Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-<italic style="font-style: italic">β</italic><sub>1</sub>/Smad Pathway

You-hui KE; Chen KE; Zhi-han WANG; Ming YANG; Wen-ting SU; Jian-xun REN; Na LANG

doi:10.13422/j.cnki.syfjx.20201938

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Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β₁/Smad Pathway

Pharmacology | 更新时间：2020-09-16

- Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β₁/Smad Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 19, Pages: 169-176(2020)
- 作者机构：
  
  1.浙江中医药大学附属温州中西医结合医院，浙江温州 325000
  2.温州医科大学第二临床学院，浙江温州 325000
  3.中国中医科学院西苑医院，北京 100091
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201938
  CLC： R2-0;R289;R364.5
- Received：26 April 2020，
  
  Published Online：29 July 2020，
  
  Published：05 October 2020
- 稿件说明：
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柯友辉,柯晨,王志瀚等.山豆根醇提取物干预TGF-β₁/Smad通路抑制兔耳增生性瘢痕的机制[J].中国实验方剂学杂志,2020,26(19):169-176.

KE You-hui,KE Chen,WANG Zhi-han,et al.Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β₁/Smad Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):169-176.
柯友辉,柯晨,王志瀚等.山豆根醇提取物干预TGF-β₁/Smad通路抑制兔耳增生性瘢痕的机制[J].中国实验方剂学杂志,2020,26(19):169-176. DOI： 10.13422/j.cnki.syfjx.20201938.

KE You-hui,KE Chen,WANG Zhi-han,et al.Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β₁/Smad Pathway[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):169-176. DOI： 10.13422/j.cnki.syfjx.20201938.

摘要

目的

研究山豆根醇提取物对兔耳部增生性瘢痕组织中转化生长因子-

（TGF-

）和Smad3表达的影响，探讨山豆根醇提取物改善增生性瘢痕的作用与机制。

方法

通过损伤新西兰大耳白兔耳内侧皮肤建立兔耳增生性瘢痕模型。49只大耳白兔随机分为空白组、模型组，山豆根醇提取物高、中、低剂量组（2.0，1.0，0.4 g·kg

-1

），积雪苷软膏组（5 mg·kg

-1

）以及复方肝素钠尿囊素凝胶组（20 mg·kg

-1

），7只/组。除空白组外，其余各组在模型建立后分别采用相应的药物涂抹于兔耳增生性瘢痕处，1次/日，连续给药42 d。实验结束后取耳部瘢痕组织，采用苏木素-伊红（HE）染色观察兔耳瘢痕组织病理改变并测定瘢痕增生指数；分别采用免疫组化，蛋白免疫印迹法（Western blot）以及逆转录聚合酶链式反应（RT-PCR）检测瘢痕组织中TGF-

和Smad3蛋白及mRNA的表达。

结果

与空白组比较，模型组动物耳瘢痕组织病理显示增生明显，增生指数显著增加（

0.01）；同时瘢痕组织中TGF-

和Smad3表达也显著升高（

0.01）；与模型组比较，山豆根醇提取物中、高剂量组耳部瘢痕组织病理结构明显改善，瘢痕组织TGF-

和Smad3表达及增生指数明显下降（

0.05，

0.01），山豆根醇提取物各剂量组TGF-

和Smad3蛋白与mRNA表达明显下降（

0.05，

0.01）。

结论

山豆根醇提取物可能通过降低瘢痕组织中TGF-

和Smad3表达，抑制TGF-

/Smads信号转导通路发挥抑制增生性瘢痕的疗效，这为山豆根治疗增生性瘢痕的临床应用提供了实验基础。

Abstract

Objective

To investigate that the effect of ethanol extracts from Sophorae Tonkinensis Radix et Rhizoma on the expression of transforming growth factor-

(TGF-

)and Smad3 in the hypertrophic scars of rabbit ears and elucidate its mechanism to improve hypertrophic scars.

Method

The model of hypertrophic ear scar model was established by damaging the inner skin of ears in New Zealand white rabbits.The 49 rabbits were randomly divided into control group

model group

low

medium and high-dose ethanol extracts groups from Sophorae Tonkinensis Radix et Rhizoma (0.4，1.0，2.0 g·kg

-1

)

asiaticoside ointment group(5 mg·kg

-1

) and compound heparin sodium allantoin gel group(20 mg·kg

-1

)

7 rabbits per group. Except control group

the different drug about 0.5 mL had been applied the hypertrophic scar of rabbit ears once a day. After 42 days

the tissues of hypertrophic scar were obtained. Hematoxylin-eosin（HE）staining was used to observe the pathological changes of rabbit ear scar tissue and determine the scar hyperplasia index. The expression of TGF-

and Smad3 in scar tissue of rabbit ears were detected by immunohistochemistry

Western blot and reverse transcription PCR（RT-PCR）.

Result

Compared with control group

the pathological changes of the ear scars in the model group showed obvious hyperplasia and higher hyperplasia index (

0.01). Meanwhile

the expressions of TGF-

and Smad3 in scar tissue of rabbit ears were significantly increased (

0.01). Compared with model group

the pathological structures of the ear scar tissue were significantly improved and the hyperplasia index of ear scar tissue was clearly reduced in medium and high-dose groups of ethanol extracts from Sophorae Tonkinensis Radix et Rhizoma(

0.05，

0.01). The protein and mRNA expression of TGF-

and Smad3 in scar tissue were also decreased in different group of ethanol extracts from Sophorae Tonkinensis Radix et Rhizoma compared with the model group (

0.05，

0.01).

Conclusions

Ethanol extracts from Sophorae Tonkinensis Radix et Rhizoma may play a curative role in inhibiting hypertrophic scars by reducing the expression of TGF-

and Smad3 in scar tissue and inhibiting the TGF-

/Smads signal transduction pathway. These provides the experimental basis for the clinical application of Sophorae Tonkinensis Radix et Rhizoma in the treatment of hypertrophic scars.

关键词

Keywords

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Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β1/Smad Pathway

DOI：10.13422/j.cnki.syfjx.20201938

摘要

Abstract

关键词

Keywords

references

Mechanism of Ethanol Extracts from Sophorae Tonkinensis Radix et Rhizoma on Hypertrophic Scars of Rabbit Ears Through TGF-β₁/Smad Pathway