Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide

Yan-wei HAO; Bin LI; Yi ZHANG; Pei-jun XIE; Jun-rong YU; Jin-song XIANG; Dao-yin GONG

doi:10.13422/j.cnki.syfjx.20202002

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Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide

Classic Prescriptions | 更新时间：2020-12-10

- Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 21, Pages: 8-14(2020)
- 作者机构：
  
  成都中医药大学附属医院，成都 610075
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202002
  CLC： R2-0;R22;R285.5;R289
- Received：17 February 2020，
  
  Published Online：21 July 2020，
  
  Published：05 November 2020
- 稿件说明：
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郝彦伟,李斌,张怡等.基于脑肠肽Ghrelin水平探讨远志散对AD大鼠学习记忆能力及进食量的影响[J].中国实验方剂学杂志,2020,26(21):8-14.

HAO Yan-wei,LI Bin,ZHANG Yi,et al.Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(21):8-14.
郝彦伟,李斌,张怡等.基于脑肠肽Ghrelin水平探讨远志散对AD大鼠学习记忆能力及进食量的影响[J].中国实验方剂学杂志,2020,26(21):8-14. DOI： 10.13422/j.cnki.syfjx.20202002.

HAO Yan-wei,LI Bin,ZHANG Yi,et al.Effect of Yuanzhisan on Learning and Memory Abilities and Food Intake of AD Rats Based on Ghrelin Level of Cerebrointestinal Peptide[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(21):8-14. DOI： 10.13422/j.cnki.syfjx.20202002.

摘要

目的

通过制备阿尔茨海默病（AD）大鼠模型，观察远志散对模型大鼠胃促生长素（Ghrelin）表达水平的调控，探讨其防治AD的可能作用机制。

方法

采用随机数字表法将120只SD大鼠分为6组，分别为假手术组，模型组，多奈哌齐组（1.02 mg·kg

-1

），远志散高、中、低剂量组（12，6，3 g·kg

-1

），每组20只，雌雄各半。除假手术组注射同等剂量生理盐水，其余各组大鼠双侧海马立体定向注射

淀粉样蛋白1-40（A

1-40

）诱导AD大鼠模型。连续灌胃治疗10周，观察并记录各组大鼠进食量。灌胃结束后通过Morris水迷宫法检测大鼠学习记忆能力，留取全脑和胃体胃窦，苏木素-伊红（HE）染色观察海马CA1区病理改变，免疫组化法检测Ghrelin表达。

结果

与假手术组比较，模型组大鼠逃避潜伏期显著延长（

0.01），穿越站台次数、有效区域停留时间及运动路程均显著减少（

0.01），海马CA1区细胞排列紊乱、数量减少、胞核固缩，雌雄大鼠进食量明显下降（

0.05，

0.01），海马CA1区、胃黏膜组织中Ghrelin蛋白表达显著降低（

0.01）。与模型组比较，远志散各剂量组大鼠逃避潜伏期均有不同程度的缩短（

0.05，

0.01），穿越站台次数、有效区域停留时间及运动路程均增加（

0.05，

0.01），海马CA1区细胞数目与形态有明显改善，远志散高、中剂量组雌雄大鼠每日进食量显著增加（

0.05，

0.01）；远志散各剂量组大鼠海马CA1区、胃黏膜组织Ghrelin蛋白表达明显增加（

0.05，

0.01）。

结论

远志散能够有效改善AD大鼠学习记忆能力，增加每日进食量，其机制可能与其上调海马CA1区及胃黏膜组织Ghrelin含量有关。

Abstract

Objective

Through the preparation of Alzheimer's disease （AD） rat model， the effect of Yuanzhisan on the expression of Ghrelin was observed， and the possible mechanisms in preventing and treating AD were discussed.

Method

A total of 120 SD rats were randomly divided into sham-operated group， model group， donepezil group（1.02 mg·kg

-1

）， and high， medium and low-dose Yuanzhisan groups （12，6，3 g·kg

-1

）， with 20 rats in each group， including half male and half female. The rats in sham-operated group were injected with normal saline （NS）， and the rats in other groups were injected with

β-

amyloid 1-40 （A

1-40

） in hippocampus to induce the AD rat model. During the 10-week continuous gavage， the food intake of rats in each group was observed and recorded. After the end of gavage， learning and memory abilities of rats were tested by Morris water maze. The whole brain and the gastric body and antrum were collected， the pathologic changes in the CA1 area of hippocampus was assessed by hematoxylin-eosin （HE） staining， and the expression of Ghrelin was detected by immunohistochemistry.

Result

Compared with the sham-operated group， the escape latency time of model group rats increased （

0.01），while times across platform， retention time in effective area and movement distance decreased （

0.01）. The disorder of neurons， the decrease of the neuronal number， and the pyknosis of nucleus were observed in hippocampal CA1 area. The food intake of male and female rats decreased significantly （

0.05，

0.01）. The expression of Ghrelin in hippocampal CA1 area and gastric mucosa decreased significantly （

0.01）. Compared with the model group， the escape latency time of rats in each treatment group was significantly shortened （

0.05，

0.01），whereas times across platform， retention time in effective area and movement distance increased （

0.05，

0.01）. The pathologic change was improved markedly， and the daily food intake of rats in high and medium-dose Yuanzhisan groups increased significantly （

0.05，

0.01）. The protein expression of Ghrelin in hippocampal CA1 area and gastric mucosa increased significantly in each Yuanzhisan group （

0.05，

0.01）.

Conclusion

Yuanzhisan can effectively improve the learning and memory abilities of AD rats and increase the daily intake， which may be related to its up-regulation of Ghrelin content in hippocampal CA1 area and gastric mucosa.

关键词

Keywords

references

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