Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin

Xin HU; Xu WANG; Man LIU; Hong PEI; Zhi-guo ZHU; Bi-sheng HUANG; Yi-fei LIU; Cheng-wu SONG; Zhi-gang HU

doi:10.13422/j.cnki.syfjx.20201947

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Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin

Pharmacy Fundamentals | 更新时间：2020-09-16

- Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 132-139(2020)
- 作者机构：
  
  1.湖北中医药大学药学院，武汉 430065
  2.广州康和药业有限公司，广州 511440
  3.湖北金贵中药饮片有限公司，武汉 430051
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201947
  CLC： R22;R931;R28;Q523;O657.7
- Received：16 January 2020，
  
  Published Online：16 June 2020，
  
  Published：20 October 2020
- 稿件说明：
移动端阅览
胡鑫,王旭,刘曼等.16个产地地乌药材的系统鉴定及质量评价[J].中国实验方剂学杂志,2020,26(20):132-139.

HU Xin,WANG Xu,LIU Man,et al.Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):132-139.
胡鑫,王旭,刘曼等.16个产地地乌药材的系统鉴定及质量评价[J].中国实验方剂学杂志,2020,26(20):132-139. DOI： 10.13422/j.cnki.syfjx.20201947.

HU Xin,WANG Xu,LIU Man,et al.Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):132-139. DOI： 10.13422/j.cnki.syfjx.20201947.

摘要

目的

构建地乌药材基原物种系统鉴定体系，并对全国16个产地的地乌药材进行综合品质评价，为地乌药材产地选择及临床用药安全奠定基础。

方法

使用传统鉴别方法结合DNA条形码核糖体DNA第二内部转录间隔区（ITS2）序列分子鉴定技术快速鉴别地乌药材真伪，并基于HPLC-UV对地乌药材中5个有效成分进行含量测定，采用Welch Ultimate XB-C

色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.01%三氟乙酸溶液（30∶70），检测波长210 nm，柱温30 ℃，流速1.0 mL·min

-1

。

结果

传统鉴别及DNA条形码快速鉴别技术均能准确鉴别地乌药材真伪。BLAST比对分析发现，16个产地地乌药材均与林荫银莲花

Anemone flaccida

具有最大相似度；基于多指标成分含量测定表明湖北恩施板桥镇的地乌药材中5个三萜皂苷类成分含量之和最高（10.59%），其次为贵州毕节赫章（6.28%）和湖北长阳都镇湾（5.64%）。

结论

DNA条形码技术可作为地乌药材传统鉴定技术的有效补充，该鉴别体系可保障地乌药材基原准确及临床用药安全。HPLC多指标成分综合评价及聚类分析结果表明在本研究所涉及的产地中，湖北恩施、长阳、五峰，贵州毕节和重庆金佛山的地乌药材质量较优，可作为地乌药材的重要产地。

Abstract

Objective

To construct a systematic identification system of Anemonis Flaccidae Rhizoma

and to evaluate the comprehensive quality of Anemonis Flaccidae Rhizoma from 16 regions in China

so as to lay a foundation for its origin selection and clinical medication safety.

Method

The authenticity of Anemonis Flaccidae Rhizoma was quickly identified by traditional identification method and DNA barcode molecular identification technology

and HPLC-UV was used to determine the contents of 5 active ingredients in Anemonis Flaccidae Rhizoma. All high pressure chromatographic separations were performed with a Welch Ultimate XB-C

column (4.6 mm×250 mm

5 μm)

the mobile phase consisted of acetonitrile-0.01% trifluoroacetic acid aqueous solution (30∶70) at a flow rate of 1.0 mL·min

-1

. The detection wavelength was set at 210 nm and the column temperature was maintained at 30 ℃.

Result

The authenticity of Anemonis Flaccidae Rhizoma could be precisely and rapidly identified by ribosomal DNA internal transcribed spacer 2 (ITS2) sequence and traditional identification methods. BLAST comparative analysis found that medicinal materials from 16 areas were all

Anemone flaccida

. Based on the contents of multi-index components

it was shown that the total content of 5 triterpenoid saponins in Anemonis Flaccidae Rhizoma from Banqiao

Enshi

Hubei was the highest (10.59%)

followed by Hezhang

Bijie

Guizhou (6.28%) and Duzhenwan

Changyang

Hubei (5.64%).

Conclusion

DNA barcoding can be used as an effective supplement to the traditional identification technology

it can ensure the authenticity of Anemonis Flaccidae Rhizoma and the safety of clinical use. The comprehensive evaluation of multi-index components of HPLC and cluster analysis show that the quality of medicinal materials in Enshi

Changyang

Wufeng of Hubei

Bijie of Guizhou and Jinfoshan of Chongqing is superior

which can be considered as important origin of Anemonis Flaccidae Rhizoma.

关键词

Keywords

references

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