 2015 , doi: 10.1186/s12967-015-0450-z http://dx.doi.org/10.1186/s12967-015-0450-z .

朱卉雯 , 田静 , 李忻红 , 等 . 健脾养血祛风方对脾虚证慢性湿疹小鼠模型干预的实验研究 [J]. 现代中西医结合杂志 , 2016 , 25 ( 3 ): 235 - 238 .

XIANG N , LI X M , ZHANG M J , et al . Total glucosides of paeony can reduce the hepatotoxicity caused by Methotrexate and Leflunomide combination treatment of active rheumatoid arthritis [J]. Int Immunopharmacol , 2015 , 28 ( 1 ): 802 - 807 .

SONG H S , JUNG S E , KIM Y C , et al . Nipple eczema,an indicative manifestation of atopic dermatitis,a clinical,histological,and immunohistochemical study [J]. Am J Dermatopathol , 2015 , 37 ( 4 ): 284 - 288 .

李君蕊 , 赵宝春 , 辛桂荣 . 抗炎因子与感染的关系研究进展 [J]. 中国煤炭工业医学杂志 , 2008 , 11 ( 2 ): 268 - 270 .

SILVESTRE M C , REIS V M S D . Evaluation of the profile of inflammatory cytokines,through immunohistochemistry,in the skin of patients with allergic contact dermatitis to nickel in the acute and chronic phases [J]. An Bras Dermatol , 2018 , 93 ( 6 ): 829 - 835 .

刘欢 , 曹雯 , 韩清娟 , 等 . （ E ）-苯乙基-3-（3,5-二羟基-4-异丙基苯基）丙烯酸酯对小鼠皮炎湿疹的治疗作用初步研究 [J]. 免疫学杂志 , 2016 , 32 ( 4 ): 299 - 304 .

BERKER M , FRANK L J , GEBNER A L , et al . Allergies -A T cells perspective in the era beyond the TH1/TH2 paradigm [J]. Clin Immunol , 2017 , 174 : 73 - 83 .

张明发 , 沈雅琴 . 白鲜皮药理作用的研究进展 [J]. 抗感染药学 , 2012 , 9 ( 2 ): 95 - 98 .

闫梦茹 , 张文博 , 罗国平 , 等 . 地肤子抑菌活性成分筛选 [J]. 化学与生物工程 , 2019 , 36 ( 2 ): 28 - 31 .

蒋剑平 , 沈小青 , 范海珠 . 地肤子化学成分及药理活性研究进展 [J]. 中华中医药学刊 , 2011 , 29 ( 12 ): 2704 - 2706 .

贾丽娜 , 康学智 , 张栩 . 蛇床子素现代药理研究进展 [J]. 西北中医药 , 2018 , 31 ( 4 ): 141 - 145 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Compatibility of Ephedrae Herba，Asari Radix et Rhizoma， and Aconiti Lateralis Radix Praeparata on ILC2s-Related Factors in Lung of Allergic Rhinitis Model Mice

Therapeutic Effect of Bushen Huoxue Prescription on Diuretic Resistance in Chronic Heart Failure

Effect of Coptidis Rhizoma-Fermentum Rubrum on Non-Alcoholic Fatty Liver Disease in Mice Based on Nrf2/GPX4 Ferroptosis Axis

Repair Effect of Jiechang Buxu Pills on Mucosal Barrier Injury in Chronic Relapsing Ulcerative Colitis （Syndrome of Spleen-kidney Yang Deficiency）

Effect of Xiaoyaosan on Apoptosis in Mouse Model of Lipopolysaccharide-induced Depressive-like Behavior in Mice

Related Author

ZHANG Yi

TAO Xiaohua

LIU Min

SUN Yuchen

ZHANG Yan

YIN Wenhao

ZHAO Shujun

ZHANG Muchen

Related Institution

Beijing University of Chinese Medicine

The Affiliated Hospital of Liaoning University of TCM

Liaoning University of Traditional Chinese Medicine（TCM）

Zigong Hospital of TCM

School of Basic Medicine，Chengdu University of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰