Effect of Huangqi Jianzhongtang on JAK2/STAT3 Signal Pathway in Rats with Spleen-Stomach Deficiency Cold Type Gastric Ulcer

Min BAI; Yong-qiang DUAN; Xiao-yi YANG; Qiang WANG; Qin LI; Zi-han GONG; Jun MA; Ban WANG

doi:10.13422/j.cnki.syfjx.20201908

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Effect of Huangqi Jianzhongtang on JAK2/STAT3 Signal Pathway in Rats with Spleen-Stomach Deficiency Cold Type Gastric Ulcer

Classic Prescriptions | 更新时间：2020-09-16

- Effect of Huangqi Jianzhongtang on JAK2/STAT3 Signal Pathway in Rats with Spleen-Stomach Deficiency Cold Type Gastric Ulcer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 32-38(2020)
- 作者机构：
  
  1.甘肃中医药大学，兰州 730000
  2.敦煌医学与转化教育部重点实验室，兰州 730000
  3.甘肃卫生职业学院，兰州 730000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201908
  CLC： R2-0;R22;R285.5;R289
- Received：16 February 2020，
  
  Published Online：21 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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白敏,段永强,杨晓轶等.黄芪建中汤对脾胃虚寒型胃溃疡模型大鼠JAK2/STAT3信号通路的影响[J].中国实验方剂学杂志,2020,26(20):32-38.

BAI Min,DUAN Yong-qiang,YANG Xiao-yi,et al.Effect of Huangqi Jianzhongtang on JAK2/STAT3 Signal Pathway in Rats with Spleen-Stomach Deficiency Cold Type Gastric Ulcer[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):32-38.
白敏,段永强,杨晓轶等.黄芪建中汤对脾胃虚寒型胃溃疡模型大鼠JAK2/STAT3信号通路的影响[J].中国实验方剂学杂志,2020,26(20):32-38. DOI： 10.13422/j.cnki.syfjx.20201908.

BAI Min,DUAN Yong-qiang,YANG Xiao-yi,et al.Effect of Huangqi Jianzhongtang on JAK2/STAT3 Signal Pathway in Rats with Spleen-Stomach Deficiency Cold Type Gastric Ulcer[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):32-38. DOI： 10.13422/j.cnki.syfjx.20201908.

摘要

目的

探讨黄芪建中汤对脾胃虚寒型胃溃疡（GU）模型大鼠Janus蛋白酪氨酸激酶2(JAK2)/信号转导和转录活化因子3(STAT3)信号通路的影响。

方法

将60只SPF级Wistar大鼠随机分为正常组和模型组，模型组大鼠采用综合造模法复建脾胃虚寒型GU模型，将模型大鼠按随机数字表法分为模型组、安胃疡组和黄芪建中汤高、中、低剂量组，每组10只。正常组和模型组大鼠给予10 mL·kg

-1

·d

-1

蒸馏水灌胃，黄芪建中汤高、中、低剂量组分别给予16，8，4 g·kg

-1

·d

-1

黄芪建中汤灌胃，阳性药组给予0.14 g·kg

-1

·d

-1

安胃疡灌胃，持续治疗21 d。采用酶联免疫吸附测定（ELISA）检测各组大鼠胃组织中白细胞介素-10（IL-10），白细胞介素-17（IL-17）含量，实时荧光定量聚合酶链式反应（Real-time PCR）检测胃组织JAK2，STAT3 mRNA表达水平，蛋白免疫印迹法（Western blot）检测胃组织JAK2，STAT3蛋白表达以及磷酸化水平。

结果

与正常组比较，模型组大鼠一般生存状况相对较差，胃组织匀浆液中IL-10含量明显降低，IL-17含量明显升高（

0.05），胃组织JAK2，STAT3蛋白表达水平升高不明显，而JAK2，STAT3 mRNA表达及蛋白磷酸化水平明显升高（

0.05）；与模型组比较，治疗组大鼠IL-10含量升高，IL-17含量降低，JAK2，STAT3 mRNA表达及蛋白磷酸化水平均降低，其中尤以黄芪建中汤高剂量组明显（

0.05）。

结论

黄芪建中汤具有改善脾胃虚寒型胃溃疡模型大鼠的生存状况的作用，其机制可能与干预JAK2/STAT3通路激活所介导的胃黏膜免疫屏障功能障碍有关。

Abstract

Objective

To investigate the effect of Huangqi Jianzhongtang on Janusprotein tyrosine kinase 2/signal transducers and transcriptional activator protein 3 (JAK2/STAT3) signal pathway in rats with spleen-stomach deficiency cold type gastric ulcer (GU).

Method

A total of 60 SPF level Wistar rats were randomly divided into two groups: blank group and model group. Model rats were used to reconstruct the spleen-stomach deficiency cold type GU model by comprehensive modeling method. Model rats were divided into model group

Anweiyang group and high

medium and low-dose Huangqi Jianzhongtang groups according to the random number table

with 10 rats in each group. Rats in blank group and model group were given 10 mL·kg

-1

·d

-1

distilled water

and 16

4 g·kg

-1

·d

-1

Huangqi Jianzhongtang

respectively. Rats in the positive control group were given 0.14 g·kg

-1

·d

-1

Anweiyang for 21 days. The gene expressions of JAK2 and STAT3 in the ulcer tissue were detected by Real-time fluorescent quantitative polymerase chain reaction (Real-time PCR)

the protein expressions and phosphorylation levels of JAK2 and STAT3 in the ulcer tissue were detected by Western blot

and the contents of interleukin-10（IL-10）and interleukin-17（IL-17）in the gastric tissue of each group were detected by enzyme-linked immunosorbent assay (ELISA).

Result

Compared with the blank group

the general survival condition of the model group was worse

the content of IL-10 in gastric homogenate was significantly reduced

while the content of IL-17 was significantly increased (

0.05)

the protein expressions of JAK2 and STAT3 in gastric tissue was not significantly increased

whereas the gene expressions and phosphorylation levels of JAK2 and STAT3 were significantly increased (

0.05). Compared with the model group

the content of IL-10 increased

but the content of IL-17 decreased

the gene expressions of JAK2 and STAT3 and the level of protein phosphorylation decreased in the treatment group

especially in the high-dose Huangqi Jianzhongtang group

with statistically significant differences (

0.05).

Conclusion

Huangqi Jianzhongtang can improve the survival condition of rats with spleen stomach deficiency cold type gastric ulcer

and its mechanism may be related to the intervention of gastric mucosal immune barrier dysfunction mediated by JAK2/STAT3 pathway activation.

关键词

Keywords

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