Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM Rats

doi:10.13422/j.cnki.syfjx.20201937

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Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM Rats

Classic Prescriptions | 更新时间：2020-09-16

- Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 19, Pages: 114-119(2020)
- 作者机构：
  
  1.湖北中医药大学，武汉 430065
  2.山西中医药大学，太原 030024
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201937
  CLC： R2-0;R289;R587.1
- Received：12 March 2020，
  
  Published Online：30 July 2020，
  
  Published：05 October 2020
- 稿件说明：
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王超群,王永辉,刘涛等.左归丸对妊娠糖尿病大鼠血清相关指标及胰腺PDX-1表达的影响[J].中国实验方剂学杂志,2020,26(19):114-119.

.Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):114-119.
王超群,王永辉,刘涛等.左归丸对妊娠糖尿病大鼠血清相关指标及胰腺PDX-1表达的影响[J].中国实验方剂学杂志,2020,26(19):114-119. DOI： 10.13422/j.cnki.syfjx.20201937.

.Effect of Zuoguiwan on Serum Related Indexes and Pancreatic PDX-1 Expression in GDM Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):114-119. DOI： 10.13422/j.cnki.syfjx.20201937.

摘要

目的

研究左归丸母代干预对妊娠糖尿病（GDM）模型大鼠血清及以胰腺指标改善作用的影响。

方法

通过雌雄同笼及腹腔注射链脲佐菌素（STZ）的方法复制GDM孕鼠模型，采用随机数字表法将孕鼠分为正常组，模型组，门冬胰岛素组（20 U·kg

-1

），左归丸低、中、高剂量组（2.5，5.0，10.0 g·kg

-1

）。各组分别灌服相应药物2周，正常组和模型组予以等体积生理盐水，采用酶联免疫吸附测定（ELISA）检测大鼠血清胰岛素，空腹血糖（FBG），及炎性因子肿瘤坏死因子-

（TNF-

），白细胞介素-6（IL-6），白细胞介素-8（IL-8）的水平；蛋白免疫印迹法（Western blot），实时荧光定量聚合酶链式反应（Real-time PCR）检测大鼠胰腺十二指肠同源盒因子-1（PDX-1）蛋白及mRNA的表达水平。

结果

与正常组比较，模型组大鼠空腹血糖及炎性因子TNF-

，IL-6均显著升高（

0.01），IL-8水平明显升高（

0.05），大鼠血清胰岛素水平、胰腺PDX-1蛋白及mRNA表达均显著降低（

0.01）；与模型组比较，门冬胰岛素组血糖及血清炎性因子IL-8水平显著降低（

0.01），血清胰岛素水平、胰腺PDX-1蛋白及mRNA表达均显著升高（

0.01），左归丸中剂量组血糖及炎性因子TNF-

，IL-6，IL-8显著降低（

0.01），血清胰岛素水平、胰腺PDX-1蛋白及mRNA表达均显著升高（

0.01）。

结论

左归丸可能通过降低GDM大鼠血清炎性因子水平，减少炎症反应及氧化应激反应的发生，以提升胰岛素的分泌，同时上调PDX-1蛋白和mRNA的表达，改善胰岛素抵抗，减少胰岛

细胞的损伤，降低大鼠血糖。

Abstract

Objective

To investigate the effect of maternal intervention of Zuoguiwan on the improvement of serum and pancreatic index in rats of gestational diabetes mellitus(GDM).

Method

The GDM pregnant rats were replicated by monogamous cage and introneal injection of streptozotocin (STZ). The pregnant rats were randomly divided into normal group

model group

insulin group (20 U·kg

-1

)

and low

medium and high-dose Zuoguiwan groups (2.5，5.0，10.0 g·kg

-1

). The insulin group was given hypodermic injection of portal winter insulin

all drug groups were respectively administrated with corrsponding drugs for 2 weeks

once a day. Meanwhile

control group and model group were given physiological saline by gavage. After that

the levels of serum insulin

fasting plasma glucose（FBG）

tumor necrosis factor-

(TNF-

)

interleukin-6 (IL-6) and IL-8 were measured by enzyme-linked immunosorbent assay (ELISA)

protein and gene expression levels of Pancreas-duodenal homologous box factor-1 (PDX-1) were respectively detected by Western blot and real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）.

Result

Compared with normal group

FBG

TNF-

and IL-6 levels of pregnant rats in model group increased significantly (

0.01)

the level of IL-8 increased uniformly (

0.05)

and serum insulin level

protein and gene expression levels of PDX-1 decreased significantly (

0.01). Compared with model group

FBG and serum IL-8 levels of pregnant rats in insulin group were significantly lower (

0.01)

while serum insulin level

protein and gene expression levels of PDX-1 increased significantly(

0.01). FBG

IL-8

IL-6 and TNF-

levels in serum of pregnant rats in medium-dose Zuoguiwan group decreased obviously

meanwhile serum insulin level

protein and gene expression levels of PDX-1 increased significantly (

0.01).

Conclusion

Zuoguiwan may promote the secretion of insulin by reducing the serum inflammatory factors in GDM rats

and at the same time up-regulate the expression of PDX-1 protein and gene

so as to restrain insulin resistance

reduce the damage of pancreatic cells and improve the blood glucose of rats.

关键词

Keywords

references

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