Protective Effect of Cangxi Tongbi Capsule on Articular Cartilage of KOA Rats Based onp38 MAPK Signaling Pathway

Wen-peng XIE; Ling-sen YANG; Liang MA; Xiang-peng WANG; Xu-yu SONG; Rong-xiu BI

doi:10.13422/j.cnki.syfjx.20201837

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Protective Effect of Cangxi Tongbi Capsule on Articular Cartilage of KOA Rats Based onp38 MAPK Signaling Pathway

Pharmacology | 更新时间：2020-09-09

- Protective Effect of Cangxi Tongbi Capsule on Articular Cartilage of KOA Rats Based onp38 MAPK Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 18, Pages: 70-76(2020)
- 作者机构：
  
  1.山东中医药大学附属医院，济南 250355
  2.山东中医药大学，济南 250014
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201837
  CLC： R2-0;R289;R285.5;R274.9
- Received：09 January 2020，
  
  Published Online：13 July 2020，
  
  Published：20 September 2020
- 稿件说明：
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谢文鹏,杨灵森,马亮等.基于p38 MAPK信号通路探讨苍膝通痹胶囊对KOA大鼠关节软骨的保护作用[J].中国实验方剂学杂志,2020,26(18):70-76.

XIE Wen-peng,YANG Ling-sen,MA Liang,et al.Protective Effect of Cangxi Tongbi Capsule on Articular Cartilage of KOA Rats Based onp38 MAPK Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):70-76.
谢文鹏,杨灵森,马亮等.基于p38 MAPK信号通路探讨苍膝通痹胶囊对KOA大鼠关节软骨的保护作用[J].中国实验方剂学杂志,2020,26(18):70-76. DOI： 10.13422/j.cnki.syfjx.20201837.

XIE Wen-peng,YANG Ling-sen,MA Liang,et al.Protective Effect of Cangxi Tongbi Capsule on Articular Cartilage of KOA Rats Based onp38 MAPK Signaling Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(18):70-76. DOI： 10.13422/j.cnki.syfjx.20201837.

摘要

目的

观察苍膝通痹胶囊治疗膝骨关节炎（KOA）模型大鼠的疗效及相关作用机制。

方法

将60只4周龄SPF级健康雄性SD大鼠，随机分为空白组，模型组，二甲基亚砜（DMSO）组，苍膝通痹胶囊组，SB203580组以及苍膝通痹胶囊联合SB203580组，每组10只。除正常组外，其余各组采用改良Hulth法建立KOA模型，造模成功后，苍膝通痹胶囊组每日给予0.25 g·kg

-1

苍膝通痹胶囊溶液灌胃，SB203580组每日给予0.015 g·kg

-1

的SB203580溶液灌胃，苍膝通痹胶囊联合SB203580组组每日给予含0.015 g·kg

-1

SB203580及0.25 g·kg

-1

苍膝通痹胶囊的混合溶液灌胃，DMSO组给予1% DMSO溶液灌胃，模型组和空白组给予生理盐水灌胃，用药干预4周后处死、取材。苏木素-伊红(HE)染色观察软骨组织形态学改变，酶联免疫吸附测定（ELISA）检测外周血上清液中白细胞介素-1

（IL-1

），肿瘤坏死因子-

（TNF-

）表达水平，实时荧光定量PCR（Real-tine PCR）及蛋白免疫印迹法（Western blot）检测软骨组织中p38 MAPK信号通路中相关因子p38，p-p38，基质金属蛋白酶-13（MMP-13），Ⅱ型胶原蛋白（CollagenⅡ）mRNA及蛋白的表达水平，免疫组化法检测p-p38的定位表达。

结果

与正常组比较，模型组关节软骨中p38，p-p38，MMP-13表达水平显著上调（

0.01），CollagenⅡ表达水平显著下调（

0.01），血清IL-1

，TNF-

表达水平显著上调（

0.01）；与模型组比较，苍膝通痹胶囊组，SB203580组以及苍膝通痹胶囊联合SB203580组关节软骨中p38，p-p38，MMP-13表达水平显著下调（

0.01），CollagenⅡ表达水平显著上调（

0.01），血清IL-1

，TNF-

表达水平显著下调（

0.01）。

结论

苍膝通痹胶囊可有效保护KOA大鼠软骨组织，其机制可能与靶向阻断p38 MAPK信号通路有关。

Abstract

Objective

To observe the therapeutic effect and mechanism of Cangxi Tongbi capsule on knee osteoarthritis (KOA) model rats.

Method

Sixty 4-week-old SPF healthy male SD rats were randomly divided into blank group

model group

dimethyl sulfoxide (DMSO) group

Cangxi Tongbi capsule group

SB203580 group and Cangxi Tongbi capsule combined with SB203580 group. In addition to the normal group

the modified Hulth method was used to establish the koa model. After the model was established successfully

the Cangxi Tongbi capsule group was given 0.25 g·kg

-1

Cangxi Tongbi capsule solution by gavage every day

the SB203580 group was given 0.015 g·kg

-1

SB203580 solution by gavage

the Cangxi Tongbi capsule combined with SB203580 group was given a mixed solution containing 0.015 g·kg

-1

SB203580 and 0.25 g·kg

-1

Cangxi Tongbi capsule by gavage

the DMSO group was given 1% DMSO solution by gavage

the model group and blank group were given normal saline by gavage The stomach was killed and the material was taken after 4 weeks of drug intervention. The expression levels of interleukin-1

（IL-1

） and tumor necrosis factor-

（TNF-

）in the supernatant of peripheral blood were detected by ELISA. p38

p-p38

matrix metalloproteinase-13(MMP-13)

Collagen Ⅱ mRNA and protein in p38 MAPK signal pathway were detected by Real-time fluorescence quantification PCR （Real-time PCR） and Western blot

and the localization expression of p-p38 was detected by immunohistochemistry.

Result

Compared with normal group，the expression levels of p38，p-p38，MMP-13 in articular cartilage of the model group were up-regulated (

0.01)

the expression levels of CollagenⅡ was down-regulated (

0.01). The contents of IL-1

and TNF-

in serum were significantly increased (

0.01). Compared with model group

the expression levels of p38，p-p38，MMP-13 in articular cartilage of the Cangxi Tongbi capsule group

SB203580 group and Cangxi Tongbi capsule combined with SB203580 group were down-regulated (

0.01)

the expression levels of CollagenⅡ was up-regulated (

0.01). The contents of IL-1

and TNF-

in serum were significantly decreased (

0.01).

Conclusion

Cangxi Tongbi capsule can effectively protect the cartilage of KOA rats

and its mechanism may be related to the targeted blocking of p38 MAPK signal pathway.

关键词

Keywords

references

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