Effect of Plantaginis Semen<italic style="font-style: italic"> </italic>on Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats

Yue WANG; Ya-ning BIAO; Xin-qiao CHU; Na-bo ZHANG; Mu-qing ZHANG; Xi WANG; Xue HAN; Yi-xin ZHANG

doi:10.13422/j.cnki.syfjx.20201902

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Effect of Plantaginis Semen on Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats

Pharmacology | 更新时间：2020-09-16

- Effect of Plantaginis Semen on Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 52-58(2020)
- 作者机构：
  
  1.河北中医学院药学院，河北省高校中药组方制剂应用技术研究中心，石家庄 050200
  2.河北省中医院，石家庄 050011
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201902
  CLC： R2-0;R22;R285.5
- Received：05 February 2020，
  
  Published Online：08 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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王月,彪雅宁,储心乔等.车前子对腹泻大鼠炎性因子和结肠组织AQP4 mRNA和蛋白表达的影响[J].中国实验方剂学杂志,2020,26(20):52-58.

WANG Yue,BIAO Ya-ning,CHU Xin-qiao,et al.Effect of Plantaginis Semenon Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):52-58.
王月,彪雅宁,储心乔等.车前子对腹泻大鼠炎性因子和结肠组织AQP4 mRNA和蛋白表达的影响[J].中国实验方剂学杂志,2020,26(20):52-58. DOI： 10.13422/j.cnki.syfjx.20201902.

WANG Yue,BIAO Ya-ning,CHU Xin-qiao,et al.Effect of Plantaginis Semenon Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):52-58. DOI： 10.13422/j.cnki.syfjx.20201902.

摘要

目的

观察车前子对腹泻大鼠血清中炎性因子和结肠组织水通道蛋白4（AQP4） mRNA和蛋白表达的影响，探讨其治疗腹泻的作用机制。

方法

采用番泻叶药液灌胃法复制大鼠腹泻模型。将60只SD雄性大鼠随机分为正常组、模型组、氢氯噻嗪组（9 mg·kg

-1

）和车前子低、中、高剂量组（0.95，1.9，3.8 g·kg

-1

）。除正常组外，其余各组均于每日上午灌胃番泻叶药液（20 mL·kg

-1

），正常组灌胃等量的蒸馏水；每日下午各治疗组灌服相应药物，正常组和模型组灌服等量蒸馏水，连续14 d。实验结束后，根据粪便性状和大便次数，统计对比各组大鼠的稀便率、平均稀便级和腹泻指数，采集血清和结肠组织标本，检测血清中肿瘤坏死因子-

（TNF-

），白细胞介素-6（IL-6）和C反应蛋白（CRP）的含量；苏木素-伊红（HE）染色观察结肠黏膜病理形态学变化；实时荧光定量聚合酶链式反应（Real-time PCR）和蛋白免疫印迹法（Western blot）检测结肠组织中AQP4 mRNA和蛋白的表达情况。

结果

与正常组比较，模型组稀便率、平均稀便级和腹泻指数显著升高（

0.01），结肠黏膜表皮细胞出现凋亡坏死，固有层毛细血管扩张充血明显，少量中性粒细胞浸润，血清中TNF-

，IL-6和CRP的含量明显升高（

0.05，

0.01），AQP4 mRNA和蛋白的表达均明显下降（

0.01）；与模型组比较，车前子各剂量组大鼠的稀便率、平均稀便级和腹泻指数均显著降低（

0.01），结肠黏膜表皮细胞凋亡坏死、毛细血管扩张充血和中性粒细胞浸润现象明显改善，血清中TNF-

和CRP的含量降低（

0.05，

0.01），AQP4 mRNA和蛋白的表达水平明显升高（

0.05，

0.01）。

结论

车前子具有较好的止泻作用，其机制可能是抑制炎症反应、修复结肠黏膜病理损伤，上调AQP4的表达，调节水液代谢。

Abstract

Objective

To observe

Plantaginis Semen's mechanism in treating diarrhea by observing the effect on inflammatory factors in serum and mRNA and protein expressions of aquaporin4 (AQP4) in colon tissue of diarrhea rats.

Method

Senne Folium was orally administered to duplicate diarrhea rats. Sixty male SD rats were randomly divided into normal group

model group

hydrochlorothiazide group (9 mg·kg

-1

)

and low

middle

and high-dose Plantaginis Semen groups (0.95

1.9

3.8 g·kg

-1

). Senne Folium

(20 mL·kg

-1

) was intragastrically administered in 5 groups in the morning

except for normal group that was orally given the same dose of distilled water. In the afternoon

each treatment group was orally given the corresponding drugs

while normal group and model group were orally given the same dose of distilled water. The loose stool rate

average degree of loose stool

and diarrhea index were compared according to fecal traits and stool times after 14 days of treatment. The serum and colon tissue were collected to detect the contents of tumor necrosis factor-

(TNF-

)

interleukin-6 (IL-6) and C-reactive protein (CRP) in serum. Hematoxylin-eosin (HE) staining was used to observe the pathological morphological changes of colon tissue

and quantiative Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to detect the mRNA and protein expressions of AQP4 in colon tissue.

Result

In the model group

the loose stool rate

average degree of loose stool

and diarrhea index were significantly increased (

0.01)

apoptosis and necrosis were observed in the epidermal cells of colonic mucosa

telangiectasia and congestion in lamina propria were obvious

and a few neutrophils were infiltrated

and the contents of TNF-

IL-6 and CRP in serum increased (

0.05

0.01)

the mRNA and protein expressions of AQP4 significantly decreased (

0.01). Compared with the model group

the loose stool rate

average degree of loose stool

and diarrhea index were significantly decreased in low

middle

and high-dose Plantaginis Semen groups (

0.01)

the apoptosis and necrosis of epidermal cells

telangiectasia and hyperemia and neutrophil infiltration in colonic mucosa were obviously improved

and the contents of TNF-

and CRP in serum significantly decreased (

0.05

0.01)

the mRNA and protein expressions of AQP4 increased （

0.05

0.01）.

Conclusion

Plantaginis Semen has a better antidiarrheal effect

and its mechanism may be related to inhibition of inflammatory reaction

repair of pathological damage of colonic mucosa

up-regulation of AQP4 expression and promotion of water and fluid metabolism.

关键词

Keywords

references

石芳 , 郝旭曼 , 刘阳 , 等 . 胃肠安丸治疗功能性腹泻临床疗效评价 [J]. 中医临床研究 , 2018 , 10 ( 33 ): 15 - 18 .

谢明 , 杨爽爽 , 王亮亮 , 等 . 中药车前子的研究进展 [J]. 黑龙江医药 , 2015 , 28 ( 3 ): 474 - 476 .

王大勇 . 单味车前子治疗慢性腹泻2例 [J]. 中国社区医师:医学专业 , 2012 , 14 ( 8 ): 275 .

刘祺 . 复方车前健脾利湿止泻散联合常规用药治疗小儿秋季腹泻临床研究 [J]. 新中医 , 2019 , 51 ( 9 ): 171 - 173 .

张鲜桃 . 车前子汤治疗小儿腹泻 [J]. 陕西中医学院学报 , 1993 , 16 ( 3 ): 8 .

张桂花 , 孙焕明 , 段玉环 . 山药车前子汤治疗泄泻113例疗效观察 [J]. 河南中医药学刊 , 1994 , 9 ( 1 ): 51 - 52 .

陈文锋 , 宋丽军 , 赵文昌 . 水通道蛋白在腹泻中的研究进展 [J]. 医学综述 , 2018 , 24 ( 6 ): 1041 - 1046 .

SUNDELL K S , SUNDH H . Intestinal fluid absorption in anadromous salmonids:importance of tight junctions and aquaporins [J]. Front Physiol , 2012 , doi: 10.3389/fphys.2012.00388 http://dx.doi.org/10.3389/fphys.2012.00388 .

赵凯科 , 郑立 , 毛智斌 , 等 . 水通道蛋白与胃肠道疾病关系研究进展 [J]. 动物医学进展 , 2016 , 37 ( 1 ): 77 - 80 .

武志娟 , 张志敏 , 张大鹏 , 等 . 附子理中汤对腹泻型肠易激大鼠模型结肠组织中AQP8 mRNA表达水平的影响 [J]. 中药材 , 2014 , 37 ( 6 ): 1046 - 1048 .

谢婧 , 周祎青 , 郑锋玲 , 等 . 温病湿热证动物模型中炎症因子与水通道蛋白表达的实验研究 [J]. 中华中医药学刊 , 2018 , 36 ( 9 ): 2163 - 2166 .

张晶 , 陈志斌 , 王春娥 , 等 . 基于气道炎症调控的全真一气汤干预肾气虚型哮喘大鼠的机制研究 [J]. 中国中医急症 , 2019 , 28 ( 8 ): 1354 - 1357 .

甘江华 , 黄玙璠 , 彭代银 , 等 . 3种石斛对脾阴虚型大鼠便秘的治疗作用及其机制初步研究 [J]. 中国中药杂志 , 2019 , 44 ( 12 ): 2600 - 2606 .

刘洋 , 向丽华 , 孙刚 , 等 . 五苓散对腹泻模型大鼠结肠AQP4及AQP4mRNA表达的影响 [J]. 中国中医基础医学杂志 , 2012 , 18 ( 5 ): 547 - 549 .

郭艳丽 . 动物腹泻模型构建及进展 [J]. 世界医学最新信息文摘 , 2015 , 15 ( 44 ): 38 .

王珊娜 . 四神汤加减治疗脾虚泄泻的疗效观察 [J]. 中国疗养医学 , 2019 , 28 ( 7 ): 769 - 770 .

李志轩 , 赵云 , 王汉岑 . 利小便实大便治飧泄、洞泄、溏泄探讨 [J]. 新中医 , 2015 , 47 ( 4 ): 330 - 331 .

秦永照 . 利福昔明治疗急性细菌感染性腹泻疗效的调查分析 [J]. 浙江临床医学 , 2015 , 1 ( 7 ): 1139 - 1140 .

张根民 . 神曲消食口服液治疗小儿腹泻的疗效及其对IL-6、TNF- α 水平的影响 [J]. 北方药学 , 2019 , 16 ( 3 ): 142 - 143 .

RETTIG T C , VERWIJMEREN L , DIJKSTRA I M , et al . Postoperative interleukin6 level and early detection of complications after elective major abdominal surgery [J]. Ann Surg , 2016 , 263 ( 6 ): 1207 - 1212 .

王东悦 . 双歧杆菌三联活菌散对感染性腹泻患者IL-6、TNF- α 和CRP水平的影响 [J]. 中国实用医药 , 2019 , 14 ( 32 ): 111 - 113 .

杜丽军 , 雒军 , 吴国泰 , 等 . 当归对血虚便秘模型小鼠结肠水通道蛋白4表达的影响 [J]. 中药药理与临床 , 2017 , 33 ( 5 ): 103 - 107 .

王杨 , 彪雅宁 , 张纳博 , 等 . 车前子对腹泻大鼠小肠黏膜AQP1表达的影响 [J]. 河北中医药学报 , 2020 , 35 ( 2 ): 5 - 8 .

李立胜 , 王俊平 . 洛哌丁胺对腹泻模型大鼠结肠水通道蛋白4表达的影响 [J]. 胃肠病学和肝病学杂志 , 2013 , 22 ( 12 ): 1225 - 1227 .

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⁰

Effect of Plantaginis Semen on Inflammatory Cytokines and mRNA and Protein Expressions of AQP4 in Colon Tissue of Diarrhea Rats

DOI：10.13422/j.cnki.syfjx.20201902

摘要

Abstract

关键词

Keywords

references