Protective Effect of Modified Sinisan on Depression-induced Liver Injury in Rat

Xin CHEN; Xiong-jun MOU; Hao LIU; Yan-wen LIU; Song-lin LIU

doi:10.13422/j.cnki.syfjx.20201822

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Protective Effect of Modified Sinisan on Depression-induced Liver Injury in Rat

Classic Prescriptions | 更新时间：2020-09-09

- Protective Effect of Modified Sinisan on Depression-induced Liver Injury in Rat
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 18, Pages: 18-23(2020)
- 作者机构：
  
  湖北中医药大学，中药资源与中药化学湖北省重点实验室，武汉 430065
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201822
  CLC： R22;R242;R284.1;R285.5
- Received：23 January 2020，
  
  Published Online：16 July 2020，
  
  Published：20 September 2020
- 稿件说明：
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陈新,牟雄军,刘昊等.加味四逆散对抑郁模型大鼠肝损伤的保护作用[J].中国实验方剂学杂志,2020,26(18):18-23.

CHEN Xin,MOU Xiong-jun,LIU Hao,et al.Protective Effect of Modified Sinisan on Depression-induced Liver Injury in Rat[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):18-23.
陈新,牟雄军,刘昊等.加味四逆散对抑郁模型大鼠肝损伤的保护作用[J].中国实验方剂学杂志,2020,26(18):18-23. DOI： 10.13422/j.cnki.syfjx.20201822.

CHEN Xin,MOU Xiong-jun,LIU Hao,et al.Protective Effect of Modified Sinisan on Depression-induced Liver Injury in Rat[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):18-23. DOI： 10.13422/j.cnki.syfjx.20201822.

摘要

目的

研究加味四逆散对慢性温和不可预知性应激（CUMS）结合孤养法制作抑郁模型所致肝损伤的保护作用。

方法

将48只SD雄性大鼠随机分为6组，正常组，抑郁模型组，氟西汀（1.58 mg·kg

-1

·d

-1

）组，和加味四逆散低、中、高剂量（3.67，7.34，14.68 g·kg

-1

·d

-1

）组，每组8只。造模给药结束后，采用生化试剂盒法检测各组大鼠血清中丙氨酸氨基转移酶（ALT），天冬氨酸氨基转移酶（AST）水平和肝脏组织中炎症因子肿瘤坏死因子-

（TNF-

），白细胞介素-1

（IL-1

），白细胞介素-6（IL-6）水平。采用蛋白免疫印迹法（Western blot）检测加味四逆散对肝细胞中半胱氨酸蛋白酶-3（Caspase-3），活化型半胱氨酸蛋白酶-3（cleaved Caspase-3）蛋白表达的干预作用。

结果

生化试剂盒检测显示，与正常组比较，模型组大鼠血清中的ALT，AST水平明显升高（

0.05），肝脏组织中炎症因子TNF-

，IL-1

和IL-6水平明显升高（

0.05）；与模型组比较，加味四逆散低、中剂量组可降低血清中ALT，AST和炎症因子TNF-

，IL-1

和IL-6水平（

0.05，

0.01）。Western blot检测显示，与正常组比较，模型大鼠肝细胞中的Caspase-3蛋白表达下降，cleaved Caspase-3蛋白表达上升（

0.05）；与模型组比较，加味四逆散低、中剂量组可以上调Caspase-3蛋白的表达，下调cleaved Caspase-3蛋白的表达（

0.05，

0.01）。

结论

慢性应激反应所致抑郁模型可造成肝损伤，加味四逆散能够通过调节多种生理生化和炎症因子指标的水平，抑制肝脏细胞凋亡来达到保肝作用。

Abstract

Objective

To study the protective effect of modified Sinisan on liver injury caused by chronic unpredictable mild stress (CUMS) combined with solitary nutrition method based on a depression model.

Method

Forty-eight SD male rats were randomly divided into normal group

depression model group

fluoxetine group (1.58 mg·kg

-1

·d

-1

)

and low

medium and high-dose modified Sinisan (3.67

7.34

14.68 g·kg

-1

·d

-1

) groups

with 8 rats in each group. After the administration of the model

the levels of alanine aminotransferase（ALT）

aspartate aminotransferase（AST） in serum and the levels of inflammatory factors tumor necrosis factor-

（TNF-

）

interleukin（IL）-1

and IL-6 in liver tissues were measured by a biochemical kit method. Western blot was used to detect the expressions of cysteine aspastic acid-specific protease-3 (Caspase-3) and cleaved Caspase-3 in liver cells after intervention with modified Sinisan.

Result

Compared with the normal group

the biochemical test showed that the levels of ALT and AST in the serum of the model group were significantly increased (

0.05)

and the levels of inflammatory factors TNF-

IL-1

and IL-6 in liver tissue significantly increased (

0.05). Compared with the model group

low

medium-dose modified Sinisan groups significantly reduced serum ALT

AST and inflammatory factors TNF-

IL-1

and IL-6 levels (

0.05). Western blot showed that compared with the normal group

the protein expression of Caspase-3 in model rat liver cells decreased

while the protein expression of cleaved Caspase-3 increased（

0.05，

0.01）. Compared with the model group

low

medium-dose modified Sinisan groups could up-regulate Caspase-3 protein expression

and down-regulated the expression of cleaved Caspase-3 protein（

0.05，

0.01）.

Conclusion

Chronic stress-induced depression model can cause liver damage. Modified Sinisan can have the hepatoprotective effect by regulating the levels of various physiological

biochemical and inflammatory factor indicators

and inhibiting liver cell apoptosis.

关键词

Keywords

references

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