Effect of Wenjing Huayu Zhitong Therapy in Treating Primary Dysmenorrhea and Its Regulatory Mechanisms on T Cell Subsets

Dan-dan WANG; Dong-mei WANG; Ping-ping CAI; Ying-ying MENG; Feng-ting ZHAI

doi:10.13422/j.cnki.syfjx.20201963

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Effect of Wenjing Huayu Zhitong Therapy in Treating Primary Dysmenorrhea and Its Regulatory Mechanisms on T Cell Subsets

Clinic | 更新时间：2020-09-16

- Effect of Wenjing Huayu Zhitong Therapy in Treating Primary Dysmenorrhea and Its Regulatory Mechanisms on T Cell Subsets
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 20, Pages: 94-99(2020)
- 作者机构：
  
  1.山东中医药大学，济南 250355
  2.山东中医药大学附属医院，济南 250014
  3.山东省立医院，济南 250021
  4.青岛西海岸新区中医医院，山东青岛 266500
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20201963
  CLC： R22;R242;R2-031;R287
- Received：27 December 2019，
  
  Published Online：23 July 2020，
  
  Published：20 October 2020
- 稿件说明：
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王丹丹,王东梅,蔡平平等.温经化瘀止痛法治疗原发性痛经的临床疗效及其对T细胞亚群的调控机制[J].中国实验方剂学杂志,2020,26(20):94-99.

WANG Dan-dan,WANG Dong-mei,CAI Ping-ping,et al.Effect of Wenjing Huayu Zhitong Therapy in Treating Primary Dysmenorrhea and Its Regulatory Mechanisms on T Cell Subsets[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):94-99.
王丹丹,王东梅,蔡平平等.温经化瘀止痛法治疗原发性痛经的临床疗效及其对T细胞亚群的调控机制[J].中国实验方剂学杂志,2020,26(20):94-99. DOI： 10.13422/j.cnki.syfjx.20201963.

WANG Dan-dan,WANG Dong-mei,CAI Ping-ping,et al.Effect of Wenjing Huayu Zhitong Therapy in Treating Primary Dysmenorrhea and Its Regulatory Mechanisms on T Cell Subsets[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(20):94-99. DOI： 10.13422/j.cnki.syfjx.20201963.

摘要

目的

观察温经化瘀止痛法对寒凝血瘀证原发性痛经（primary dysmenorrhea，PD）的临床疗效，探讨温经化瘀止痛法治疗PD的免疫机制。

方法

收集寒凝血瘀证PD患者108例，按随机数字表随机分为中药组、布洛芬组和安慰剂组，每组各36例。所有患者均于月经来潮前3 d开始给药，中药组给予中药和布洛芬缓释胶囊模拟剂治疗，布洛芬组给予布洛芬缓释胶囊和中药模拟剂治疗，安慰剂组给予中药模拟剂和布洛芬缓释胶囊模拟剂治疗，连续治疗6 d，共治疗3个月经周期，停药后随访3个月经周期。观察记录各月经期间的视觉模拟评分（visual analogue score，VAS），腹痛累计时间及中医症状，流式细胞术检测患者治疗前后外周血CD3

，CD4

，CD8

水平及CD4

/CD8

。

结果

治疗3个月经周期后，中药组和布洛芬组在降低VAS和减少腹痛累计时间方面均优于安慰剂组（

0.01）；停药后随访远期疗效，中药组显著优于布洛芬组（

0.01）。中药组降低中医症状积分总有效率为91.43%（32/35），布洛芬组总有效率为66.67%（22/33），安慰剂组总有效率为30.30%（10/33），中药组疗效优于布洛芬组（

=-2.971，

0.01），布洛芬组优于安慰剂组（

=-2.371，

0.05）。与本组治疗前比较，治疗后中药组CD3

，CD4

水平及CD4

/CD8

显著升高，CD8

水平显著降低（

0.01）；与布洛芬组和安慰剂组治疗后比较，治疗后中药组CD4

水平及CD4

/CD8

升高（

0.05，

0.01），CD8

水平降低（

0.01）。

结论

温经化瘀止痛法能够显著减少PD患者的VAS评分和腹痛累计时间，改善痛经症状，逆转T细胞亚群失调可能是其治疗寒凝血瘀证PD的作用机制之一。

Abstract

Objective

To observe the clinical efficacy of Wenjing Huayu Zhitong therapy in treating primary dysmenorrhea (PD) with cold coagulation and blood stasis

and to explore its immune mechanisms on PD.

Method

The 108 PD patients with cold coagulation and blood stasis syndrome were collected and randomly divided into traditional Chinese medicine (TCM) group

ibuprofen group and placebo group according to the random number table method

with 36 cases in each group. All patients received corresponding medicines three days before menstruation. The patients in TCM group were treated with TCM and ibuprofen sustained release capsule simulator. The patients in ibuprofen group were treated with ibuprofen sustained-release capsule and TCM simulator. The patients in placebo group were treated with TCM simulator and ibuprofen sustained-release capsule simulator. Treatment lasted for 6 consecutive days for three menstrual cycles

and follow-up was conducted for three menstrual cycles after drug withdrawal. The visual analogue score (VAS)

total time of abdominal pain and TCM symptom scores in each menstrual cycle were recorded. The levels of CD3

CD4

CD8

and the ratio of CD4

/CD8

in peripheral blood before and after treatment were detected by flow cytometry.

Result

After treatment for three menstrual cycles

both the TCM group and ibuprofen group were better than placebo group in reducing VAS score and reducing total abdominal pain time (

0.01). The long-term follow-up effect after drug withdrawal in TCM group was significantly better than that in ibuprofen group (

0.01). The total effective rate was 91.43% (32/35) in TCM group

66.67% (10/33) in ibuprofen groups

and 30.30% (10/33) in placebo group . The efficacy of the TCM group was better than that of the ibuprofen group (

=-2.971

0.01)

and the efficacy of the ibuprofen group was better than that of the placebo group (

=-2.371

0.05). After treatment

the levels of CD3

CD4

and CD4

/CD8

in TCM group were significantly increased and the levels of CD8

were decreased significantly as compared with those before treatment (

0.01). After treatment

the levels of CD4

and CD4

/CD8

in TCM group were higher (

0.05，

0.01)，while the levels of CD8

were significantly lower than those in ibuprofen group and placebo group (

0.01).

Conclusion

Wenjing Huayu Zhitong therapy can reduce the VAS score and accumulative time of abdominal pain

and improve the dysmenorrhea symptoms in patients with PD. Reversal of the T cell subsets disorder may be one of its mechanisms in treating PD with cold coagulation and blood stasis.

关键词

Keywords

references

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Related Author

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Dong-mei WANG

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SHI Yuanyuan

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Related Institution

Shandong University of TCM

Affiliated Hospital of Shandong University of Traditional Chinese Medicine（TCM）

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