Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on <italic style="font-style: italic">γ</italic>-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats

Bin GUO; Peng-han WANG; Lin-xing HUANG; Zhi-qiang XIE; Li-na XIE; Zeng-hui YUE

doi:10.13422/j.cnki.syfjx.20201739

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Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on γ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats

Pharmacology | 更新时间：2020-09-09

- Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on γ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 17, Pages: 29-35(2020)
- 作者机构：
  
  湖南中医药大学针灸推拿学院，长沙 410208
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201739
  CLC： R2-0;R289;R245;R322.81
- Published Online：01 July 2020，
  
  Published：05 September 2020
- 稿件说明：
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郭斌,王彭汉,黄麟荇等.加味芍药甘草汤、电针“曲池”“阳陵泉”穴位及针药结合方法对SCA大鼠皮质中γ-氨基丁酸能相关受体的影响[J].中国实验方剂学杂志,2020,26(17):29-35.

GUO Bin,WANG Peng-han,HUANG Lin-xing,et al.Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on γ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):29-35.
郭斌,王彭汉,黄麟荇等.加味芍药甘草汤、电针“曲池”“阳陵泉”穴位及针药结合方法对SCA大鼠皮质中γ-氨基丁酸能相关受体的影响[J].中国实验方剂学杂志,2020,26(17):29-35. DOI： 10.13422/j.cnki.syfjx.20201739.

GUO Bin,WANG Peng-han,HUANG Lin-xing,et al.Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on γ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(17):29-35. DOI： 10.13422/j.cnki.syfjx.20201739.

摘要

目的

分析脑卒中痉挛状态（SCA）大鼠肌张力损伤情况与大脑皮质区

-氨基丁酸

受体（GABA

），

-氨基丁酸转运体（GAT-1）蛋白及mRNA表达情况，探讨加味芍药甘草汤、电针曲池、阳陵泉穴位及针药结合3种方法缓解脑卒中肢体痉挛的疗效。

方法

通过2次随机分组，分为空白组、假手术组、模型组、加味芍药甘草汤组、针药结合组、电针组，每组9只。采用改良Zea-Longa线栓法+内囊注射NMDA受体法制备脑卒中肢体痉挛大鼠模型，行为学评分确认模型成功后，分别对电针组（电针双侧阳陵泉、曲池，30 min/次/d，持续5 d），加味芍药甘草汤组（加味芍药甘草汤水煎剂1次/d，每次灌胃10 mL·kg

-1

，持续5 d），针药结合组（加味芍药甘草汤灌服；再行电针治疗，持续5 d）进行干预。治疗后行为学检测的肌张力情况，采用实时荧光定量PCR（Real-time PCR）和蛋白免疫印迹法（Western blot）检测皮质中GABA

，GAT-1 mRNA与蛋白的表达。

结果

与正常组比较，模型组肌张力显著增高（

0.01），GABA

mRNA与蛋白的表达显著降低(

0.01)，GAT-1 mRNA与蛋白的表达显著上升(

0.01)；与模型组比较，各治疗组肌张力评分明显降低（

0.05），GABA

mRNA与蛋白的表达明显升高(

0.05，

0.01)，GAT-1 mRNA与蛋白的表达明显下降(

0.05，

0.01)，其中针药结合组相应表达最显著。

结论

加味芍药甘草汤、电针曲池、阳陵泉穴位及针药结合三种疗法均可以缓解脑卒中肢体痉挛的肌张力情况，而针药结合治疗方法对皮质中

-氨基丁酸能受体表达情况效能最高，提示其改善脑卒中肢体兴奋性痉挛状态的作用最优。

Abstract

Object

To analyze the effect of muscular tension injury in spasticity of cerebral apoplexy(SCA) rats with spasticity and the expression of

-aminobutyric acid receptor (GABA

)

-aminobutyric acid transporter (GAT-1) protein and mRNA expression in cerebral cortex modified Shaoyao Gancaotang

electroacupuncture Quchi

Yanglingquan and acupuncture combined with herb therapies alleviate the effect of SCA.

Method

Randomly divided into two groups

divided into blank group

sham operation group

model group

modified Shaoyao Gancaotang group

acupuncture combined with herb group

electroacupuncture group

selected qualified SD rats into the group

9 rats in each group. The modified Zea-Longa thread embolism method+internal capsule injection of NMDA receptor method was used to prepare a rat model. After the behavioral score confirms the success of the model

dispose of each group separately

electroacupuncture group (electroacupuncture on both sides of Yangling Spring and Quchi

1 time/d for 5 days)

modified Shaoyao Gancaotang group (with Shaoyao Gancaotang 1 time/d

10 mL·kg

-1

for 5 days)

acupuncture combined with herb (modified Shaoyao Gancaotang was given

then electroacupuncture treatment was continued for 5 days). After treatment

the muscle tension was detected by behavioral analysis. Real-time fluorescent quantitative PCR（Real-time PCR） and Western blot were used to detect the expression of GABA

and GAT-1 related mRNA and protein in the cortex.

Result

Compared with normal group

the muscle tension of the model group increased

0.01)

the expression of GABA

mRNA and protein decreased

0.01)

and the expression of GAT-1 mRNA and protein increased (

0.01)

compared with model group

the muscle tone score of the each treatment group decreased (

0.05)

the expression of GABA

mRNA and protein increased (

0.05，

0.01)

and the expression of GAT-1 mRNA and protein decreased (

0.05，

0.01)

of which the corresponding expression in the acupuncture combined with herb group was the most significant.

Conclusion

Electroacupuncture Quchi

Yanglingquan

modified Shaoyao Gancaotang and acupuncture combined with three therapies can alleviate the muscle tension of limbs spasm of stroke

and the combination of acupuncture and medicine treatment on the cortical

-aminobutyric acid receptor the highest expression efficiency suggests that it has the best effect on improving the excitatory spasticity of stroke limbs.

关键词

Keywords

references

姜梦石 , 包烨华 , 楚佳梅 , 等 . 头穴久留针结合分期电针对脑卒中患者步行能力的影响 [J]. 成都中医药大学学报 , 2019 ， 42 ( 1 ): 62 - 66 .

罗艳 . 针灸联合康复治疗脑卒中后运动性失语的临床效果及对NIHSS评分的影响观察 [J]. 当代医学 , 2018 ， 24 ( 34 ): 150 - 152 .

冯雅娟 , 贾晓沛 , 骆莉 , 等 . 蜡疗合通窍活血中药治疗脑卒中后痉挛性瘫痪疗效及对Gly、GABA水平的影响 [J]. 现代中西医结合杂志 , 2018 , 27 ( 7 ): 695 - 698 .

郭斌 , 岳增辉 , 谢志强 , 等 . 基于大脑黑质纹状体内多巴胺受体亚型含量与表达观察针刺对大鼠脑卒中痉挛状态的影响 [J]. 中华中医药杂志 , 2019 , 34 ( 2 ): 577 - 579 .

王生万 , 胡镜清 . 基于Glu/GABA分子系统的阴阳属性探讨 [J]. 中国中医基础医学杂志 , 2018 , 24 ( 3 ): 289 - 293 .

张飞燕 , 李晶晶 , 周莹 , 等 . 安神类中药及其有效成分对神经递质镇静催眠机制的研究进展 [J]. 中国中药杂志 , 2016 , 41 ( 23 ): 4320 - 4327 .

田书林 , 冯卫红 , 唐民科 . 成年哺乳动物神经发生的关键调控环节:GABA的作用由兴奋到抑制的转变 [J]. 生理科学进展 , 2009 , 40 ( 3 ): 257 - 260 .

LIN Y , BREUGELMANS J , IVERSEN M , et al . An adaptive interface de-sign (AID) for enhanced computer accessibility and rehabilitation [J]. Int J Hum-Comput St , 2017 , doi： 10.1016/j.ijhcs.2016.09.012 http://dx.doi.org/10.1016/j.ijhcs.2016.09.012 .

MOORE M , JIMENEZ N , ROWHANIRAHBAR A , et al . Availability of outpa-tient rehabilitation services and barriers to care for vulnerablepopulations after pediatric traumatic brain injury [J]. Am J Phys Med Rehabil , 2016 , 95 ( 3 ): 204 .

王景霞 , 杨旭 , 张建军 , 等 . 芍药甘草汤对中枢性肌张力增高大鼠脑内氨基酸及其受体的影响 [J]. 中国中药杂志 , 2016 , 41 ( 6 ): 1100 - 1106 .

黄勇 , 杨晓姣 , 申震 , 等 . 芍药甘草汤对脊髓抑制性神经递质及大鼠脊髓损伤后肌痉挛的影响 [J]. 中华中医药杂志 , 2018 , 33 ( 9 ): 4200 - 4203 .

陈岚榕 , 刘呈艳 , 王林林 , 等 . 针药结合治疗脑卒中后吞咽障碍并发肺炎的疗效观察 [J]. 中国中医基础医学杂志 , 2019 , 25 ( 11 ): 1569 - 1571 .

唐卫华 , 张蓓蓓 , 丁砚兵 . 针药“病证结合”早期治疗急性缺血性中风的疗效观察 [J]. 中西医结合心脑血管病杂志 , 2018 , 16 ( 18 ): 2733 - 2735 .

刘未艾 , 岳增辉 , 付磊 , 等 . 经筋刺法结合加味芍药甘草汤治疗脑卒中后肢体痉挛的效果 [J]. 中国康复理论与实践 , 2018 , 24 ( 3 ): 323 - 327 .

孙培养 , 储浩然 , 李佩芳 , 等 . 通督调神针刺干预对药物治疗脑卒中后抑郁的影响 [J]. 中国针灸 , 2015 , 35 ( 8 ): 753 - 757 .

岳增辉 , 谢志强 , 谢莉娜 , 等 . 脑卒中后肢体痉挛大鼠模型制备方法 [J]. 中国中医药现代远程教育杂志 , 2019 , 17 ( 5 ): 129 - 131 .

BOHANNON R W , SMITH M B . Interrater reliability of a modi-fied Ashworth scale of muscle spasticity [J]. Phys Ther , 1987 , 67 ( 2 ): 206 - 207 .

刘鸿燕 , 周奇志 , 蔡定均 , 等 . 构建大鼠内囊电毁损痉挛模型 [J]. 中国老年学杂志 , 2008 , 28 ( 22 ): 2214 - 2215 .

郭义 . 实验针灸学实验指导 [M]. 北京：中国中医药出版社， 2012 : 47 - 48 .

王耀帅 , 张建斌 , 王玲玲 , 等 . 对针药结合研究现状与发展趋势的思考 [J]. 中医杂志 , 2012 , 53 ( 6 ): 473 - 475 .

王伟明 , 王星博 , 赵杰 . 针药并用在早发性卵巢功能不全防治中的价值初探 [J]. 针灸临床杂志 , 2020 ( 2 ): 84 - 88 .

钱颖 , 邵钰柔 , 卢圣锋 , 等 . 中医药治疗阿尔茨海默症的研究进展 [J]. 南京中医药大学学报 , 2019 , 35 ( 6 ): 761 - 766 .

李跃兵 . 向贤德教授针药结合治疗功能性消化不良临床经验 [J]. 中国针灸 , 2019 , 39 ( 10 ): 1089 - 1091 .

王莹 , 吴红杰 , 管庆霞 , 等 . 基于灰色关联分析法探索芍药甘草汤对癫痫小鼠脑保护作用的谱效关系 [J]. 中国实验方剂学杂志， 2020 ， doi： 10.13422/j.cnki.syfjx.20201160 http://dx.doi.org/10.13422/j.cnki.syfjx.20201160 .

黄勇 , 杨晓姣 , 申震 , 等 . 芍药甘草汤对脊髓抑制性神经递质及大鼠脊髓损伤后肌痉挛的影响 [J]. 中华中医药杂志 , 2018 , 33 ( 9 ): 4200 - 4203 .

周星娅 , 刘维红 , 刘涛 , 等 . 针灸治疗脑梗死后痉挛性瘫痪临床选穴规律研究 [J]. 中国中医基础医学杂志 , 2018 , 24 ( 7 ): 974 - 977 .

王东雁 , 吴河山 , 王建宇 , 等 . 电针配合穴位注射治疗脑出血后肩-手综合征临床观察 [J]. 新中医 , 2018 , 50 ( 7 ): 182 - 185 .

郭斌 , 岳增辉 , 谢志强 , 等 . 电针对脑卒中痉挛状态大鼠大脑皮质中谷氨酸与 γ -氨基丁酸二者受体表达的影响 [J]. 中华中医药杂志 , 2019 , 34 ( 1 ): 325 - 327 .

郭晓 , 郭蓉娟 , 邢佳 , 等 . 宁心安神方调控失眠大鼠Glu/GABA-Gln代谢环路失衡的机制研究 [J]. 北京中医药大学学报 , 2017 , 40 ( 5 ): 413 - 419 .

LI L ， ZHAO L ， WANG Y ， et al . PKCɛ mediates substance P inhibition of GABAAreceptors-mediated current in rat dorsal root ganglion [J]. J Huazhong Univer Sci Technol , 2015 , 35 ( 1 ): 1 - 9 .

DE L E , RAVASENGA T , PETRINI E M , et al . Inter-synaptic lateral diffusion of gaba α receptors shapes inhibi-tory synaptic currents [J]. Neuron ， 2017 ， 95 : 63 - 69 .

张舜波 , 王平 , 田代志 , 等 . 酸枣仁总皂苷对失眠老年大鼠脑氨基酸类神经递质及受体表达的影响 [J]. 中国实验方剂学杂志 , 2014 , 20 ( 4 ): 124 - 127 .

龙乾发 , 罗强 , 田晔 , 等 . 骨髓间充质干细胞对大鼠血管性痴呆模型GAT1的调节作用研究 [J]. 中华细胞与干细胞杂志：电子版 , 2017 , 7 ( 4 ): 208 - 213 .

张媛媛 , 王杰琼 , 王美艳 , 等 . 舒郁胶囊对经前期综合征肝气郁证大鼠顶区皮层GABA-T、GAT-1表达的影响 [J]. 中医杂志 , 2016 , 57 ( 13 ): 1137 - 1141 .

SHI J , CAI Y , LIU G , et al . Enhanced learning and memory in GAT1 heterozygous mice [J]. Acta Bioch Biophy Sin , 2012 , 44 ( 4 ): 359 - 366 .

MAKI B A , COLE R . Two serine residues on GluN2A C-terminal tailscontrol NMDA receptor current decay times [J]. Channels ， 2013 , 7 ( 2 ): 126 - 132 .

LU W , FANG W , LI J , et al . Phosphorylation of tyrosine 1070 at the GluN2B subunit is regulated by synaptic activity and critical for surface expression of N-methyl-D-aspartate (NMDA) receptors [J]. J Biol Chem , 2015 , 290 ( 38 ): 22945 - 22954 ．

黄喜 , 陈慧英 , 韦廷佳 , 等 . NMDA受体靶向拮抗剂的研究进展 [J]. 生理科学进展 , 2018 , 49 ( 3 ): 212 - 216 .

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⁰

Effect of Modified Shaoyao Gancaotang， Electroacupuncture and Acupuncture Combined with Herb on γ-Aminobutyric Acid Related Receptor Proteins in Cortex in SCA Rats

DOI：10.13422/j.cnki.syfjx.20201739

摘要

Abstract

关键词

Keywords

references