Intervention of Shenma Yizhi Prescription on Learning and Memory in Rats with Vascular Cognitive Impairment Induced by Chronic Cerebral Ischemia

Cheng-cheng SUN; Jian-gang LIU; Mei-xia LIU; Zeng-gang LUO; Hao LI

doi:10.13422/j.cnki.syfjx.20201940

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Intervention of Shenma Yizhi Prescription on Learning and Memory in Rats with Vascular Cognitive Impairment Induced by Chronic Cerebral Ischemia

Pharmacology | 更新时间：2020-09-16

- Intervention of Shenma Yizhi Prescription on Learning and Memory in Rats with Vascular Cognitive Impairment Induced by Chronic Cerebral Ischemia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 19, Pages: 153-159(2020)
- 作者机构：
  
  1.中国中医科学院西苑医院，北京 100091
  2.中国中医科学院研究生院，北京 100700
  3.中国中医科学院老年病研究所，北京 100091
  4.北京市中医药管理局，北京 100053
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201940
  CLC： R2-0;R289;R743
- Received：15 February 2020，
  
  Published Online：29 July 2020，
  
  Published：05 October 2020
- 稿件说明：
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孙成成,刘剑刚,刘美霞等.参麻益智方对慢性脑缺血致血管性认知障碍大鼠学习记忆功能的干预作用[J].中国实验方剂学杂志,2020,26(19):153-159.

SUN Cheng-cheng,LIU Jian-gang,LIU Mei-xia,et al.Intervention of Shenma Yizhi Prescription on Learning and Memory in Rats with Vascular Cognitive Impairment Induced by Chronic Cerebral Ischemia[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):153-159.
孙成成,刘剑刚,刘美霞等.参麻益智方对慢性脑缺血致血管性认知障碍大鼠学习记忆功能的干预作用[J].中国实验方剂学杂志,2020,26(19):153-159. DOI： 10.13422/j.cnki.syfjx.20201940.

SUN Cheng-cheng,LIU Jian-gang,LIU Mei-xia,et al.Intervention of Shenma Yizhi Prescription on Learning and Memory in Rats with Vascular Cognitive Impairment Induced by Chronic Cerebral Ischemia[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(19):153-159. DOI： 10.13422/j.cnki.syfjx.20201940.

摘要

目的

观察参麻益智方对双侧颈总动脉结扎造成慢性脑缺血致血管性认知障碍模型大鼠的学习记忆能力和血清炎症因子及氧化应激相关指标的影响。

方法

SPF级SD大鼠，采用双侧颈总动脉结扎法制备慢性脑缺血模型，手术成功后筛选认知障碍的大鼠按随机数字表法分成5组，模型组、盐酸多奈哌齐组（0.45 mg·kg

-1

），参麻益智方低、中、高剂量组（2.97，5.94，11.88 g·kg

-1

），另设假手术组，每组10只。给药方式为灌胃，其中模型组和假手术组给予等体积纯净水灌胃，连续灌胃8周后进行相关指标检测。采用Morris水迷宫实验进行大鼠学习记忆功能检测；苏木素-伊红（HE）染色观察海马CA1区病理形态学改变；比色法检测血清中乙酰胆碱（Ach），乙酰胆碱酯酶（AChE）含量；酶联免疫吸附测定（ELISA）检测大鼠血清炎症因子白细胞介素-1

（IL-1

）和肿瘤坏死因子-

（TNF-

）含量；比色法检测谷胱甘肽（GSH），超氧化物歧化酶（SOD），丙二醛（MDA）含量；非酶促免疫法检测谷胱甘肽过氧化物还原酶（GSH-Px）含量。

结果

与假手术组比较，模型组大鼠逃避潜伏期均明显增加，寻台次数、穿越原平台次数、原平台象限游泳时间占总游泳时间百分比均明显减少（

0.05，

0.01），Ach，GSH，GSH-Px含量均明显减少，AChE，IL-1

，TNF-

，MDA含量均明显增加（

0.05，

0.01）

）

；与模型组比较，参麻益智方低、中、高剂量组大鼠逃避潜伏期均显著减少，大鼠寻台次数、穿越原平台次数、原平台象限游泳时间占总游泳时间百分比均明显增加（

0.05，

0.01），参麻益智方中、高剂量组AChE含量显著减少（

0.01），参麻益智方高剂量组大鼠血清Ach含量明显增加（

0.05），大鼠血清IL-1

，TNF-

，MDA含量均明显减少（

0.05，

0.01），GSH，SOD，GSH-Px含量明显增加（

0.05，

0.01）。

结论

参麻益智方可改善双侧颈总动脉结扎引起慢性脑缺血致血管性认知障碍大鼠的学习记忆能力，并具有改善其海马的病理形态，保护神经元，增强胆碱能系统功能，抑制炎症反应，提高机体抗氧化能力。

Abstract

Objective

To observe the effects of Shenma Yizhi prescription（SMYZ）on learning and memory， inflammatory factors and oxidative stress related indexes of rats with chronic cerebral ischemia inducing vascular dementia caused by bilateral common carotid artery ligation.

Method

The chronic cerebral ischemia model of SPF grade SD rats was prepared by bilateral common carotid artery ligation. After successful surgery， the rats with cognitive impairment were randomly divided into 5 groups，model， donepezil（0.45 mg·kg

-1

）， SMYZ-low， medium and high dose（2.97，5.94，11.88 g·kg

-1

）， with 10 rats in each group， and 10 rats in sham operation group were established. Intragastric administration were given for 8 weeks respectively then followed Morris water maze test. The pathomorphological changes of hippocampus CA1 were observed. The serum levels of acetylcholine(Ach)and acetylcholinesterase (AChE)were detected， and the levels of inflammatory cytokines interleukin-1

(IL-1

) and tumor necrosis factor-

(TNF-

) in the serum of rats were detected. The contents of glutathione (GSH)， malondialdehyde (MDA)， superoxide dismutase (SOD) and glutathione peroxidase reductase (GSH-Px) were observed.

Result

Compared with sham operation group， the escape latency increased significantly in the model group， and the frequency of platform search， the frequency of crossing the original platform and the percentage of swimming time in the original platform quadrant decreased significantly （

0.05，

0.01）. The contents of Ach and the contents of GSH and GSH-Px were significantly decreased （

0.05，

0.01）. The contents of AChE， IL-1

and TNF-

and the contents of MDA were significantly increased （

0.05，

0.01）. Compared with model group， the escape latency of rats in the in the low， medium and high dose groups of SMYZ prescription was significantly reduced （

0.05，

0.01）.The frequency of looking for a platform， the frequency of crossing the original platform and the percentage of swimming time in the original platform quadrant of the rats in the low， medium and high dose groups of SMYZ prescription increased significantly （

0.05，

0.01）. The content of AChE in high dose group were significantly reduced（

0.01）， and the content of Ach in high dose group were significantly increased（

0.05）， the rat serum IL-1

， TNF-

levels and MDA content were significantly reduced（

0.05，

0.01）. The GSH， SOD and GSH-Px levels were significantly increased （

0.05，

0.01）.

Conclusion

SMYZ can improve the learning and memory ability of rats with vascular cognitive disorder caused by chronic cerebral ischemia caused by ligation of bilateral common carotid artery， bilateral common carotid artery ligation， improve the pathological morphology of hippocampal tissue， inhibit inflammatory response， improve cholinergic level and oxidative stress state.

关键词

Keywords

references

LIU D D ， YUAN X ， CHU S F ， et al . CZ-7，a new derivative of Claulansine F，ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses [J]. Acta Pharmacol Sin ， 2018 ， 40 : 425 - 440 .

DICHGANS M ， LEYS D . Vascular cognitive impairment [J]. Circ Res ， 2017 ， 3 : 573 - 591 .

DAMODARAN T ， MULLER C P ， HASSAN Z . Chronic cerebral hypoperfusion-induced memory impairment and hippocampal long-term potentiation deficits are improved by cholinergic stimulation in rats [J]. Pharmacol Rep ， 2019 ， 71 （ 3 ）: 443 - 448 .

SUN M K . Potential therapeutics for vascular cognitive impairment and dementia [J]. Curr Neuropharmacol ， 2018 ， 7 : 1036 - 1044 .

KALARIA R N . The pathology and pathophysiology of vascular dementia [J]. Neuropharmacology ， 2018 ， 134 : 226 - 239 .

HASE Y ， DING R ， HARRISON G ， et al . White matter capillaries in vascular and neurodegenerative dementias [J]. Acta Neuropathol Commun ， 2019 ， 7 （ 1 ）: 16 .

JIANG P ， CHEN L ， SUN J ， et al . Chotosan ameliorates cognitive impairment and hippocampus neuronal loss in experimental vascular dementia via activating the Nrf2-mediated antioxidant pathway [J]. J Pharmacol Sci ， 2019 ， 2 : 105 - 111 .

OKAMOTO K ， TANAKA M T ， KONDO S . Treatment of vascular dementia [J]. Ann N Y Acad Sci ， 2002 ， 977 : 507 - 512 .

吴琼，刘方，刘剑刚，等 . 参麻益智方对轻中度血管性痴呆病人认知功能和血液流变性的影响 [J]. 中西医结合心脑血管病杂志， 2017 ， 15 ( 19 ): 2381 - 2385 .

罗增刚，周文泉，高普，等 . 参麻益智胶囊治疗老年血管性痴呆的临床研究 [J]. 中医杂志， 2001 ， 42 （ 8 ）： 470 - 473 .

曹宇，刁雪梅，李泽惠，等 . 参麻益智方对双侧颈动脉结扎大鼠海马认知功能的影响 [J]. 中西医结合心脑血管病杂志， 2019 ， 17 ( 8 ): 1151 - 1154 .

ZHANG Y ， LIU J ， YANG B ， et al . Ginkgo biloba extract inhibits astrocytic lipocalin-2 expression and alleviates neuroinflammatory injury via the JAK2/STAT3 pathway after ischemic brain stroke [J]. Front Pharmacol ， 2018 ， 9 ： 518 .

谢秀琼，主编 . 中药新制剂开发与应用 [M]. 2版 . 北京：人民卫生出版社， 2008 : 421 - 422 .

CAO Y ， LIANG L ， XU J ， et al . The effect of Scutellaria baicalensis stem-leaf flavonoids on spatial learning and memory in chronic cerebral ischemia-induced vascular dementia of rats [J]. Acta Biochim Biophys Sin ， 2016 ， 48 ( 5 ): 437 - 446 .

孙成成，刘剑刚，刘美霞，等 . 慢性脑低灌注致血管性痴呆的病理机制和模型建立 [J]. 神经药理学报， 2019 ， 9 ( 1 ): 13 - 17 .

GE J F ， QI C C ， QIAO J P ， et al . Sex differences in ICR mice in the Morris water maze task [J]. Physiol Res ， 2013 ， 62 （ 1 ）: 107 - 117 .

MAGUIRE E A ， INTRAUB H ， MULLALLY S L . Scenes，spaces，and memory traces:what does the hippocampus do? [J]. Neuroscientist ， 2016 ， 22 （ 5 ）: 432 - 439 .

YU J Y ， FANG P ， WANG C ， et al . Dorsal CA1 interneurons contribute to acute stress-induced spatial memory deficits [J]. Neuropharmacology ， 2018 ， 135 : 474 - 486 .

PICCIOTTO M R ， HIGLEY M J ， MINEUR Y S . Acetylcholine as a neuromodulator:cholinergic signaling shapes nervous system function and behavior [J]. Neuron ， 2012 ， 76 （ 1 ）: 116 - 129 .

HAAM J ， YAKEL J L . Cholinergic modulation of the hippocampal region and memory function [J]. J Neurochem ， 2017 ， 2 : 111 - 121 .

DING H G ， DENG Y Y ， YANG R Q ， et al . Hypercapnia induces IL-1beta overproduction via activation of NLRP3 inflammasome:implication in cognitive impairment in hypoxemic adult rats [J]. J Neuroinflammation ， 2018 ， 15 （ 1 ）: 4 .

PARK J C ， HAN S H ， MOOK-JUNG I . Peripheral inflammatory biomarkers in Alzheimer's disease:a brief review [J]. BMB Rep ， 2020 ， 53 （ 1 ）: 10 - 19 .

TONNIES E ， TRUSSHINA E . Oxidative stress，synaptic dysfunction，and Alzheimer's disease [J]. J Alzheimers Dis ， 2017 ， 57 （ 4 ）: 1105 - 1121 .

QIN Y ， FAN J ， YANG W ， et al . An endogenous Cys-assisted GSH@AgNCs-rGO nanoprobe for real-time monitoring of dynamic change in GSH levels regulated by natural drug [J]. Anal Chem ， 2020 ， 92 （ 2 ）: 1988 - 1996 .

LAN R ， LI S ， CHANG Q ， et al . Chitosan oligosaccharides protect sprague dawley rats from cyclic heat stress by attenuation of oxidative and inflammation stress [J]. Animals (Basel) ， 2019 ， 9 ( 12 ): 1074 .

CUI X ， GONG J ， HAN H ， et al . Relationship between free and total malondialdehyde，a well-established marker of oxidative stress，in various types of human biospecimens [J]. J Thorac Dis ， 2018 ， 10 （ 5 ）: 3088 - 3097 .

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