Objective

2

To establish ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for the analysis of 18 components of five categories

namely spirosteroid saponins （timosaponin AⅠ，timosaponin AⅡ，timosaponin AⅢ，anemarrhenasaponin Ⅲ，sarsasapogenin），furostane saponins（anemarrhenasaponin Ⅰ，anemarrhenasaponin Ⅰa，anemarsaponin E，officinalisinin I，timosaponin B-Ⅱ，timosaponin B-Ⅲ，anemarsaponin C），flavonoids（icarisin I，baohuoside I），bisphenone（meomangiferin，mangiferin，isomangiferin），and hydroquinone glycoside （

β

-arbutin）

in order to analyze the differences between the main root and fibrous root of

Anemarrhena asphodeloides

from different sources and provide reference for the sustainable development of

A. asphodeloides

resources.

Method

2

0.25 g sample was refluxed and extracted with 25 mL dilute ethanol for 30 min. The chromatographic separation was carried out on Waters Acquity Uplc BEHHILI C

18

column（2.1 mm×100 mm，1.7 μm），with 0.1% formic acid water-acetonitrile as the mobile phase for gradient elution，and the volume flow rate was 0.2 mL·min

-1

. Electrospray ion source（ESI

+

，ESI

-

），and multi-reaction ion monitoring（MRM） were used for detection，external standard method was used to calculate the content of the tested components in medicinal samples，and SMICA14.1 software was used to analyze the differences between the main root and fibrous root samples of

A. asphodeloides.

Result

2

The tested components showed a good linear relationship in their respective linear ranges，with a good precision，repeatability and stability. The recovery rate of samples was between 95.22%-101.42%，and RSD was less than 4%. The experimental results showed great differences between the main root and fibrous root of

A. asphodeloides

when the multivariate statistical analysis was carried out with 18 main components.

Conclusion

2

This study provides experimental basis for the reuse of fibrous root of

A. asphodeloides

resources and the quality control of

A. asphodeloides.