Regulatory Effect of Modified Erchentang on Levels of Lymphokines Relating to Th1,Th2 and Th9 Cells and Expressions of IL-4R1/IL-13RA1 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease

Meng-di MAO; Li-zhi SHANG; Li-li XU; Shu JI; Long-tao SHI; Yao-yang LI; Guang-yuan ZHANG; Guo-qiang WANG; Wen-ying XIE

doi:10.13422/j.cnki.syfjx.20201905

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Regulatory Effect of Modified Erchentang on Levels of Lymphokines Relating to Th1,Th2 and Th9 Cells and Expressions of IL-4R1/IL-13RA1 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease

Classic Prescriptions | 更新时间：2020-09-16

- Regulatory Effect of Modified Erchentang on Levels of Lymphokines Relating to Th1,Th2 and Th9 Cells and Expressions of IL-4R1/IL-13RA1 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 16-24(2020)
- 作者机构：
  
  1.河南中医药大学，郑州 450046
  2.河南中医药大学第一临床医学院，郑州 450006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201905
  CLC： R2-0;R22;R285.5;R289
- Received：11 January 2020，
  
  Published Online：22 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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毛梦迪,尚立芝,徐莉莉等.二陈汤加味对慢阻肺大鼠Th1/2/9细胞相关淋巴因子及IL-4R1/IL-13RA1的调节作用[J].中国实验方剂学杂志,2020,26(20):16-24.

MAO Meng-di,SHANG Li-zhi,XU Li-li,et al.Regulatory Effect of Modified Erchentang on Levels of Lymphokines Relating to Th1,Th2 and Th9 Cells and Expressions of IL-4R1/IL-13RA1 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):16-24.
毛梦迪,尚立芝,徐莉莉等.二陈汤加味对慢阻肺大鼠Th1/2/9细胞相关淋巴因子及IL-4R1/IL-13RA1的调节作用[J].中国实验方剂学杂志,2020,26(20):16-24. DOI： 10.13422/j.cnki.syfjx.20201905.

MAO Meng-di,SHANG Li-zhi,XU Li-li,et al.Regulatory Effect of Modified Erchentang on Levels of Lymphokines Relating to Th1,Th2 and Th9 Cells and Expressions of IL-4R1/IL-13RA1 in Bronchioles of Rats with Chronic Obstructive Pulmonary Disease[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):16-24. DOI： 10.13422/j.cnki.syfjx.20201905.

摘要

目的

探究二陈汤加味对慢性阻塞性肺病（COPD）大鼠血浆、支气管肺泡灌洗液（BALF）中白细胞介素-12（IL-12），

-干扰素（IFN-

），IL-9，IL-4和IL-13水平以及对细支气管组织中IL-4受体1（IL-4R1），IL-13受体A1（IL-13RA1）定位表达的影响。

方法

将大鼠随机分为正常组，模型组，二陈汤加味低、中、高剂量组，共5组，每组10只。采用香烟烟雾联合脂多糖（LPS）方法制备COPD大鼠模型，二陈汤加味低、中、高剂量组分别以5，10，20 g·kg

-1

灌胃（

），正常组与模型组

等量生理盐水，连续干预14 d。采用酶联免疫吸附测定(ELISA)检测大鼠血浆，BALF中IL-12，IFN-

，IL-9，IL-4和IL-13水平，免疫组化（IHC）检测大鼠细支气管组织中IL-4R1，IL-13RA1的定位表达。

结果

与正常组比较，模型组血浆BALF中IL-12，IFN-

水平均显著降低（

0.01），而IL-9，IL-4和IL-13的水平均显著升高（

0.01），IL-4R1，IL-13RA1在模型组细支气管组织中的的表达均显著增强（

0.01）；与模型组比较，二陈汤加味中、高剂量血浆和BALF中的IL-12，IFN-

水平均显著升高（

0.01），而IL-9，IL-4和IL-13的水平均显著降低（

0.01），IL-4R1，IL-13RA1在细支气管组织中的的表达均显著减弱（

0.01）。

结论

二陈汤加味可能通过增加IL-12，IFN-

的释放，减少IL-9，IL-4和IL-13分泌，并抑制IL-4R1和IL-13RA1的表达，起到抗炎和保护细支气管结构的作用。

Abstract

Objective

To study the effect of modified Erchentang on levels of interleukin-12 (IL-12)

interferon-

(IFN-

)

interleukin-9 (IL-9)

interleukin-4 (IL-4) and interleukin-13 (IL-13) in plasma and bronchoalveolar lavage fluid (BALF) of all rats

as well as expressions of interleukin-4 (IL-4) receptor (IL-4R1) and interleukin-13 (IL-13) receptor (IL-13RA1) in bronchioles tissue of rats with chronic obstructive pulmonary disease (COPD).

Method

Fifty SD rats were randomly divided into 5 groups

namely normal group

model group

and low

middle and high-dose modified Erchentang groups (5

20 g·kg

-1

)

with 10 rats in each group. COPD in rat was prepared by using cigarette smoke combined with dripping lipopolysaccharide (LPS) in trachea. After the modeling

normal and model groups were given normal saline solution through intragastric (

) administration

while other groups were given corresponding herbal drugs (5

20 g·kg

-1

) intragastrically (

) for 14 days. The levels of IL-12

IFN-

IL-9

IL-4 and IL-13 in plasma and BALF were detected by Enzyme-linked immunosorbent assay (ELISA) method， and immunohistochemistry (IHC) method was used to detect the expressions of IL-4R1 and IL-13RA1 in bronchioles tissue of all of the groups.

Result

Compared with the normal group

the levels of IL-12 and IFN-

were decreased significantly (

0.01)

but the levels of IL-9

IL-4 and IL-13 in plasma and BALF were significantly increased (

0.01)

and the expressions of IL-4R1 and IL-13RA1 in bronchioles tissue were increased significantly (

0.01) in model group. Compared with the model group

the levels of IL-12 and IFN-

were increased significantly

while the levels of IL-9

IL-4 and IL-13 in plasma and BALF were decreased significantly (

0.01)

and the expressions of IL-4R1 and IL-13RA1 in bronchioles tissue were decreased significantly (

0.01) in modified Erchentang groups (10

20 g·kg

-1

Conclusion

Modified Erchentang has effects in resisting inflammatory and protecting tissue structure of bronchioles. Its mechanism may be correlated with increasing the levels of IL-12

IFN-

and reducing the levels of IL-9

IL-4 and IL-13 in plasma and BALF

and inhibiting the expressions of IL-4R1 and IL-13RA1 in bronchioles tissue.

关键词

Keywords

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